Antioxidants

51 pages, 2228 KiB

Open AccessSystematic Review

Antioxidant Therapy in Inflammatory Bowel Diseases: How Far Have We Come and How Close Are We?

by Lylian Ellen Militão dos Santos Xavier, Thays Cristhyna Guimaraes Reis, Amylly Sanuelly da Paz Martins, Juliana Célia de Farias Santos, Nassib Bezerra Bueno, Marília Oliveira Fonseca Goulart and Fabiana Andréa Moura

Antioxidants 2024, 13(11), 1369; https://doi.org/10.3390/antiox13111369 (registering DOI) - 8 Nov 2024

Abstract

Inflammatory bowel diseases (IBD) pose a growing public health challenge with unclear etiology and limited efficacy of traditional pharmacological treatments. Alternative therapies, particularly antioxidants, have gained scientific interest. This systematic review analyzed studies from MEDLINE, Cochrane, Web of Science, EMBASE, and Scopus using [...] Read more.

Inflammatory bowel diseases (IBD) pose a growing public health challenge with unclear etiology and limited efficacy of traditional pharmacological treatments. Alternative therapies, particularly antioxidants, have gained scientific interest. This systematic review analyzed studies from MEDLINE, Cochrane, Web of Science, EMBASE, and Scopus using keywords like “Inflammatory Bowel Diseases” and “Antioxidants.” Initially, 925 publications were identified, and after applying inclusion/exclusion criteria—covering studies from July 2015 to June 2024 using murine models or clinical trials in humans and evaluating natural or synthetic substances affecting oxidative stress markers—368 articles were included. This comprised 344 animal studies and 24 human studies. The most investigated antioxidants were polyphenols and active compounds from medicinal plants (n = 242; 70.3%). The review found a strong link between oxidative stress and inflammation in IBD, especially in studies on nuclear factor kappa B and nuclear factor erythroid 2-related factor 2 pathways. However, it remains unclear whether inflammation or oxidative stress occurs first in IBD. Lipid peroxidation was the most studied oxidative damage, followed by DNA damage. Protein damage was rarely investigated. The relationship between antioxidants and the gut microbiota was examined in 103 animal studies. Human studies evaluating oxidative stress markers were scarce, reflecting a major research gap in IBD treatment. PROSPERO registration: CDR42022335357 and CRD42022304540. Full article

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17 pages, 1592 KiB

Open AccessArticle

Modelling the Repair of Carbon-Centered Protein Radicals by Phenolic Antioxidants

by Max Walton-Raaby, Tyler Floen and Nelaine Mora-Diez

Antioxidants 2024, 13(11), 1368; https://doi.org/10.3390/antiox13111368 (registering DOI) - 8 Nov 2024

Abstract

Oxidative stress is a biological process that has been linked to many diseases, hence understanding how to prevent and repair it is essential to medicine. The thermodynamics and kinetics of the repair reactions of radically damaged leucine (a lateral chain in a simplified [...] Read more.

Oxidative stress is a biological process that has been linked to many diseases, hence understanding how to prevent and repair it is essential to medicine. The thermodynamics and kinetics of the repair reactions of radically damaged leucine (a lateral chain in a simplified protein environment) by twenty phenolic antioxidants are studied at the M06-2X(SMD)/6-31++G(d,p) level of theory in water and pentyl ethanoate. The two repair mechanisms modelled are formal-hydrogen atom transfer (f-HAT) and single electron transfer (SET). Although all f-HAT reactions are thermodynamically favourable, only one of the phenols produced rate constants in the diffusion limit, exhibiting biological relevance. SET is not suspected to be an important repair pathway for the phenols studied. We show that the Bell–Evans–Polanyi principle, which relates thermodynamics and kinetics properties for a reaction, breaks down when comparing between the solvents, protein repair sites, and the phenolic antioxidants. While thermodynamic data can be used as valuable screening tools, the kinetic calculation of rate constants in solution is crucial for enhancing the biological relevance of theoretical studies. Full article

(This article belongs to the Section Natural and Synthetic Antioxidants)

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12 pages, 1350 KiB

Open AccessArticle

Moringa oleifera Lam. Leaf Peptides: Antioxidant and Antiproliferative Activity in Human Colon Cancer Caco-2 Cell Line

by Sara Avilés-Gaxiola, Laura Aracely Contreras-Angulo, Israel García-Aguiar and J. Basilio Heredia

Antioxidants 2024, 13(11), 1367; https://doi.org/10.3390/antiox13111367 - 8 Nov 2024

Abstract

Reactive oxygen species are produced as part of the cellular metabolism. However, lifestyle can promote an excess in their concentration. Free radicals react with DNA, promoting the appearance of cancer cells. Therefore, natural antioxidants have been suggested as an alternative to prevent this [...] Read more.

Reactive oxygen species are produced as part of the cellular metabolism. However, lifestyle can promote an excess in their concentration. Free radicals react with DNA, promoting the appearance of cancer cells. Therefore, natural antioxidants have been suggested as an alternative to prevent this disorder. Peptides are protein fragments that have been produced from various plants. In previous work, Moringa oleifera leaf peptides (MOPHs) with antioxidant potential were generated and identified. However, the spectrophotometric methods used to evaluate their antioxidant activity do not fully reflect its potential. In this work, the antioxidant activity of MOPHs was assessed by the ferric reducing antioxidant power assay (FRAP) and cellular antioxidant activity method on the human colon cancer cell line Caco-2. Also, their antiproliferative activity was evaluated. The MOPHs exhibited a FRAP activity of 1435 µmol TE/g, and at 500 µg/mL; the peptides did not show a cytotoxic effect on healthy colon CCD-18Co cells. Moreover, the MOPHs increased Caco-2 antioxidative activity to a greater extent by 73.45% and 83.62% at 250 and 500 µg/mL, respectively. Regarding cellular proliferation, the MOPHs inhibited it by 78.19% and 90.20% at 200 and 500 µg/mL, respectively. These findings highlight the potential of Moringa oleifera leaf peptides as functional ingredients with significant health benefits, demonstrating antioxidant and antiproliferative properties. Full article

(This article belongs to the Special Issue Natural Products: Biological, Antioxidant Properties and Health Effects—3rd Edition)

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Graphical abstract

17 pages, 1822 KiB

Open AccessReview

Domoic Acid: A Review of Its Cytogenotoxicity Within the One Health Approach

by Goran Gajski, Marko Gerić, Ana Baričević and Mirta Smodlaka Tanković

Antioxidants 2024, 13(11), 1366; https://doi.org/10.3390/antiox13111366 - 8 Nov 2024

Abstract

In this review, we toxicologically assessed the naturally occurring toxin domoic acid. We used the One Health approach because the impact of domoic acid is potentiated by climate change and water pollution on one side, and reflected in animal health, food security, human [...] Read more.

In this review, we toxicologically assessed the naturally occurring toxin domoic acid. We used the One Health approach because the impact of domoic acid is potentiated by climate change and water pollution on one side, and reflected in animal health, food security, human diet, and human health on the other. In a changing environment, algal blooms are more frequent. For domoic acid production, the growth of Pseudo-nitzschia diatoms is of particular interest. They produce this toxin, whose capability of accumulation and biomagnification through the food web impacts other organisms in the ecosystem. Domoic acid targets nervous system receptors inducing amnestic shellfish poisoning, among other less severe health-related problems. However, the impact of domoic acid on non-target cells is rather unknown, so we reviewed the currently available literature on cytogenetic effects on human and animal cells. The results of different studies indicate that domoic acid has the potential to induce early molecular events, such as oxidative imbalance and DNA damage, thus posing an additional threat which needs to be thoroughly addressed and monitored in the future. Full article

(This article belongs to the Special Issue Oxidative Stress Responses to Freshwater and Marine Toxins: From Molecular Mechanisms to Ecological Impacts)

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3 pages, 180 KiB

Open AccessEditorial

Leveraging the ROS–TME Axis for Cancer Treatment

by Kostas A. Papavassiliou, Amalia A. Sofianidi, Vassiliki A. Gogou and Athanasios G. Papavassiliou

Antioxidants 2024, 13(11), 1365; https://doi.org/10.3390/antiox13111365 - 7 Nov 2024

Abstract

The discovery of reactive oxygen species (ROS) dates back to the early 20th century [...] Full article

(This article belongs to the Section ROS, RNS and RSS)

37 pages, 7050 KiB

Open AccessReview

Role of Plant Phytochemicals: Resveratrol, Curcumin, Luteolin and Quercetin in Demyelination, Neurodegeneration, and Epilepsy

by Mikołaj Grabarczyk, Weronika Justyńska, Joanna Czpakowska, Ewa Smolińska, Aleksandra Bielenin, Andrzej Glabinski and Piotr Szpakowski

Antioxidants 2024, 13(11), 1364; https://doi.org/10.3390/antiox13111364 - 7 Nov 2024

Abstract

Polyphenols are an important group of biologically active compounds present in almost all food sources of plant origin and are primarily known for their anti-inflammatory and antioxidative capabilities. Numerous studies have indicated their broad spectrum of pharmacological properties and correlations between their increased [...] Read more.

Polyphenols are an important group of biologically active compounds present in almost all food sources of plant origin and are primarily known for their anti-inflammatory and antioxidative capabilities. Numerous studies have indicated their broad spectrum of pharmacological properties and correlations between their increased supply in the human diet and lower prevalence of various disorders. The positive effects of polyphenols application are mostly discussed in terms of cardiovascular system well-being. However, in recent years, they have also increasingly mentioned as prophylactic and therapeutic factors in the context of neurological diseases, being able to suppress the progression of such disorders and soothe accompanying symptoms. Among over 8000 various compounds, that have been identified, the most widely examined comprise resveratrol, curcumin, luteolin and quercetin. This review focuses on in vitro assessments, animal models and clinical trials, reflecting the most actual state of knowledge, of mentioned polyphenols’ medicinal capabilities in epilepsy, demyelinating and neurodegenerative diseases of the central nervous system. Full article

(This article belongs to the Special Issue Role of Natural Antioxidants on Neuroprotection)

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13 pages, 1051 KiB

Open AccessReview

Myokines and Their Potential Protective Role Against Oxidative Stress in Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD)

by José Luis Bucarey, Isis Trujillo-González, Evan M. Paules and Alejandra Espinosa

Antioxidants 2024, 13(11), 1363; https://doi.org/10.3390/antiox13111363 - 7 Nov 2024

Abstract

Myokines, bioactive peptides released by skeletal muscle, have emerged as crucial regulators of metabolic and protective pathways in peripheral tissues, particularly in combating oxidative stress and inflammation. Their plasma concentration significantly increases following exercise, offering valuable insights into the role of physical activity [...] Read more.

Myokines, bioactive peptides released by skeletal muscle, have emerged as crucial regulators of metabolic and protective pathways in peripheral tissues, particularly in combating oxidative stress and inflammation. Their plasma concentration significantly increases following exercise, offering valuable insights into the role of physical activity in preventing sarcopenia and mitigating metabolic diseases, including obesity, diabetes, and metabolic dysfunction-associated steatotic liver disease (MASLD). This review focuses on discussing the roles of specific myokines in activating intracellular signaling pathways within the liver, which confer protection against steatosis and lipid peroxidation. We detail the mechanism underlying lipid peroxidation and highlight the liver’s antioxidant defenses, such as glutathione (GSH) and glutathione peroxidase 4 (GPX4), which are pivotal in reducing ferroptosis. Furthermore, we provide an in-depth analysis of key myokines, including myostatin, brain-derived neurotrophic factor (BDNF), and irisin, among others, and their potential impact on liver function. Finally, we discuss the molecular mechanisms through which these myokines influence oxidate stress and lipid metabolism, emphasizing their capacity to modulate antioxidant responses in the liver. Finally, we underscore the therapeutic potential of exercise as a non-pharmacological intervention to enhance myokine release, thereby preventing the progression of MASD through improved hepatic antioxidant defenses. This review represents a comprehensive perspective on the intersection of exercise, myokine biology, and liver health. Full article

(This article belongs to the Special Issue Oxidative Stress and Liver Disease)

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23 pages, 2402 KiB

Open AccessArticle

Antioxidative Status and Meat Quality Traits as Affected by Dietary Supplementation of Finishing Pigs with Natural Phenolics

by Małgorzata Muzolf-Panek, Anita Zaworska-Zakrzewska, Anna Czech, Dariusz Lisiak and Małgorzata Kasprowicz-Potocka

Antioxidants 2024, 13(11), 1362; https://doi.org/10.3390/antiox13111362 - 7 Nov 2024

Abstract

This work investigated the effect of a plant-based phenolic supplement on the color, myoglobin forms, lipid oxidation, and antioxidative status of fresh and stored (10 days at 4 °C) meat (Longissimus thoracis et lumborum), as well as the antioxidative status of [...] Read more.

This work investigated the effect of a plant-based phenolic supplement on the color, myoglobin forms, lipid oxidation, and antioxidative status of fresh and stored (10 days at 4 °C) meat (Longissimus thoracis et lumborum), as well as the antioxidative status of the blood and liver. The sensory quality of the meat was also evaluated for color, aroma, texture, juiciness, and palatability. Twenty-four finishing pigs, divided into two groups, were fed a basal diet and a diet with a phenolic supplement (0.1%). The supplementation increased the redness of the meat (+36% for a* and +28% for redness index), the myoglobin (Mb) content (+7%), the antioxidant activity, and the juiciness. The treatment increased the antioxidant status of meat, reflected by superoxide dismutase (SOD) activity and total glutathione (GSH + GSSG). The catalase and SOD activities and GSH + GSSG of the blood and liver were also elevated in the supplemented samples when compared to the control group. A significant effect of time was observed for all tested parameters (pH, color attributes, Mb forms, the antioxidant activity, lipid oxidation) except for the Mb content. For the stored samples, only TBARSs (thiobarbituric acid reagent substances) were affected by the diet. The slope of the plot for TBARS changes with time was significantly different between the control and treated groups (p = 0.017), which indicated a significant effect of dietary supplementation. A higher rate of lipid oxidation was observed in the control samples. Full article

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20 pages, 4428 KiB

Open AccessArticle

SOD1 Is an Integral Yet Insufficient Oxidizer of Hydrogen Sulfide in Trisomy 21 B Lymphocytes and Can Be Augmented by a Pleiotropic Carbon Nanozyme

by Karthik Mouli, Anton V. Liopo, Larry J. Suva, Kenneth R. Olson, Emily A. McHugh, James M. Tour, Paul J. Derry and Thomas A. Kent

Antioxidants 2024, 13(11), 1361; https://doi.org/10.3390/antiox13111361 - 7 Nov 2024

Abstract

Down syndrome (DS) is a multisystemic disorder that includes accelerated aging caused by trisomy 21. In particular, overexpression of cystathionine-β-synthase (CBS) is linked to excess intracellular hydrogen sulfide (H₂S), a mitochondrial toxin at higher concentrations, which impairs cellular viability. Concurrent overexpression [...] Read more.

Down syndrome (DS) is a multisystemic disorder that includes accelerated aging caused by trisomy 21. In particular, overexpression of cystathionine-β-synthase (CBS) is linked to excess intracellular hydrogen sulfide (H₂S), a mitochondrial toxin at higher concentrations, which impairs cellular viability. Concurrent overexpression of superoxide dismutase 1 (SOD1) may increase oxidative stress by generating excess hydrogen peroxide (H₂O₂) while also mitigating the toxic H₂S burden via a non-canonical sulfide-oxidizing mechanism. We investigated the phenotypic variability in basal H₂S levels in relation to DS B lymphocyte cell health and SOD1 in H₂S detoxification. The H₂S levels were negatively correlated with the DS B lymphocyte growth rates but not with CBS protein. Pharmacological inhibition of SOD1 using LCS-1 significantly increased the H₂S levels to a greater extent in DS cells while also decreasing the polysulfide products of H₂S oxidation. However, DS cells exhibited elevated H₂O₂ and lipid peroxidation, representing potential toxic consequences of SOD1 overexpression. Treatment of DS cells with a pleiotropic carbon nanozyme (pleozymes) decreased the total oxidative stress and reduced the levels of the H₂S-generating enzymes CBS and 3-mercaptopyruvate sulfurtransferase (MPST). Our results indicate that pleozymes may bridge the protective and deleterious effects of DS SOD1 overexpression on H₂S metabolism and oxidative stress, respectively, with cytoprotective benefits. Full article

(This article belongs to the Special Issue Role of Super Oxide Dismutase (SOD)1 Isoenzyme and Reactive Oxygen Species (ROS) Generation in Cellular Pathophysiology)

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14 pages, 3169 KiB

Open AccessArticle

Enhancing of Rabbit Sperm Cryopreservation with Antioxidants Mito-Tempo and Berberine

by Lenka Kuželová, Andrea Svoradová, Andrej Baláži, Jaromír Vašíček, Vladimír Langraf, Adriana Kolesárová, Petr Sláma and Peter Chrenek

Antioxidants 2024, 13(11), 1360; https://doi.org/10.3390/antiox13111360 - 6 Nov 2024

Abstract

Cryopreservation plays a critical role in animal breeding and the conservation of endangered species, but it often compromises sperm characteristics such as morphology, motility, and viability due to oxidative stress. This study explores the antioxidative effect of Mito-Tempo (MT) and Berberine (BER) to [...] Read more.

Cryopreservation plays a critical role in animal breeding and the conservation of endangered species, but it often compromises sperm characteristics such as morphology, motility, and viability due to oxidative stress. This study explores the antioxidative effect of Mito-Tempo (MT) and Berberine (BER) to enhance post-thaw sperm quality in rabbits. Pooled rabbit sperm samples were supplemented with different concentrations (0.0, 0.5, 5, 10, 50 µmol/L) of MT and BER. Sperm motility was evaluated using computer-assisted semen analysis, while viability, apoptosis, reactive oxygen species (ROS) levels, acrosome integrity, and mitochondrial function were assessed through flow cytometry. The results revealed that MT at 5 and 10 µmol/L and BER at 10 µmol/L significantly improved total and progressive motility, mitochondrial activity, and sperm viability compared to the control group. Furthermore, 10 µmol/L BER enhanced acrosome integrity, while both 5 µmol/L MT and 10 µmol/L BER effectively reduced ROS levels and apoptosis. This study is the first to demonstrate the protective effects of MT and BER on rabbit sperm during cryopreservation. By mitigating oxidative stress and reducing apoptosis, these antioxidants markedly improved post-thaw sperm quality, positioning MT and BER as promising agents for improving sperm cryosurvival. Full article

(This article belongs to the Section Health Outcomes of Antioxidants and Oxidative Stress)

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26 pages, 25856 KiB

Open AccessArticle

TORC1 Regulates Thermotolerance via Modulating Metabolic Rate and Antioxidant Capacity in Scallop Argopecten irradians irradians

by Longfei Chu, Ancheng Liu, Jiaxi Chang, Junhao Zhang, Xiujiang Hou, Xinghai Zhu, Qiang Xing and Zhenmin Bao

Antioxidants 2024, 13(11), 1359; https://doi.org/10.3390/antiox13111359 - 6 Nov 2024

Abstract

Target of rapamycin complex 1 (TORC1) is a key regulator of metabolism in eukaryotes across multiple pathways. Although TORC1 has been extensively studied in vertebrates and some invertebrates, research on this complex in scallops is limited. In this study, we identified the genes [...] Read more.

Target of rapamycin complex 1 (TORC1) is a key regulator of metabolism in eukaryotes across multiple pathways. Although TORC1 has been extensively studied in vertebrates and some invertebrates, research on this complex in scallops is limited. In this study, we identified the genes encoding TORC1 complex subunits in the scallop Argopecten irradians irradians through genome-wide in silico scanning. Five genes, including TOR, RAPTOR, LST8, DEPTOR, and PRAS40, that encode the subunits of TORC1 complex were identified in the bay scallop. We then conducted structural characterization and phylogenetic analysis of the A. i. irradians TORC1 (AiTORC1) subunits to determine their structural features and evolutionary relationships. Next, we analyzed the spatiotemporal expressions of AiTORC1-coding genes during various embryo/larvae developmental stages and across different tissues in healthy adult scallops. The results revealed stage- and tissue-specific expression patterns, suggesting diverse roles in development and growth. Furthermore, the regulation of AiTORC1-coding genes was examined in temperature-sensitive tissues (the mantle, gill, hemocyte, and heart) of bay scallops exposed to high-temperature (32 °C) stress over different durations (0 h, 6 h, 12 h, 24 h, 3 d, 6 d, and 10 d). The expression of AiTORC1-coding genes was predominantly suppressed in the hemocyte but was generally activated in the mantle, gill, and heart, indicating a tissue-specific response to heat stress. Finally, functional validation was performed using the TOR inhibitor rapamycin to suppress AiTORC1, leading to an enhanced catabolism, a decreased antioxidant capacity, and a significant reduction in thermotolerance in bay scallops. Collectively, this study elucidates the presence, structural features, evolutional relationships, expression profiles, and roles in antioxidant capacity and metabolism regulation of AiTORC1 in the bay scallop, providing a preliminary understanding of its versatile functions in response to high-temperature challenges in marine mollusks. Full article

(This article belongs to the Special Issue The Role of Oxidative Stress in Aquaculture)

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21 pages, 1799 KiB

Open AccessArticle

Mediterranean Diet and Olive Oil Redox Interactions on Lactate Dehydrogenase Mediated by Gut Oscillibacter in Patients with Long-COVID-19 Syndrome

by Amanda Cuevas-Sierra, Victor de la O, Andrea Higuera-Gómez, Lourdes Chero-Sandoval, Begoña de Cuevillas, María Martínez-Urbistondo, Victor Moreno-Torres, Ilduara Pintos-Pascual, Raquel Castejón and J. Alfredo Martínez

Antioxidants 2024, 13(11), 1358; https://doi.org/10.3390/antiox13111358 - 6 Nov 2024

Abstract

Chronic viral inflammation is associated with oxidative stress and changes in gut microbiota. The Mediterranean diet (MD), with recognized anti-inflammatory and antioxidant properties, modulates gut microorganisms, specifically on the interaction between extra virgin olive oil, a key component of the MD with well-documented [...] Read more.

Chronic viral inflammation is associated with oxidative stress and changes in gut microbiota. The Mediterranean diet (MD), with recognized anti-inflammatory and antioxidant properties, modulates gut microorganisms, specifically on the interaction between extra virgin olive oil, a key component of the MD with well-documented antioxidant effects. This study investigated the influence of adherence to MD and antioxidant-rich foods (extra virgin olive oil) on biochemical, inflammatory, and microbiota profiles in patients with chronic inflammation defined as a prolonged inflammatory response due to immune dysregulation following the acute phase of the viral infection. Participants were classified into low (n = 54) and high (n = 134) MD adherence groups (cut-off of 7 points based on previous studies utilizing the same threshold in the assessment of MD adherence). Gut microbiota was sequenced using the 16S technique, and the adherence to MD was assessed using a validated questionnaire for a Spanish population. High adherence to the MD was linked to significant improvements in inflammatory and oxidative stress markers, including reductions in LDL-cholesterol, glucose, and lactate dehydrogenase (LDH) levels, an indicative of redox balance, as well as a significant higher consumption of antioxidant foods. Moreover, gut microbiota analysis revealed distinct compositional shifts and a lower abundance of the Oscillibacter genus in the high adherence group. Notably, a significant interaction was observed between MD adherence and extra virgin olive oil consumption, with Oscillibacter abundance influencing LDH levels, suggesting that the MD antioxidant properties may modulate inflammation through gut microbiota-mediated mechanisms. These findings provide new evidence that adherence to the Mediterranean diet can reduce inflammatory markers in patients with long-COVID-19, a population that has not been extensively studied, while also highlighting the potential role of the bacterial genus Oscillibacter in modulating this effect. Full article

(This article belongs to the Special Issue Antioxidant Compounds and Health Benefits of Mediterranean Functional Food)

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15 pages, 1489 KiB

Open AccessArticle

The Impact of Acute Ammonia Nitrogen Stress on the Gill Tissue Structure and Antioxidant Ability of Gills and Red and White Muscle in Juvenile Yellowfin Tuna (Thunnus albacares)

by Yongyue Sun, Zhengyi Fu, Xuancheng Liu and Zhenhua Ma

Antioxidants 2024, 13(11), 1357; https://doi.org/10.3390/antiox13111357 - 6 Nov 2024

Abstract

To explore the impacts of acute ammonia nitrogen (NH₃-N) stress on gill structure and the antioxidant ability of red and white muscles in juvenile yellowfin tuna (Thunnus albacares), this study used natural seawater as a control, establishing two experimental [...] Read more.

To explore the impacts of acute ammonia nitrogen (NH₃-N) stress on gill structure and the antioxidant ability of red and white muscles in juvenile yellowfin tuna (Thunnus albacares), this study used natural seawater as a control, establishing two experimental NH₃-N groups at 5 and 10 mg/L. Gills and red and white muscle were taken at 6, 24, and 36 h for the determination of malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GHS-PX) levels, and to observe gill structure. The results indicated that, with increasing time, the MDA concentration and CAT activity in the gills of the 5 mg/L group showed a trend of first increasing and then decreasing, while SOD activity exhibited a downward trend. In the 10 mg/L group, MDA concentration showed an increasing trend, while SOD, CAT, and GSH-PX activities demonstrated a trend of first increasing and then decreasing. In the 5 mg/L group, the MDA concentration and GSH-PX activity in the red muscle showed an increasing trend. In the 10 mg/L group, MDA concentration and SOD and CAT activities exhibited a downward trend. In the 5 mg/L group, the MDA concentration and SOD activity in the white muscle showed a downward trend, while CAT activity exhibited an increasing trend. In the 10 mg/L group, MDA concentration and CAT activity demonstrated a trend of first increasing and then decreasing, while SOD activity showed a downward trend. Ammonia nitrogen can lead to necrosis and shedding of gill epithelial cells, cell vacuolation, edema, as well as proliferation, hypertrophy, and fusion of secondary lamellae. This study demonstrates that NH₃-N can alter gill structure and reduce the antioxidant ability of gills and red–white muscle. The findings provide scientific data that can support the aquaculture and recirculating aquaculture systems of juvenile tuna. Full article

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19 pages, 3982 KiB

Open AccessArticle

Effects of Oxidized Soybean Meal and Oxidized Soybean Oil on the Muscle Oxidative Stability, Flesh Quality, Amino Acid Profile, and Fatty Acid Profile of Megalobrama amblycephala

by Yangyang Huang, Xiufei Cao, Wenbin Liu, Guangzhen Jiang and Aimin Wang

Antioxidants 2024, 13(11), 1356; https://doi.org/10.3390/antiox13111356 - 6 Nov 2024

Abstract

This study aimed to investigate the effects of oxidized soybean meal and oxidized soybean oil on the muscle oxidative stability, flesh quality, amino acid profile, and fatty acid profile of blunt snout bream Megalobrama amblycephala. Oxidized soybean meal and oxidized soybean oil [...] Read more.

This study aimed to investigate the effects of oxidized soybean meal and oxidized soybean oil on the muscle oxidative stability, flesh quality, amino acid profile, and fatty acid profile of blunt snout bream Megalobrama amblycephala. Oxidized soybean meal and oxidized soybean oil were obtained from fresh soybean meal (FSM) and fresh soybean oil (FSO) by heating. In the experimental diet, the proportions of oxidized soybean meal (OSM) and oxidized soybean oil (OSO) were 30% and 4.19%, respectively. The feeding trial was conducted for 8 weeks. The findings revealed that both OSM and OSO reduced glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), catalase (CAT), hardness, chewiness, and oxymyoglobin (OxyMb) and elevated the content of malondialdehyde (MDA), protein carbonyl (PC), and metmyoglobin (MetMb) in the muscle. OSM notably decreased the content of muscle essential amino acids (EAAs), nonessential amino acids (NEAAs), delicious amino acids (DAAs), and total amino acids (TAAs) compared with CON and OSO. Compared with CON and OSM, OSO significantly reduced the content of elaidic acid (C18:1n9t), linoelaidic acid (C18:2n6c), polyunsaturated fatty acids (PUFAs), ω-6 PUFAs, and the ratio of ω-6/ω-3, while stearic acid (C18:0), γ-linolenic acid (C18:3n6) and saturated fatty acids (SFAs) were significantly elevated. In conclusion, this study demonstrated that both OSM and OSO negatively impacted muscle antioxidant capacity and flesh quality. Moreover, OSM adversely affected the amino acid profile of the muscle, while OSO impaired the fatty acid profile. Full article

(This article belongs to the Special Issue Oxidative Stress and Nutrition in Aquatic Animals)

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25 pages, 5589 KiB

Open AccessArticle

Investigation of the Fermentation Process of Moringa oleifera Leaves and Its Effects on the Growth Performance, Antioxidant Capacity, and Intestinal Microbiome of Procambarus clarkii

by Zhengzhong Li, Weizhu Luo, Qunlan Zhou, Cunxin Sun, Xiaochuan Zheng, Bo Liu, Kaunda Mpange, Aimin Zhu and Aimin Wang

Antioxidants 2024, 13(11), 1355; https://doi.org/10.3390/antiox13111355 - 5 Nov 2024

Abstract

Moringa oleifera is renowned for its high antioxidant activity. However, few studies have been conducted on its effects on aquatic animals. The aim of this experiment was to investigate the optimal fermentation process of M. oleifera leaves and to evaluate the effects of [...] Read more.

Moringa oleifera is renowned for its high antioxidant activity. However, few studies have been conducted on its effects on aquatic animals. The aim of this experiment was to investigate the optimal fermentation process of M. oleifera leaves and to evaluate the effects of fermented M. oleifera leaves on crayfish (9.11 ± 0.3 g) in terms of growth performance, antioxidant capacity, and gut microbiological parameters. By optimizing the fermenting material/water ratio, fermentation time, temperature, and strain, the optimal fermentation conditions of a 10% water ratio + 48 h + 30 °C + inoculation with 2% B. amyloliquefaciens (10⁷ CFU mL⁻¹) were obtained. These conditions resulted in notable increases in the contents of the total protein, total phenols, flavonoids, and amino acids (p < 0.05) while also leading to a notable decrease in the content of tannins in contrast to those of unfermented M. oleifera leaves (p < 0.05). The fermented M. oleifera (FMO) leaves were incorporated at five concentrations, including 0% (control (CT)), 0.25% (0.25FMO), 0.5% (0.5FMO), 1% (1FMO), and 2% (2FMO). The results showed that the 1FMO group performed better in terms of the final body weight (FBW), weight gain rate (WGR), and specific weight gain rate (SGR) compared with the CT group (p < 0.05). In addition, amylase and lipase activities were significantly higher in the 1FMO and 2FMO groups compared with the other groups (p < 0.05). The fermented M. oleifera leaves significantly increased the catalase (CAT) activity in the crayfish (p < 0.05). The superoxide dismutase (SOD) activity was significantly increased in the 0.25FMO, 1FMO, and 2FMO groups, and the malondialdehyde (MDA) content was significantly decreased while the glutathione peroxidase (GSH-PX) content was significantly increased in the 0.5FMO, 1FMO, and 2FMO groups (p < 0.05). Furthermore, the 1FMO group was observed to significantly increase the abundance of Firmicutes while simultaneously reducing the abundance of Aeromonas (p < 0.05) and adjusting the structure of the intestinal microbiome. In conclusion, this study established the optimal fermentation conditions for M. oleifera and obtained a product with high nutrient and low tannin contents. Furthermore, the incorporation of 1% FMO was demonstrated to facilitate growth, enhance the antioxidant capacity, and optimize the gut microbiology in crayfish. Full article

(This article belongs to the Special Issue Oxidative Stress and Nutrition in Aquatic Animals)

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22 pages, 3183 KiB

Open AccessReview

The Link Between Paraquat and Demyelination: A Review of Current Evidence

by Renata Silva, Ana Filipa Sobral, Ricardo Jorge Dinis-Oliveira and Daniel José Barbosa

Antioxidants 2024, 13(11), 1354; https://doi.org/10.3390/antiox13111354 - 5 Nov 2024

Abstract

Paraquat (1,1′-dimethyl-4,4′-bipyridilium dichloride), a widely used bipyridinium herbicide, is known for inducing oxidative stress, leading to extensive cellular toxicity, particularly in the lungs, liver, kidneys, and central nervous system (CNS), and is implicated in fatal poisonings. Due to its biochemical similarities with the [...] Read more.

Paraquat (1,1′-dimethyl-4,4′-bipyridilium dichloride), a widely used bipyridinium herbicide, is known for inducing oxidative stress, leading to extensive cellular toxicity, particularly in the lungs, liver, kidneys, and central nervous system (CNS), and is implicated in fatal poisonings. Due to its biochemical similarities with the neurotoxin 1-methyl-4-phenylpyridinium (MPP+), paraquat has been used as a Parkinson’s disease model, although its broader neurotoxic effects suggest the participation of multiple mechanisms. Demyelinating diseases are conditions characterized by damage to the myelin sheath of neurons. They affect the CNS and peripheral nervous system (PNS), resulting in diverse clinical manifestations. In recent years, growing concerns have emerged about the impact of chronic, low-level exposure to herbicides on human health, particularly due to agricultural runoff contaminating drinking water sources and their presence in food. Studies indicate that paraquat may significantly impact myelinating cells, myelin-related gene expression, myelin structure, and cause neuroinflammation, potentially contributing to demyelination. Therefore, demyelination may represent another mechanism of neurotoxicity associated with paraquat, which requires further investigation. This manuscript reviews the potential association between paraquat and demyelination. Understanding this link is crucial for enhancing strategies to minimize exposure and preserve public health. Full article

(This article belongs to the Special Issue Oxidative Stress and Antioxidants in Neurodegenerative Disorders 2nd Edition)

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19 pages, 3862 KiB

Open AccessArticle

Efficacy of Omega-5 NanoPSO Treatment in the Hippocampus, Through Antioxidant Mechanisms, After an Ischemia/Reperfusion Injury, in Murine Model

by Irene Guadalupe Aguilar-García, Jonatan Alpirez, José Francisco Muñoz-Valle, Walter Ángel Trujillo-Rangel, David Fernández-Quezada, Sergio Horacio Dueñas-Jiménez, María de la Luz Galvan-Ramírez, María Guadalupe Sánchez-Parada, Ana Elizabeth González-Santiago, Judith Marcela Dueñas-Jiménez and Rolando Castañeda-Arellano

Antioxidants 2024, 13(11), 1353; https://doi.org/10.3390/antiox13111353 - 5 Nov 2024

Abstract

Stroke is the third cause of death worldwide and a health problem, and current therapy continues to be very poor. It promotes an alteration associated with excitotoxicity, oxidative stress, and inflammatory processes, exacerbating the damage in the brain. Although cortical areas are the [...] Read more.

Stroke is the third cause of death worldwide and a health problem, and current therapy continues to be very poor. It promotes an alteration associated with excitotoxicity, oxidative stress, and inflammatory processes, exacerbating the damage in the brain. Although cortical areas are the most affected by stroke, the hippocampus can be impacted in the long term through the pathways it connects with these areas, which are associated further with motor alterations; this encourages the search for new therapeutic approaches. Omega-5, being an antioxidant, participates in regulating oxidative stress. A recently designed nanoemulsified compound coupled with pomegranate seed oil (NanoPSO) maintains bioavailability in the body for longer. Omega-5 NanoPSO is more effective in different models of neurodegenerative diseases and metabolic disorders. Therefore, it is important to analyze the effect of omega-5 NanoPSO on ischemic damage through changes in the hippocampus, oxidative mechanisms, and behavioral outcomes. Male Wistar rats were used in five groups; three groups were subjected to an ischemic event through bilateral occlusion of the carotid arteries. An ischemia group received omega-5 NanoPSO after injury, and another group received omega-5 NanoPSO performed two weeks before the ischemic event and three weeks after the surgical process. The control and sham groups did not show changes in the hippocampus and behavior. In the ischemia group, neuronal loss, oxidative stress, and a higher expression of astrocytes were maintained in the hippocampal region, and behavior was modified. In the post and pre-treatment group with omega-5 NanoPSO, we observed reduced damage, glial proliferation, and oxidative stress. It increased neuron survival in the hippocampal region and improved the locomotion. These results highlight its promise for use in clinical settings to treat patients suffering from ischemic brain injury. Full article

(This article belongs to the Special Issue New Strategies in Preventing Inflammatory and/or Oxidative-Stress-Induced Damages in Ischemia–Reperfusion Injury)

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15 pages, 6401 KiB

Open AccessArticle

Protective Mechanism of Sea buckthorn Proanthocyanidins Against Hydrogen Peroxide-Introduced Oxidative Damage in Adult Retinal Pigment Epithelial-19

by Kaiyuan Ma, Michael Yuen, Tina Yuen, Hywel Yuen and Qiang Peng

Antioxidants 2024, 13(11), 1352; https://doi.org/10.3390/antiox13111352 - 5 Nov 2024

Abstract

Retinal pigment epithelial (RPE) is an oxidation-resistant cell. But if it is subjected to various harmful stimuli for a prolonged period, an excessive amount of oxyradical will be generated to cause retinal dysfunction. We investigated and elucidated the protective mechanism of Sea buckthorn [...] Read more.

Retinal pigment epithelial (RPE) is an oxidation-resistant cell. But if it is subjected to various harmful stimuli for a prolonged period, an excessive amount of oxyradical will be generated to cause retinal dysfunction. We investigated and elucidated the protective mechanism of Sea buckthorn proanthocyanidins (SBP) against oxidative damage in RPE. In this study, we established an oxidative damage model of adult retinal pigment epithelial cell line-19 (ARPE-19) using hydrogen peroxide (H₂O₂), followed by different concentrations of SBP for 24 h. The finding demonstrated that SBP effectively inhibited the generation of malondialdehyde (MDA), restored the activity of superoxide dismutase (SOD) and content of glutathione (GSH), and significantly eliminated the level of reactive oxygen species (ROS) and oxidative stress. It was revealed that 100 µg/mL of SBP was more suitable for restoring oxidative damage in ARPE-19, which enhanced cell activity and migration ability and maintained normal cell morphology. In addition, SBP increased the expression of Bcl-2, decreased the expression of Bax and caspase-3, and activated the Nrf2/HO-1 signaling pathway to protect ARPE-19 from oxidative stress. Moreover, SBP could restore the morphology and quantity of mitochondria and inhibit mitochondrial permeability and swelling. The present results provide a theoretical basis for the protective and restorative effect of SBP in retinopathy caused by oxidative stress. Full article

(This article belongs to the Special Issue Antioxidants and Retinal Diseases—2nd Edition)

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16 pages, 2605 KiB

Open AccessArticle

Carnosine Synthase (TsATPGD) Alleviates Lipid Peroxidation Under Transcriptional Control by an Nfe2-like Gene in Tridacna Squamosa

by Zhuo Yang, Nai-Kei Wong, Fan Mao, Siwei Wu, Wenjie Yi, Ziniu Yu and Yang Zhang

Antioxidants 2024, 13(11), 1351; https://doi.org/10.3390/antiox13111351 - 4 Nov 2024

Abstract

As an important mollusk in reef ecosystems, Tridacna squamosa forms pro-survival symbiotic relationships that hinge on an exquisite redox equilibrium between the host and the photosynthetic symbiont, zooxanthellae. The exact regulatory mechanisms thereof remain poorly understood. In this study, a novel Nfe2-like transcription [...] Read more.

As an important mollusk in reef ecosystems, Tridacna squamosa forms pro-survival symbiotic relationships that hinge on an exquisite redox equilibrium between the host and the photosynthetic symbiont, zooxanthellae. The exact regulatory mechanisms thereof remain poorly understood. In this study, a novel Nfe2-like transcription factor in T. squamosa was identified and characterized with respect to its antioxidant and cytoprotective roles. Gene structure and phylogenetic analysis reveal that T. squamosa possesses a single transcription factor TsNfe2l in contrast to mammalian Nfe2l1 (Nrf1) and Nfe2l2 (Nrf2), belonging to protein members of the bZIP-NFE2 subfamily and functionally resembling the mammalian Nfe2l1. A conserved bZIP domain permits its binding to the antioxidant response element (ARE) in vitro and in HEK293T cells. Further analyses such as promoter prediction suggest that TsNfe2l target genes engage mainly in the regulation of multiple enzymes involved in antioxidation and allied pathways. Notably, TsNfe2l transcriptionally upregulates carnosine synthase (TsATPGD), which subsequently produces L-carnosine abundantly to shield cells from oxidative damage. Moreover, the blockage of TsNfe2l nucleic acid binding reduced the expression of TsATPGD and L-carnosine content in the gill, resulting in elevated lipid peroxidation. Collectively, our findings establish novel molecular insight into TsNfe2l as a critical regulator of redox homeostasis in T. squamosa through carnosine synthesis. Full article

(This article belongs to the Special Issue The Role of Oxidative Stress in Aquaculture)

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