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Antibiotics
Special Issues
Bacterial Biofilm: Structure, Function, and New Technologies for Biofilm Imaging
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Bacterial Biofilm: Structure, Function, and New Technologies for Biofilm Imaging

A special issue of Antibiotics (ISSN 2079-6382). This special issue belongs to the section "Antibiofilm Strategies".

Deadline for manuscript submissions: closed (31 July 2023) | Viewed by 5554

Special Issue Editor

Dr. Skander Hathroubi

E-Mail Website
Guest Editor
Humboldt-Universität zu Berlin, Cluster of Excellence, Matters of Activity, Institut für Biologie, AG Mikrobiologie Philippstraße 11-13, Haus 22, Berlin, Germany
Interests: biofilm; antibiotic tolerance; OMICS; pathogenesis
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

The term “biofilm” was coined in the 1930s, when Dr. Claude E. Zobell and Dr. Arthur Henrici described how bacteria grow tightly attached to glass slides submerged in pond or sea water. They described a diverse community of bacteria forming a film, firmly attached, covered by a “sheath of gum” and containing some filamentous forms. Later, in the 1970s, Dr. John W. Costerton described these filamentous forms as long fibers extended from the outer membrane of bacteria and tangled and surrounding microbes and mediating their adhesion.

Despite various efforts and years of research, the structure, function, and role of biofilm matrix components that compose the extracellular matrix in conferring protection against many factors, including antibiotics and immune response, remain enigmatic.

Thus, this Special Issue seeks to compile manuscripts that focus on the structure and function of biofilms, characterizing principal matrix components of biofilm and their structural roles and on applying innovative technologies to probe biofilm formation and structural organization.

Dr. Skander Hathroubi
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Antibiotics is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2900 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • biofilm structure
  • biofilm matrix
  • biofilm function
  • antibiotic resistance and tolerance
  • antibiofilm strategies
  • new technology for studying biofilms
  • advancing imaging techniques

Published Papers (2 papers)

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Research

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16 pages, 3639 KiB  
Article
Listeria monocytogenes Biofilms Are Planktonic Cell Factories despite Peracetic Acid Exposure under Continuous Flow Conditions
by Kyle B. Klopper, Elanna Bester and Gideon M. Wolfaardt
Antibiotics 2023, 12(2), 209; https://doi.org/10.3390/antibiotics12020209 - 19 Jan 2023
Cited by 2 | Viewed by 1383
Abstract
Listeria monocytogenes biofilms are ubiquitous in the food-processing environment, where they frequently show resistance against treatment with disinfectants such as peracetic acid (PAA) due to sub-lethal damage resulting in biofilm persistence or the formation of secondary biofilms. L. monocytogenes serovar ½a EGD-e biofilms [...] Read more.
Listeria monocytogenes biofilms are ubiquitous in the food-processing environment, where they frequently show resistance against treatment with disinfectants such as peracetic acid (PAA) due to sub-lethal damage resulting in biofilm persistence or the formation of secondary biofilms. L. monocytogenes serovar ½a EGD-e biofilms were cultivated under continuous flow conditions at 10 °C, 22 °C, and 37 °C and exposed to industrially relevant PAA concentrations. The effect of PAA on biofilm metabolic activity and biomass was monitored in real-time using the CEMS-BioSpec system, in addition to daily measurement of biofilm-derived planktonic cell production. Biofilm-derived planktonic cell yields proved to be consistent with high yields during biofilm establishment (≥106 CFU.mL−1). The exposure of biofilms to the minimum inhibitory PAA concentration (0.16%) resulted in only a brief disruption in whole-biofilm metabolic activity and biofilm biomass accumulation. The recovered biofilm accumulated more biomass and greater activity, but cell yields remained similar. Increasing concentrations of PAA (0.50%, 1.5%, and 4.0%) had a longer-lasting inhibitory effect. Only the maximum dose resulted in a lasting inhibition of biofilm activity and biomass–a factor that needs due consideration in view of dilution in industrial settings. Better disinfection monitoring tools and protocols are required to adequately address the problem of Listeria biofilms in the food-processing environment, and more emphasis should be placed on biofilms serving as a “factory” for cell proliferation rather than only a survival mechanism. Full article
(This article belongs to the Special Issue Bacterial Biofilm: Structure, Function, and New Technologies for Biofilm Imaging)
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Review

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19 pages, 975 KiB  
Review
Biofilm of Helicobacter pylori: Life Cycle, Features, and Treatment Options
by Yasmine Elshenawi, Shuai Hu and Skander Hathroubi
Antibiotics 2023, 12(8), 1260; https://doi.org/10.3390/antibiotics12081260 - 31 Jul 2023
Cited by 6 | Viewed by 3559
Abstract
Helicobacter pylori is a gastric pathogen that infects nearly half of the global population and is recognized as a group 1 carcinogen by the Word Health Organization. The global rise in antibiotic resistance has increased clinical challenges in treating H. pylori infections. Biofilm [...] Read more.
Helicobacter pylori is a gastric pathogen that infects nearly half of the global population and is recognized as a group 1 carcinogen by the Word Health Organization. The global rise in antibiotic resistance has increased clinical challenges in treating H. pylori infections. Biofilm growth has been proposed to contribute to H. pylori’s chronic colonization of the host stomach, treatment failures, and the eventual development of gastric diseases. Several components of H. pylori have been identified to promote biofilm growth, and several of these may also facilitate antibiotic tolerance, including the extracellular matrix, outer membrane proteins, shifted morphology, modulated metabolism, efflux pumps, and virulence factors. Recent developments in therapeutic approaches targeting H. pylori biofilm have shown that synthetic compounds, such as small molecule drugs and plant-derived compounds, are effective at eradicating H. pylori biofilms. These combined topics highlight the necessity for biofilm-based research in H. pylori, to improve current H. pylori-targeted therapeutic approaches and alleviate relative public health burden. In this review we discuss recent discoveries that have decoded the life cycle of H. pylori biofilms and current biofilm-targeted treatment strategies. Full article
(This article belongs to the Special Issue Bacterial Biofilm: Structure, Function, and New Technologies for Biofilm Imaging)
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