Virulence and Antimicrobial Resistance: Convergence or Divergence

A special issue of Biomedicines (ISSN 2227-9059). This special issue belongs to the section "Microbiology in Human Health and Disease".

Deadline for manuscript submissions: closed (31 August 2023) | Viewed by 1639

Special Issue Editors


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Guest Editor
Pediatric Research and Clinical Center for Infectious Diseases, Saint Petersburg, Russia
Interests: antimicrobial resistance; carbapenemases; MRSA; bacterial genomics

E-Mail Website
Guest Editor
Pediatric Research and Clinical Center for Infectious Diseases, Saint Petersburg, Russia
Interests: emerging infectious diseases; infectious disease epidemiology; infectious disease control and prevention; microbiology; molecular biology; epidemiology; PCR; infectious diseases; antibiotic resistance; antimicrobials

Special Issue Information

Dear Colleagues,

Antimicrobial resistance and virulence are among the most important properties of bacteria in practice, but traditionally they were considered by microbiologists as relatively independent phenomena. Even the results of research in these areas have been published in various journals. The situation changed dramatically after the appearance of genetic lines of Klebsiella possessing both the properties of multiple resistance and increased virulence. Today, this phenomenon is considered one of the global and serious threats to the health care system. The number of works on the problem is growing exponentially; a number of convergence mechanisms have already been described, but progress in the development of treatment methods is very small. Obviously, to solve this problem, a deeper understanding of evolution and the molecular mechanisms of convergence is required, which in turn requires fundamental research involving systems biology methods. Although the phenomenon of convergence has been found in Klebsiella, it is likely that its formation and distribution is not limited to this species. This Special Issue of the multidisciplinary journal Biomedicines provides a unique platform for researchers from various fields to join forces to curb convergence and virulence.

Dr. Sergey Sidorenko
Dr. Vladimir Ageevets
Guest Editors

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Keywords

  • antimicrobial resistance
  • virulence
  • convergence
  • gram-negative bacteria
  • Klebsiella

Published Papers (1 paper)

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Research

13 pages, 4387 KiB  
Article
Metabolic and Phenotypic Changes Induced during N-Acetylglucosamine Signalling in the Fungal Pathogen Candida albicans
by Somnath Sahoo, Sarika Sharma, Mahendra P. Singh, Sandeep K. Singh, Emanuel Vamanu and Kongara Hanumantha Rao
Biomedicines 2023, 11(7), 1997; https://doi.org/10.3390/biomedicines11071997 - 14 Jul 2023
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Abstract
The human commensal yeast Candida albicans is pathogenic and results in a variety of mucosal and deep tissue problems when the host is immunocompromised. Candida exhibits enormous metabolic flexibility and dynamic morphogenetic transition to survive under host niche environmental conditions and to cause [...] Read more.
The human commensal yeast Candida albicans is pathogenic and results in a variety of mucosal and deep tissue problems when the host is immunocompromised. Candida exhibits enormous metabolic flexibility and dynamic morphogenetic transition to survive under host niche environmental conditions and to cause virulence. The amino sugar N-acetylglucosamine (GlcNAc) available at the host infection sites, apart from acting as an extremely good carbon and nitrogen source, also induces cellular signalling in this pathogen. In C. albicans, GlcNAc performs multifaceted roles, including GlcNAc scavenging, GlcNAc import and metabolism, morphogenetic transition (yeast—hyphae and white—opaque switch), GlcNAc-induced cell death (GICD), and virulence. Understanding the molecular mechanism(s) involved in GlcNAc-induced cellular processes has become the main focus of many studies. In the current study, we focused on GlcNAc-induced metabolic changes associated with phenotypic changes. Here, we employed gas chromatography–mass spectrometry (GC–MS), which is a high-throughput and sensitive technology, to unveil global metabolomic changes that occur in GlcNAc vs. glucose grown conditions in Candida cells. The morphogenetic transition associated with metabolic changes was analysed by high-resolution field emission scanning electron microscopy (FE-SEM). Metabolite analysis revealed the upregulation of metabolites involved in the glyoxylate pathway, oxidative metabolism, and fatty acid catabolism to probably augment the synthesis of GlcNAc-induced hypha-specific materials. Furthermore, GlcNAc-grown cells showed slightly more sensitivity to amphotericin B treatment. These results all together provide new insights into the development of antifungal therapeutics for the control of candidiasis in humans. Full article
(This article belongs to the Special Issue Virulence and Antimicrobial Resistance: Convergence or Divergence)
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