Advances in Foodborne Pathogens

A special issue of Biology (ISSN 2079-7737). This special issue belongs to the section "Microbiology".

Deadline for manuscript submissions: 31 July 2026 | Viewed by 3725

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School of Life Sciences, Shanghai University, Shanghai 200444, China
Interests: biosensors; functional nucleic acids; visual detection
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Special Issue Information

Dear Colleagues,

According to the data of World Health Organization, at least 1 in 10 of the global population becomes unwell because of contaminated food and about 42,0000 people die every year. Food safety is very important for human health. Among the risk factors that threaten food safety, foodborne pathogens cause most food safety problems and consistently attract significant attention. For foodborne pathogens, we want to obtain the answers to the following questions: how do foodborne pathogens contaminate food? How do foodborne pathogens grow and reproduce in food? How can we kill foodborne pathogens or control contamination? How do foodborne pathogens infect people? How can we develop rapid, sensitive and reliable assays for the development of foodborne pathogens? The results of these questions can help people to reduce the serious consequences caused by foodborne pathogens and improve food safety.

Therefore, the Special Issue “Advances in Foodborne Pathogens” has been launched in Biology , and we are pleased to invite you to published novel studies  (articles or reviews) about the recent advances in foodborne pathogens on topics including but not limited to contamination control, risk assessment, virulence mechanism, antibacterial agents or materials, and detection assays.

Dr. Qiming Chen
Guest Editor

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Keywords

  • foodborne pathogens
  • contamination mechanisms
  • infection mechanisms
  • detection assays
  • risk assessment
  • antibacterial agents or materials

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Published Papers (3 papers)

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Research

32 pages, 7169 KB  
Article
Phage Frontiers: Genomic and Functional Profiling of Novel Virulent Agents Targeting Foodborne Enterobacteriaceae
by Ramy Abdelreheim Qabel, Miao Xu, Chunwen Li, Chuhan Zhang, Chuanzhi Zhang, Yong Huang, Guangming Xiong, Edmund Maser and Liquan Guo
Biology 2026, 15(7), 578; https://doi.org/10.3390/biology15070578 - 4 Apr 2026
Viewed by 697
Abstract
Foodborne pathogens of Enterobacteriaceae are becoming an increasing global concern, with multidrug-resistant strains posing significant risks to food safety and public health, especially in high-risk products like dairy. This research focused on isolating, biologically characterizing, and genomically profiling new bacteriophages that target key [...] Read more.
Foodborne pathogens of Enterobacteriaceae are becoming an increasing global concern, with multidrug-resistant strains posing significant risks to food safety and public health, especially in high-risk products like dairy. This research focused on isolating, biologically characterizing, and genomically profiling new bacteriophages that target key Enterobacteriaceae members as potential biocontrol agents. Eight phages were isolated from wastewater using four bacterial hosts and analyzed through transmission electron microscopy, one-step growth analysis, adsorption kinetics, host range evaluation, whole-genome sequencing, comparative genomics, phylogenetic analysis, proteomic profiling, and virion assembly pathway characterization. All eight isolates exhibited icosahedral heads with contractile tails typical of Myoviridae morphology, demonstrated broad-spectrum lytic activity against 21 bacterial strains (infectivity: 47.6–95.2%), showed high adsorption efficiencies (84.75–99.98%), and had burst sizes ranging from 11 to 166 particles per cell. Genome sizes varied from 103 to 170 kb with coding densities between 92–96%. Importantly, none contained antimicrobial resistance genes, virulence factors, or lysogeny-associated elements, confirming their strictly lytic lifestyles and favorable biosafety profiles. Phylogenetic and comparative analyses indicated mosaic genomic structures influenced by horizontal gene transfer rather than host phylogeny. These findings provide a robust biological and genomic basis for evaluating these phages as potentially safe and effective alternatives to antibiotics in controlling foodborne Enterobacteriaceae, pending further in situ validation. Full article
(This article belongs to the Special Issue Advances in Foodborne Pathogens)
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25 pages, 2007 KB  
Article
Molecular Profiling of Foodborne Pathogens in Ready-to-Eat Foods, Al-Madinah Al-Munawarah, Saudi Arabia
by Omar Almutairi, Ihab M. Moussa, Eman Marzouk, Adil Abalkhail and Ayman Elbehiry
Biology 2026, 15(1), 104; https://doi.org/10.3390/biology15010104 - 5 Jan 2026
Cited by 2 | Viewed by 1120
Abstract
Foodborne pathogens remain a global public health concern, and antimicrobial resistance increases their impact. In mass-gathering cities such as Al-Madinah Al-Munawarah, contaminated ready-to-eat (RTE) fast foods can contribute to both local transmission and international spread. In this study, 300 RTE fast food samples, [...] Read more.
Foodborne pathogens remain a global public health concern, and antimicrobial resistance increases their impact. In mass-gathering cities such as Al-Madinah Al-Munawarah, contaminated ready-to-eat (RTE) fast foods can contribute to both local transmission and international spread. In this study, 300 RTE fast food samples, including shawarma, burgers, fried chicken, sandwiches, and salads, were collected from international franchises, local restaurants, and street vendors. Pathogens were identified using conventional culture combined with matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). Antimicrobial susceptibility testing followed CLSI guidelines, and real-time PCR confirmed species identity and screened resistance determinants. Principal component analysis (PCA) and dendrogram clustering were used to assess diagnostic discrimination. Among the 300 samples, 129 (43.0%) were culture positive. The most common pathogens were Staphylococcus aureus (14.3%) and Escherichia coli (13.0%), followed by Salmonella spp. (9.0%) and Acinetobacter baumannii (6.7%). About 35% of S. aureus isolates were methicillin resistant (MRSA), and 85% of A. baumannii carried OXA-type carbapenemase genes. MALDI-TOF MS achieved 96.1% score-based identification and, with PCA, showed strong interspecies separation. PCR confirmed species identity and detected widespread resistance genes, with genotype–phenotype concordance of at least 80%. Overall, 60.5% of isolates were multidrug resistant. RTE fast foods in Al-Madinah represent reservoirs of MDR pathogens, including carbapenemase-producing A. baumannii. The combined use of MALDI-TOF MS and real-time PCR established a rapid and scalable workflow that provided reliable identification and resistance profiling in less than 24 h, compared with 48 to 72 h for conventional methods. This approach supports One Health surveillance in high-risk food settings and strengthens preparedness for mass gatherings. Full article
(This article belongs to the Special Issue Advances in Foodborne Pathogens)
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10 pages, 946 KB  
Article
Development of a Visual Assay for Detection of Viable Cronobacter sakazakii Using RT-PSR and Hydroxynaphthol Blue Indicator
by Peng Wang, Qiming Chen, Yikai Wang, Xueting Sun and Zhanmin Liu
Biology 2025, 14(4), 383; https://doi.org/10.3390/biology14040383 - 7 Apr 2025
Cited by 3 | Viewed by 1150
Abstract
Cronobacter sakazakii is a foodborne pathogen in powdered infant formula, which poses a significant risk to susceptible populations such as infants and the elderly. This study aims to develop a visual detection method for viable C. sakazakii using the reverse transcription-polymerase spiral reaction [...] Read more.
Cronobacter sakazakii is a foodborne pathogen in powdered infant formula, which poses a significant risk to susceptible populations such as infants and the elderly. This study aims to develop a visual detection method for viable C. sakazakii using the reverse transcription-polymerase spiral reaction and hydroxynaphthol blue indicator. Under the optimized conditions, the detection process could be completed within 55 min with low equipment dependence. It was evaluated to have high specificity and sensitivity with the detection limit low to 1.2 × 101 CFU/mL. The assay also showed 100% accuracy in artificially contaminated samples. Full article
(This article belongs to the Special Issue Advances in Foodborne Pathogens)
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