Molecular Markers in Plant Breeding and Genome Analysis

A special issue of Agriculture (ISSN 2077-0472). This special issue belongs to the section "Crop Genetics, Genomics and Breeding".

Deadline for manuscript submissions: closed (15 February 2023) | Viewed by 2160

Special Issue Editors


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Guest Editor
Genome Research Laboratory, School of Biotechnology, University of Jammu, Jammu 180006, India
Interests: genomics; functional genomics; molecular markers; plant breeding; molecular cytogenetics; genome analysis; transcriptomics; comparative genomics; metabolomics; phylogeny

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Guest Editor
Department of Botany, Central University of Rahya-Suchani (Bagla), Samba 181143, India
Interests: molecular markers; polymorphism; genomic breeding; genomic selection; genomics; genomics-assisted breeding; genotyping platforms; sequence-based trait mapping; sequencing; functional markers; functional sequence characterization; precision plant breeding

Special Issue Information

Dear Colleagues,

Unravelling the structure and level of genetic variation constitutes the first step in the improvement of a plant. In the last four decades or so, greater emphasis has been placed on the application of molecular techniques to explore genetic variations, and as a consequence, specific nucleotide sequences have been used to tag these variations. Such sequences are called molecular markers, which are closely related to the variant sequence/gene of interest. In this way, several markers such as RFLP, AFLP, SNP, SSR, DArT, retrotransposons, CAPS, SCAR, etc. were developed and extensively used not only for diversity assessment but also for linkage mapping, Quantitative Trait Loci (QTL) mapping, chromosome walking, association mapping, and genome analysis, to name a few. With the advent of Next-Generation Sequencing technology, the genome sequences of a large number of plants became available. To further study the genetics of natural variations, Genome-Wide Association Study (GWAS) platforms were developed in several crop plants. In addition, molecular markers have been applied in MAS and genomic selection. In spite of technological advancements, the availability of complete genome sequences of many important crops, and highly advanced molecular tools, in many cases, the desired goals in this field could not be achieved. The inaccurate phenotyping of plants is one of the major causes of this failure. The use of modern tools of Artificial Intelligence paired with high-throughput phenotyping techniques will help in the collection of accurate data, which is a basic requirement is goals regarding breeding are to be achieved successfully. CRISPR technology has revolutionized plant breeding and genetics, and researchers are focusing on editing the genomes of all economically important plants. In the coming years, it is likely that we will see continued innovations in molecular marker technology which will make it more precise, productive, and cost effective, and the underlying biology of various traits of interest will be investigated. Additionally, there is a greater need to integrate molecular-marker-based genotypic data and precise phenotypic data to identify candidate genes and allelic variants controlling various important traits which can increase crop productivity. In addition, the deeper analysis of genomes of economically important plants, especially wild accessions for genetic diversity, the identification of novel genes against different biotic and abiotic stresses, and genome analysis can help in breeding varieties for sustainable agriculture and to ensure the food security of the ever-increasing human population. 

Prof. Dr. Manoj Dhar
Prof. Dr. Brijmohan Singh Bhau
Guest Editors

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Keywords

  • molecular markers
  • gene mapping
  • GWAS
  • MAS
  • QTL
  • genomic selection
  • genotyping
  • genome analysis
  • next-generation sequencing
  • CRISPR

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Published Papers (1 paper)

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Research

21 pages, 756 KiB  
Article
Identification of QTL Associated with Agro-Morphological and Phosphorus Content Traits in Finger Millet under Differential Phosphorus Supply via Linkage Mapping
by Theivanayagam Maharajan, Thumadath Palayullaparambil Ajeesh Krishna, Kasinathan Rakkammal, Muthusamy Ramakrishnan, Stanislaus Antony Ceasar, Manikandan Ramesh and Savarimuthu Ignacimuthu
Agriculture 2023, 13(2), 262; https://doi.org/10.3390/agriculture13020262 - 20 Jan 2023
Cited by 8 | Viewed by 1800
Abstract
Finger millet (Eleusine coracana (L.) Gaertn.) is one of the most important cereal crops for people in Asia and Africa. It supplies energy and nutrients for all people, particularly poor people in these continents. Phosphorus (P) is an essential macronutrient for plant [...] Read more.
Finger millet (Eleusine coracana (L.) Gaertn.) is one of the most important cereal crops for people in Asia and Africa. It supplies energy and nutrients for all people, particularly poor people in these continents. Phosphorus (P) is an essential macronutrient for plant growth and grain quality, so an adequate amount of P is required for healthy plant life. There is considerable evidence that P deficiency affects the growths and yields of all cereals, including finger millet. The present study aimed at identifying the quantitative trait loci (QTL) for various agro-morphological and P-content traits under low P (LP) and high P (HP) conditions through linkage mapping. Two cultivars, IE-2606 (low-P-susceptible) and PR-202 (low-P-tolerant) were used to develop 100 recombinant inbred lines (RILs). In total, 55 QTL, associated with various agro-morphological (primary root length (PRL), shoot fresh weight (SFW), root fresh weight (RFW), shoot dry weight (SDW), root dry weight (RDW), and root hair density (RHD)) and P-content traits (inorganic phosphate content in shoots (PiS), inorganic phosphate content in roots (PiR), and total P content in shoots (TPS)) were detected under LP conditions. Similarly, 37 QTL for various agro-morphological (PRL, RFW, SDW, and root hair length (RHL)) and P-content traits (PiS, PiR, TPS, and total P content in roots (TPR)) were detected under HP conditions. Biotic and abiotic stress-responsive candidate genes linked to a few QTL were also identified. The identified QTL associated with agro-morphological and P-content traits in finger millet under P deficiency could be used in breeding programs to develop finger millet with better P use efficiency (PUE). Furthermore, improving finger millet growth and yield under LP soil will help to reduce Pi application and eutrophication, which may help to improve the lives of poor farmers and maintain sustainable environments in Asia and African countries. Full article
(This article belongs to the Special Issue Molecular Markers in Plant Breeding and Genome Analysis)
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