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Bioengineering
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Tissue Engineering and Regenerative Medicine in Bioengineering
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Tissue Engineering and Regenerative Medicine in Bioengineering

A special issue of Bioengineering (ISSN 2306-5354). This special issue belongs to the section "Regenerative Engineering".

Deadline for manuscript submissions: 31 January 2025 | Viewed by 6976

Special Issue Editors

Prof. Dr. Lynn L.H. Huang

E-Mail Website
Guest Editor
Institute of Biotechnology, College of Bioscience and Biotechnology, National Cheng Kung University, Tainan, Taiwan
Interests: biomaterials and tissue engineering
Dr. Chun-Yen Liu

E-Mail Website
Guest Editor
Department of Materials Science & Engineering, National Cheng Kung University, Tainan 70101, Taiwan
Interests: tissue engineering; biomaterials; liquid crystal material; soft material; functional polymers
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

The human body has the natural ability to heal itself in many ways. It may take a few days to a few months or even a year for the tissue to recover based on the degree of damage. Because patients may feel uncomfortable and unpleasant during the recovery process or tissues may be unable to recover back to their original status because of seriously injury, tissue engineering materials and regenerative medicine play an important role for both medical applications and academic research fields, which has resulted in these materials raising a lot of attention over the past few decades.

Regenerative medicine is a broad field that includes tissue engineering, which is composed of three important factors: cells, scaffolds, and growth factors. With the help of tissue engineered materials, a variety of functionalized constructs can be achieved to restore, improve, assist, or even replace the biological functions of tissues or organs of patients. By combining different kinds of materials, regardless of natural or synthetic polymers, tissue engineered materials and regenerative materials possessing their own mechanical and physical properties can be achieved. With the rapid development of technology, biocompatible and biodegradable materials can be expected to be applied in medical and regenerative applications such as wound healing dressing, easing pain relief patch, drug delivery systems, cancer therapy, etc.

In this Special Issue, we focus on tissue engineering and regenerative medical materials used in the field of cellular and/or material–cellular interactions for tissue engineering and regenerative medicine. Related original articles, communications, and reviews are all welcome in this Special Issue.

Prof. Dr. Lynn L.H. Huang
Dr. Chun-Yen Liu
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Bioengineering is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

 

Keywords

  • tissue engineering
  • regenerative material
  • biomedical material
  • medical material
  • polymeric material
  • synthetic material
  • natural derivative material
  • biopolymer
  • soft material

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Published Papers (4 papers)

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Research

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15 pages, 5966 KiB  
Article
Long-Term Survival and Regeneration Following Transplantation of 3D-Printed Biodegradable PCL Tracheal Grafts in Large-Scale Porcine Models
by Sen-Ei Shai, Yi-Ling Lai, Yi-Wen Hung, Chi-Wei Hsieh, Kuo-Chih Su, Chun-Hsiang Wang, Te-Hsin Chao, Yung-Tsung Chiu, Chia-Ching Wu and Shih-Chieh Hung
Bioengineering 2024, 11(8), 832; https://doi.org/10.3390/bioengineering11080832 - 14 Aug 2024
Viewed by 932
Abstract
Polycaprolactone (PCL) implants in large animals show great promise for tracheal transplantation. However, the longest survival time achieved to date is only about three weeks. To meet clinical application standards, it is essential to extend the survival time and ensure the complete integration [...] Read more.
Polycaprolactone (PCL) implants in large animals show great promise for tracheal transplantation. However, the longest survival time achieved to date is only about three weeks. To meet clinical application standards, it is essential to extend the survival time and ensure the complete integration and functionality of the implant. Our study investigates the use of three-dimensional (3D)-printed, biodegradable, PCL-based tracheal grafts for large-scale porcine tracheal transplantation, assessing the feasibility and early structural integrity crucial for long-term survival experiments. A biodegradable PCL tracheal graft was fabricated using a BIOX bioprinter and transplanted into large-scale porcine models. The grafts, measuring 20 × 20 × 1.5 mm, were implanted following a 2 cm circumferential resection of the porcine trachea. The experiment design was traditionally implanted in eight porcines to replace four-ring tracheal segments, only two of which survived more than three months. Data were collected on the graft construction and clinical outcomes. The 3D-printed biosynthetic grafts replicated the native organ with high fidelity. The implantations were successful, without immediate complications. At two weeks, bronchoscopy revealed significant granulation tissue around the anastomosis, which was managed with laser ablation. The presence of neocartilage, neoglands, and partial epithelialization near the anastomosis was verified in the final pathology findings. Our study demonstrates in situ regenerative tissue growth with intact cartilage following transplantation, marked by neotissue formation on the graft’s exterior. The 90-day survival milestone was achieved due to innovative surgical strategies, reinforced with strap muscle attached to the distal trachea. Further improvements in graft design and granulation tissue management are essential to optimize outcomes. Full article
(This article belongs to the Special Issue Tissue Engineering and Regenerative Medicine in Bioengineering)
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19 pages, 4401 KiB  
Article
Modulation of Canine Adipose-Derived Mesenchymal Stem/Medicinal Signalling Cells with Ascorbic Acid: Effect on Proliferation and Chondrogenic Differentiation on Standard Plastic and Silk Fibroin Surfaces
by Metka Voga
Bioengineering 2024, 11(5), 513; https://doi.org/10.3390/bioengineering11050513 - 20 May 2024
Viewed by 886
Abstract
Ascorbic acid (AA) plays a crucial role in both the proliferation and chondrogenic differentiation potential of mesenchymal stem/medicinal signalling cells (MSCs); these are both key aspects of their general therapeutic use and their increasing use in veterinary medicine. Current immunomodulatory therapies require efficient [...] Read more.
Ascorbic acid (AA) plays a crucial role in both the proliferation and chondrogenic differentiation potential of mesenchymal stem/medicinal signalling cells (MSCs); these are both key aspects of their general therapeutic use and their increasing use in veterinary medicine. Current immunomodulatory therapies require efficient expansion of MSCs in the laboratory, while emerging tissue regeneration strategies, such as cartilage or bone repair, aim to use differentiated MSCs and modulate the expression of chondrogenic and hypertrophic markers. Our aim was to investigate whether the addition of AA to the growth medium enhances the proliferation of canine adipose-derived MSCs (cAMSCs) grown on standard plastic surfaces and whether it affects chondrogenic differentiation potential on silk fibroin (SF) films. We assessed cell viability with trypan blue and proliferation potential by calculating population doubling. Chondrogenic induction on SF films was assessed by Alcian blue staining and gene expression analysis of chondrogenic and hypertrophic genes. The results showed that growth medium with AA significantly enhanced the proliferation of cAMSCs without affecting cell viability and modulated the expression of chondrogenic and hypertrophic genes of cAMSCs grown on SF films. Our results suggest that AA may be used in growth medium for expansion of cAMSCs and, at the same time, provide the basis for future studies to investigate the role of AA and SF in chondrogenic differentiation of MSCs. Full article
(This article belongs to the Special Issue Tissue Engineering and Regenerative Medicine in Bioengineering)
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16 pages, 11527 KiB  
Article
Perfusable Tissue Bioprinted into a 3D-Printed Tailored Bioreactor System
by Marius Gensler, Christoph Malkmus, Philipp Ockermann, Marc Möllmann, Lukas Hahn, Sahar Salehi, Robert Luxenhofer, Aldo R. Boccaccini and Jan Hansmann
Bioengineering 2024, 11(1), 68; https://doi.org/10.3390/bioengineering11010068 - 9 Jan 2024
Cited by 1 | Viewed by 2868
Abstract
Bioprinting provides a powerful tool for regenerative medicine, as it allows tissue construction with a patient’s specific geometry. However, tissue culture and maturation, commonly supported by dynamic bioreactors, are needed. We designed a workflow that creates an implant-specific bioreactor system, which is easily [...] Read more.
Bioprinting provides a powerful tool for regenerative medicine, as it allows tissue construction with a patient’s specific geometry. However, tissue culture and maturation, commonly supported by dynamic bioreactors, are needed. We designed a workflow that creates an implant-specific bioreactor system, which is easily producible and customizable and supports cell cultivation and tissue maturation. First, a bioreactor was designed and different tissue geometries were simulated regarding shear stress and nutrient distribution to match cell culture requirements. These tissues were then directly bioprinted into the 3D-printed bioreactor. To prove the ability of cell maintenance, C2C12 cells in two bioinks were printed into the system and successfully cultured for two weeks. Next, human mesenchymal stem cells (hMSCs) were successfully differentiated toward an adipocyte lineage. As the last step of the presented strategy, we developed a prototype of an automated mobile docking station for the bioreactor. Overall, we present an open-source bioreactor system that is adaptable to a wound-specific geometry and allows cell culture and differentiation. This interdisciplinary roadmap is intended to close the gap between the lab and clinic and to integrate novel 3D-printing technologies for regenerative medicine. Full article
(This article belongs to the Special Issue Tissue Engineering and Regenerative Medicine in Bioengineering)
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Review

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17 pages, 1149 KiB  
Review
Regulation of Oxygen Tension as a Strategy to Control Chondrocytic Phenotype for Cartilage Tissue Engineering and Regeneration
by Mikko J. Lammi and Chengjuan Qu
Bioengineering 2024, 11(3), 211; https://doi.org/10.3390/bioengineering11030211 - 23 Feb 2024
Cited by 1 | Viewed by 1659
Abstract
Cartilage defects and osteoarthritis are health problems which are major burdens on health care systems globally, especially in aging populations. Cartilage is a vulnerable tissue, which generally faces a progressive degenerative process when injured. This makes it the 11th most common cause of [...] Read more.
Cartilage defects and osteoarthritis are health problems which are major burdens on health care systems globally, especially in aging populations. Cartilage is a vulnerable tissue, which generally faces a progressive degenerative process when injured. This makes it the 11th most common cause of global disability. Conservative methods are used to treat the initial phases of the illness, while orthopedic management is the method used for more progressed phases. These include, for instance, arthroscopic shaving, microfracturing and mosaicplasty, and joint replacement as the final treatment. Cell-based implantation methods have also been developed. Despite reports of successful treatments, they often suffer from the non-optimal nature of chondrocyte phenotype in the repair tissue. Thus, improved strategies to control the phenotype of the regenerating cells are needed. Avascular tissue cartilage relies on diffusion for nutrients acquisition and the removal of metabolic waste products. A low oxygen content is also present in cartilage, and the chondrocytes are, in fact, well adapted to it. Therefore, this raises an idea that the regulation of oxygen tension could be a strategy to control the chondrocyte phenotype expression, important in cartilage tissue for regenerative purposes. This narrative review discusses the aspects related to oxygen tension in the metabolism and regulation of articular and growth plate chondrocytes and progenitor cell phenotypes, and the role of some microenvironmental factors as regulators of chondrocytes. Full article
(This article belongs to the Special Issue Tissue Engineering and Regenerative Medicine in Bioengineering)
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