Innovative Approaches in In Vitro Skin Models: From Design to Application

A special issue of Biomedicines (ISSN 2227-9059). This special issue belongs to the section "Biomedical Engineering and Materials".

Deadline for manuscript submissions: 28 February 2025 | Viewed by 281

Special Issue Editor

1. The Institute of Biomedical Sciences, Faculty of Medicine, University of Maribor, Taborska ulica 8, 2000 Maribor, Slovenia
2. The Department of Pharmacology, Faculty of Medicine, University of Maribor, Taborska ulica 8, 2000 Maribor, Slovenia
Interests: in vitro skin model; 3D bioprinting; tissue engineering; wound dressings; electrospinning
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Special Issue Information

Dear Colleagues,

We are pleased to invite you to contribute to this forthcoming Special Issue of Biomedicines, aiming to showcase the latest advancements, novel methodologies and emerging trends in the development and application of in vitro skin models.

As you are aware, in vitro skin models have become invaluable tools in dermatological research, cosmetic testing and the study of skin diseases. These models offer significant potential for reducing reliance on animal testing, enhancing our understanding of skin physiology and accelerating the development of new therapeutic strategies. However, despite their widespread use, there remain challenges in replicating the complexity and diversity of human skin.

This Special Issue seeks to highlight innovative approaches that address these challenges, including, but not limited to, the following:

  • Novel designs and fabrication techniques for in vitro skin models;
  • Advanced materials and bioengineering approaches for skin model construction;
  • Integration of multi-cellular components to better mimic skin physiology;
  • Applications of in vitro skin models in drug testing, disease modeling and cosmetic evaluation;
  • Challenges and future directions in the field of in vitro skin models.

We welcome original research articles, reviews, case studies and short communications that contribute to these themes. We are particularly interested in submissions that provide new insights into the design, development and application of in vitro skin models.

We look forward to your submission.

Dr. Tina Maver
Guest Editor

Manuscript Submission Information

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Keywords

  • in vitro skin models
  • tissue engineering
  • dermatological research
  • 3D skin bioprinting
  • skin disease modeling

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Published Papers (1 paper)

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Research

19 pages, 5049 KiB  
Article
The Impact of Temperature and the Duration of Freezing on a Hydrogel Used for a 3D-Bioprinted In Vitro Skin Model
by Maja Sever, Dominik Škrinjar, Tina Maver, Monika Belak, Franc Zupanič, Ivan Anžel and Tanja Zidarič
Biomedicines 2024, 12(9), 2028; https://doi.org/10.3390/biomedicines12092028 - 5 Sep 2024
Viewed by 188
Abstract
Skin bioprinting has the potential to revolutionize treatment approaches for injuries and surgical procedures, while also providing a valuable platform for assessing and screening cosmetic and pharmaceutical products. This technology offers key advantages, including flexibility and reproducibility, which enable the creation of complex, [...] Read more.
Skin bioprinting has the potential to revolutionize treatment approaches for injuries and surgical procedures, while also providing a valuable platform for assessing and screening cosmetic and pharmaceutical products. This technology offers key advantages, including flexibility and reproducibility, which enable the creation of complex, multilayered scaffolds that closely mimic the intricate microenvironment of native skin tissue. The development of an ideal hydrogel is critical for the successful bioprinting of these scaffolds with incorporated cells. In this study, we used a hydrogel formulation developed in our laboratory to fabricate a 3D-bioprinted skin model. The hydrogel composition was carefully selected based on its high compatibility with human skin cells, incorporating alginate, methyl cellulose, and nanofibrillated cellulose. One of the critical challenges in this process, particularly for its commercialization and large-scale production, is ensuring consistency with minimal batch-to-batch variations. To address this, we explored methods with which to preserve the physicochemical properties of the hydrogels, with a focus on freezing techniques. We validated the pre-frozen hydrogels’ printability, rheology, and mechanical and surface properties. Our results revealed that extended freezing times significantly reduced the viscosity of the formulations due to ice crystal formation, leading to a redistribution of the polymer chains. This reduction in viscosity resulted in a more challenging extrusion and increased macro- and microporosity of the hydrogels, as confirmed by nanoCT imaging. The increased porosity led to greater water uptake, swelling, compromised scaffold integrity, and altered degradation kinetics. The insights gained from this study lay a solid foundation for advancing the development of an in vitro skin model with promising applications in preclinical and clinical research. Full article
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