New Technology of Biomarker Detection Based on Microbial-Derived Biosensors

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensor and Bioelectronic Devices".

Deadline for manuscript submissions: closed (30 April 2023) | Viewed by 2617

Special Issue Editor


E-Mail Website
Guest Editor
State Key Laboratory of Marine Resource Utilization in South China Sea, School of Pharmaceutical Sciences, Hainan University, Haikou 570228, China
Interests: biosensors; microbiological diagnostic mechanisms
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Through millions of years of evolution, microorganisms have developed defense systems to protect them from attack by invading species (e.g., CRISPR-Cas for establishing adaptive immune systems and nanopore-forming toxins as virulence factors) or enable them to adapt to different conditions (e.g., gas vesicles for achieving buoyancy control). These microorganism defense systems (MDSs) have inspired the development of biosensors, receiving much attention in many fields ranging from life science research to food safety to medical diagnosis.

Compared to nanomaterial-based biosensors, MDS-derived next-generation biosensing methods provide excellent results with high sensitivity and specificity and, in combination with fluorescent, electrochemical, colorimetric and acoustic devices, offer an important analytical tool for the low-cost measurement of nucleic acids, protein, bacteria cells, small molecules and metal ions. The low-cost nature of these techniques is facilitated by their scalability, as the production of microorganisms, proteins or DNA can be easily scaled up to adjust to the market needs by simply using larger or more bioreactors. Paper-based detection methods are not only easy to scale up, but also cheap to store and deliver, able to be dried and preserved for long term with no need for refrigeration.

We invite research submissions helping towards advancing the field of MDS-derived next-generation biosensing and its application for the high-throughput analysis of biomarkers.

Dr. Yi Wan
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • microbe-derived biosensors
  • CRISPR-Cas system
  • nucleic acid diagnostic
  • nanopore
  • gas vesicles
  • bacteria detection

Published Papers (1 paper)

Order results
Result details
Select all
Export citation of selected articles as:

Research

11 pages, 3249 KiB  
Article
Fluorescent Probe Combined with Photoelectric Analysis Technology for Detection of Escherichia coli
by Qian Cui, Yongjie Zhong, Wenkai Shang, Fuming Deng, Buhua Wang, Jiajia Wu, Peng Wang, Liudang Wan, Keling Wang, Lingchen Fang, Rui Dai, Han Zhang, Rodrigo Ledesma-Amaro, Yunuo Zhang and Jiaomei Huang
Biosensors 2023, 13(2), 150; https://doi.org/10.3390/bios13020150 - 18 Jan 2023
Cited by 2 | Viewed by 1994
Abstract
Food safety is facing great challenges in preventing foodborne diseases caused by pathogenic pollution, especially in resource-limited areas. The rapid detection technique of microorganisms, such as immunological methods and molecular biological methods, plays a crucial key in timely bioanalysis and disease treatment strategies. [...] Read more.
Food safety is facing great challenges in preventing foodborne diseases caused by pathogenic pollution, especially in resource-limited areas. The rapid detection technique of microorganisms, such as immunological methods and molecular biological methods, plays a crucial key in timely bioanalysis and disease treatment strategies. However, it is difficult for these methods to simultaneously meet the criteria of simple operation, high specificity, and sensitivity, as well as low cost. Coconut water is known as the “water of life” in Hainan. It is a refreshing and nutritious beverage which is widely consumed due to its beneficial properties to health. Coconut water processing is an important pillar industry in Hainan. The detection of pathogenic microorganisms, such as Escherichia coli, in coconut water has become an important factor which has restricted the upgrading and development of this industry. Based on the needs of industrial development, we developed a microbial photoelectric detection system which was composed of a fluorescent probe detection reagent and a photoelectric sensor detection device. This system combined microbial enzyme targets, selective fluorescent substrate metabolism characteristics, and a photoelectric sensor signal transduction mechanism, which produce a strong signal with a high signal-to-noise ratio. The microbial detection system developed here has a simple structure, simple and convenient operation, short detecting time (≥2 h), and high sensitivity (1 CFU/mL). This system may also enable early warning and monitoring programs for other pathogenic microorganisms in order to promote the overall competitiveness of the Hainan coconut water industry. Full article
Show Figures

Figure 1

Back to TopTop