The Ambiguous Aspects of Oxygen

Round 1

Reviewer 1 Report

 

The manuscript of Napolitano et al., is devoted to interesting aspects of the role of oxygen in the life of the organism. The manuscript contains many facts, but is not well structured. Some chapters overlap. The choice of keywords is not clear. Why is there p450 (only used three times in the text), but no ROS or SOD. In addition, there are many small flaws in the manuscript. Some of them are listed below.

·         Lines 31 – 42. The introduction does not contain any references. Please add.

·         Lines 44 -60. This part of manuscript does not contain any references. Please add.

·         Line 55. ATP is a common abbreviation.

·         Line 130. Please remove the dot before the reference.

·         Line 180. Please remove the dot before the reference.

·         Lines 209 – 228. The part of manuscript does not contain any references. Please add.

·         Line 289. Please use full name for PUFA.

·         412-413. Please use English.

·         Lines 504 – 519. References are missing

·         Line 511. Please use lowercase for O₂

·         Line 521. There are five proteins in the UCP family. Please use “UCPs” if you mean a family of uncoupling proteins.

·         Line 786. Please use full name for GMPc

·         Line 799. I suppose that you mean a nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), not Nκ-B.

·         Lines 613, 825, 829. Please use italics for Latin.

·         Line 850. It may be mentioned that H₂O₂ also oxidizes GSK3beta - another negative regulator of Nrf2.

·         Lines 856-870. This part of manuscript does not contain any references. Please add.

 

·         Lines 871 - 888. It is not clear why this chapter is here, when chapters 5, 6.1, and 6.2 are devoted to the sources of ROS

 

Author Response

The manuscript of Napolitano et al., is devoted to interesting aspects of the role of oxygen in the life of the organism. The manuscript contains many facts, but is not well structured. Some chapters overlap. The choice of keywords is not clear. Why is there p450 (only used three times in the text), but no ROS or SOD. In addition, there are many small flaws in the manuscript. Some of them are listed below.

Thank you so much for the reviewer’s comments. We changed some of the keywords as suggested. And corrected as indicated by the reviewer.

• 1) Lines 31 – 42. The introduction does not contain any references. Please add.

References have been added in the introduction

• 2) Lines 44 -60. This part of manuscript does not contain any references. Please add.

References have been added

• Line 55. ATP is a common abbreviation

We wrote only ATP

• Line 130. Please remove the dot before the reference.

Dot removed

• Line 180. Please remove the dot before the reference

Dot removed

• 3)Lines 209 – 228. The part of manuscript does not contain any references. Please add.

References have been added

• Line 289. Please use full name for PUFA.

The full name for PUFA has been added

• 412-413. Please use English.

Sorry for the mistake. The phrase has been rewritten

• Lines 504 – 519. References are missing

References have been added

• Line 511. Please use lowercase for O₂

corrected

• Line 521. There are five proteins in the UCP family. Please use “UCPs” if you mean a family of uncoupling proteins.

I refer to UCP1. We wrote UCP1 in the revised version

• Line 786. Please use full name for GMPc

Done

• Line 799. I suppose that you mean a nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), not Nκ-B.

Yes, I refer to NF-kB. Thank you for your attention, I corrected

• Lines 613, 825, 829. Please use italics for Latin.

corrected

• Line 850. It may be mentioned that H₂O₂also oxidizes GSK3beta - another negative regulator of Nrf2.

The paragraph has been rewritten and information on GSK3beta has been added

• 4) Lines 856-870. This part of manuscript does not contain any references. Please add.

References have been added

• Lines 871 - 888. It is not clear why this chapter is here, when chapters 5, 6.1, and 6.2 are devoted to the sources of ROS

If the reviewer refers to the paragraph entitled Source of ROS, we wanted to talk about ROS acting as signalling agents. To avoid confusion, in the revised version of the manuscript, we changed the title to “Source of ROS acting as signalling agent” and tried to make it clearer what we were referring to.

 

Reviewer 2 Report

The article “The ambiguous aspects of oxygen” describes various aspects related to the role of oxygen and reactive oxygen species in the functioning of cells. The article is generally well written and contains a lot of interesting information.

However, it requires editing corrections (e.g. Line 412-414: the fragment is not in English; some figures and table 1 are too large and disproportionate).

I also suggest that the Authors could add a chapter on the role of ROS in cancer cells since the greater sensitivity of cancer cells to oxidative stress compared to normal cells is an important feature.

Author Response

Comments and Suggestions for Authors

The article “The ambiguous aspects of oxygen” describes various aspects related to the role of oxygen and reactive oxygen species in the functioning of cells. The article is generally well written and contains a lot of interesting information.

However, it requires editing corrections (e.g. Line 412-414: the fragment is not in English; some figures and table 1 are too large and disproportionate).

Thanks to the reviewer for his comments. We have corrected all the mistakes we found after careful reading.

I also suggest that the Authors could add a chapter on the role of ROS in cancer cells since the greater sensitivity of cancer cells to oxidative stress compared to normal cells is an important feature.

In the conclusion section, we added information about the role of ROS and antioxidants in cancer cells

Round 2

Reviewer 1 Report

The authors have corrected the mistakes. Article can be accepted

Reviewer 2 Report

In my opinion, the manuscript in present form is suitable for publication.