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Applied Sciences
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Exploitation of Microbial Metabolites and Novel Approaches of Their Synthesis
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Exploitation of Microbial Metabolites and Novel Approaches of Their Synthesis

A special issue of Applied Sciences (ISSN 2076-3417). This special issue belongs to the section "Food Science and Technology".

Deadline for manuscript submissions: closed (30 April 2024) | Viewed by 2916

Special Issue Editor

Dr. Agata Urszula Fabiszewska

E-Mail Website
Guest Editor
Institute of Food Sciences, Warsaw University of Life Sciences, 159c Nowoursynowska St., 02-776 Warsaw, Poland
Interests: lactic acid bacteria; silage microbiology; yeast biotechnology; circular economy, waste management
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Microbial metabolites such as antibiotics, pigments, aroma compounds, hormones, drug agents, antimicrobials, antioxidants and enzymes are of great interest due to their huge potential for human life and health. Among the microorganisms producing the above-mentioned compounds, there can be enumerated archeabacteria, bacteria, yeast, molds and algae. Bioactive compounds have their action in small amounts and are still being investigated in medicine, chemistry, food technology and ecology. We hardly know a small percentage of all organisms living on earth. Moreover, we search for so-called extremophiles. These microorganisms can, in contrast to mesophiles, grow under extreme conditions. It can be imagined that such organisms either produce a different range of enzymes than mesophiles, or produce enzymes with extreme characteristics, such as temperature or stability and activity at extreme pH values. Exploiting new microorganisms is not the only way of possessing new metabolites; recombinant DNA techniques may open the door to the application of microbial bioactive compounds. Still, introducing new bioprocessing techniques can be a promising idea to synthesize with higher yield microbial-derived biologically active molecular entities.

This Special Issue welcomes papers addressing the different effects that exposure studies on new microbial metabolites, metabolites already known from newly discovered or engineered cells or microbial metabolites with improved yield by novel methodology of culture.

Dr. Agata Urszula Fabiszewska
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Applied Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • microbial metabolites
  • genetic manipulation
  • batch culture
  • novel resources
  • green chemistry

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Published Papers (2 papers)

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Research

21 pages, 800 KiB  
Article
Comparison of the Chemical and Aroma Composition of Low-Alcohol Beers Produced by Saccharomyces cerevisiae var. chevalieri and Different Mashing Profiles
by Aneta Pater, Magdalena Januszek and Paweł Satora
Appl. Sci. 2024, 14(12), 4979; https://doi.org/10.3390/app14124979 - 7 Jun 2024
Viewed by 815
Abstract
Changing consumer preferences and increasing demands require adjustments in brewery operations and beer production methods. Recent trends indicate a marked decline in interest in high-alcohol beers and an increasing demand for low- and no-alcohol alternatives. The aim of this study was to evaluate [...] Read more.
Changing consumer preferences and increasing demands require adjustments in brewery operations and beer production methods. Recent trends indicate a marked decline in interest in high-alcohol beers and an increasing demand for low- and no-alcohol alternatives. The aim of this study was to evaluate and compare the volatile compound profiles produced by Saccharomyces cerevisiae var. chevalieri, a yeast strain specifically developed for non-alcoholic beer production, with a reference sample fermented with a standard Saccharomyces cerevisiae US-05 strain. Two mashing profiles were compared (with and without saccharification pause). The wort obtained was fermented with and without hops. The chemical composition and aroma compounds of the resulting beers were analysed using different chromatographic techniques (HPLC, GC-FID, GC-MS and CG-O). The modification of the mashing profile helped to obtain wort with about 50% lower maltose content. A lower FAN (free amino nitrogen) content was also observed, but this did not affect the fermentation process. Beers fermented with the Saccharomyces cerevisiae var. chevalieri strain had an average alcohol content of 0.5–0.8% v/v. This strain consumed about 25% of the available maltose. The resulting beers were dominated by fruity, floral and herbal aromas. In addition, beers fermented with a non-alcoholic beer strain scored highest in the sensory analysis. Full article
(This article belongs to the Special Issue Exploitation of Microbial Metabolites and Novel Approaches of Their Synthesis)
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15 pages, 4346 KiB  
Article
Unconventional Extraction Methods of Oleaginous Yeast Cell Pretreatment and Disruption
by Agata Fabiszewska, Anna Pakulska, Bartłomiej Zieniuk, Katarzyna Wierzchowska, Karina Jasińska, Jolanta Małajowicz and Dorota Nowak
Appl. Sci. 2023, 13(24), 13135; https://doi.org/10.3390/app132413135 - 10 Dec 2023
Cited by 3 | Viewed by 1712
Abstract
Extraction is one of the most commonly used methods for obtaining and purifying chemical compounds for commercial usage. The aim of this study was to evaluate the effect of unconventional permeabilization and cell disruption methods on the yield of lipid extraction from cells [...] Read more.
Extraction is one of the most commonly used methods for obtaining and purifying chemical compounds for commercial usage. The aim of this study was to evaluate the effect of unconventional permeabilization and cell disruption methods on the yield of lipid extraction from cells of the oleaginous yeast Yarrowia lipolytica. Batch cultures in a medium with molasses and waste post-frying oil were carried out. The biomass was subjected to pulsed electric field (PEF), high-pressure processing (HPP), ultrasounds (US), and several conventional processing techniques with chemical and mechanical agents (glass beads, acetone, Triton and Tween surfactants). The effectiveness of the applied methods, either on cell permeabilization or cell disruption, was investigated by analyzing the oil and total protein extraction yield and oil leaching efficiency, as well as by using microscope images. The PEF and US treatments proved to be effective permeabilization methods as a step of sample pretreatment for extraction. These unconventional physical methods could efficiently increase intracellular lipid extraction yield in solvent applications. Full article
(This article belongs to the Special Issue Exploitation of Microbial Metabolites and Novel Approaches of Their Synthesis)
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