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Biology and Life Sciences Forum
Volume 26
Issue 1
10.3390/Foods2023-15103
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Proceeding Paper

Molecular Identification and Phylogenetic Inference of Lactic Acid Bacteria Isolated from Goat’s Raw Milk Cheese †

by
Laís Carvalho
1,2,
Nathália Fernandes
1,2,
Beatriz Nunes Silva
1,2,
Ursula Gonzales-Barron
1,2 and
Vasco Cadavez
1,2,*
1
Centro de Investigação de Montanha (CIMO), Instituto Politécnico de Bragança, Campus de Santa Apolónia, 5300-253 Bragança, Portugal
2
Laboratório Associado para a Sustentabilidade e Tecnologia em Regiões de Montanha (SusTEC), Instituto Politécnico de Bragança, Campus de Santa Apolónia, 5300-253 Bragança, Portugal
*
Author to whom correspondence should be addressed.
†
Presented at the 4th International Electronic Conference on Foods, 15–30 October 2023; Available online: https://foods2023.sciforum.net/.
Biol. Life Sci. Forum 2023, 26(1), 39; https://doi.org/10.3390/Foods2023-15103
Published: 14 October 2023
(This article belongs to the Proceedings of The 4th International Electronic Conference on Foods)
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Abstract

Lactic acid bacteria (LAB) have a vast genetic potential and are used in food production. The aims of this work were to identify forty-six 16S ribosomal gene sequences of LAB isolated from goat’s raw milk cheeses and to evaluate the phylogenetic relationship between the sequences. Using Sanger sequencing and the BLAST algorithm, the species were identified. Phylogenetic analysis was carried out using the R 4.3.0 software, and the genetic distance was calculated using the MEGA 11 software. The phylogenetic tree revealed variability between sequences of the same species, which may have implications for the technological capabilities of LAB sought for the production of cheeses of standardized quality.
Keywords: lactic acid bacteria; taxonomy; phylogenetic tree; BigDye™ Terminator v3.1 lactic acid bacteria; taxonomy; phylogenetic tree; BigDye™ Terminator v3.1

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MDPI and ACS Style

Carvalho, L.; Fernandes, N.; Silva, B.N.; Gonzales-Barron, U.; Cadavez, V. Molecular Identification and Phylogenetic Inference of Lactic Acid Bacteria Isolated from Goat’s Raw Milk Cheese. Biol. Life Sci. Forum 2023, 26, 39. https://doi.org/10.3390/Foods2023-15103

AMA Style

Carvalho L, Fernandes N, Silva BN, Gonzales-Barron U, Cadavez V. Molecular Identification and Phylogenetic Inference of Lactic Acid Bacteria Isolated from Goat’s Raw Milk Cheese. Biology and Life Sciences Forum. 2023; 26(1):39. https://doi.org/10.3390/Foods2023-15103

Chicago/Turabian Style

Carvalho, Laís, Nathália Fernandes, Beatriz Nunes Silva, Ursula Gonzales-Barron, and Vasco Cadavez. 2023. "Molecular Identification and Phylogenetic Inference of Lactic Acid Bacteria Isolated from Goat’s Raw Milk Cheese" Biology and Life Sciences Forum 26, no. 1: 39. https://doi.org/10.3390/Foods2023-15103

APA Style

Carvalho, L., Fernandes, N., Silva, B. N., Gonzales-Barron, U., & Cadavez, V. (2023). Molecular Identification and Phylogenetic Inference of Lactic Acid Bacteria Isolated from Goat’s Raw Milk Cheese. Biology and Life Sciences Forum, 26(1), 39. https://doi.org/10.3390/Foods2023-15103

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