Technological Advances in Affinity-Based Immunosensor Design

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensor and Bioelectronic Devices".

Deadline for manuscript submissions: 31 March 2025 | Viewed by 885

Special Issue Editors


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Guest Editor
Department of Biomedical Science, Chang Gung University, Taipei, Taiwan
Interests: electrochemistry; 2D MXene; graphene; nanomaterials; sensor/immunosensor; photo(electro)chemistry; energy storage applications

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Guest Editor
National Institute of Materials Physics, Atomistilor Str. 405A, 077125 Magurele, Romania
Interests: development and surface characterization of electrodes for (bio)sensing DNA, proteins and other relevant biomarkers
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Special Issue Information

Dear Colleagues,

This Special Issue presents the technological advancements that are improving the field of affinity-based immunosensors to a new level. It highlights the fundamental concepts of immunosensors, which depend on the precise binding interactions between antigens and antibodies, providing high sensitivity and specificity for the detection of various analytes. The Special Issue examines the development of advanced materials, including 2D materials, carbon nanotubes, conductive polymers and nanomaterials, which significantly improve sensor performance. It also covers the integration of microfluidic systems for improved miniaturization and portability, and the evolution of label-free detection methods that simplify sensor operation and allow for real-time analysis. Enhanced signal transduction methods, including electrochemical, optical and piezoelectric approaches, are discussed for their role in increasing detection limits and accuracy. Advanced data processing and machine learning techniques are also highlighted for their potential to improve the interpretation of sensor performance. This collection of cutting-edge research underscores the significant advancements in affinity-based immunosensor technology, paving the way for improved applications in medical diagnostics, environmental monitoring and food safety.

This Special Issue aims to invite and collect research works based on recent technological advances in affinity-based immunosensors. We welcome the submissions of original research, reviews and short communication articles on this topic.

The proposed topics to be covered in this Special Issue include (but are not limited to) the following:

  • innovative materials
  • microfluidics
  • signal transduction
  • surface functionalization
  • label-free detection
  • electrochemical
  • and photoelectrochemical.

Dr. Arumugam Sangili
Dr. Victor C. Diculescu
Guest Editors

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Keywords

  • innovative materials
  • microfluidics
  • signal transduction
  • surface functionalization
  • label-free detection
  • electrochemical
  • photoelectrochemical

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Published Papers (1 paper)

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Research

13 pages, 2688 KiB  
Article
Stannous Chloride-Modified Glass Substrates for Biomolecule Immobilization: Development of Label-Free Interferometric Sensor Chips for Highly Sensitive Detection of Aflatoxin B1 in Corn
by Alexey V. Orlov, Maria O. Zolotova, Denis O. Novichikhin, Nikolai A. Belyakov, Svetlana G. Protasova, Petr I. Nikitin and Artem V. Sinolits
Biosensors 2024, 14(11), 531; https://doi.org/10.3390/bios14110531 - 3 Nov 2024
Viewed by 714
Abstract
This study presents the development of stannous chloride (SnCl2)-modified glass substrates for biomolecule immobilization and their application in fabricating sensor chips for label-free interferometric biosensors. The glass modification process was optimized, identifying a 5% SnCl2 concentration, a 45 min reaction [...] Read more.
This study presents the development of stannous chloride (SnCl2)-modified glass substrates for biomolecule immobilization and their application in fabricating sensor chips for label-free interferometric biosensors. The glass modification process was optimized, identifying a 5% SnCl2 concentration, a 45 min reaction time, and a 150 °C drying temperature as conditions for efficient protein immobilization. Based on the SnCl2-modified glass substrates and label-free spectral-phase interferometry, a biosensor was developed for the detection of aflatoxin B1 (AFB1)—a highly toxic and carcinogenic contaminant in agricultural products. The biosensor realizes a competitive immunoassay of a remarkable detection limit as low as 26 pg/mL of AFB1, and a five-order dynamic range. The biosensor performance was validated using real corn flour samples contaminated with Aspergillus flavus. The proposed approach not only provides a powerful tool for AFB1 detection for food safety monitoring but also demonstrates the potential of SnCl2-modified substrates as a versatile platform for the development of next-generation biosensors. Full article
(This article belongs to the Special Issue Technological Advances in Affinity-Based Immunosensor Design)
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