Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
6.8
3.8
Journals
Catalysts
Special Issues
Biotransformation Catalyzed by Immobilized Enzyme
share announcement

Biotransformation Catalyzed by Immobilized Enzyme

A special issue of Catalysts (ISSN 2073-4344). This special issue belongs to the section "Biocatalysis".

Deadline for manuscript submissions: closed (30 January 2022) | Viewed by 8975

Special Issue Editors

Prof. Dr. Ulf Hanefeld

E-Mail Website
Guest Editor
Biocatalysis & Organic Chemistry, Delft University of Technology, Delft, The Netherlands
Interests: enzymes that enable the formation of C–C bonds or catalyze reactions that are chemically extremely difficult to perform; their immobilization and application in organic synthesis
Dr. Yann P. Guiavarc'h

E-Mail Website
Guest Editor
1. Biological & Food Engineering, University of Lorraine (UL), 54000 Nancy, France
2. The Reactions and Chemical Engineering Laboratory (LRGP), National Center for Scientific Research, 75016 Paris, France
Interests: enzymes, in particular aminoacylases for performing N-acylation reactions in aqueous phase, and lipases for performing O-acylation reactions in anhydrous solvents (supercritical CO2 or organic solvent)

Special Issue Information

Dear Colleagues,

It is our pleasure to invite you to contribute to this Special Issue of Catalysts, entitled “Biotransformations Catalyzed by Immobilized Enzymes”.

Biotransformations catalyzed by immobilized enzymes represent a fascinating research and development area. From studies in the laboratory via pilot scale-up to successfully implemented large- or very large-scale production in food, pharmaceutical, biofuel, and biodegradable plastics, immobilized enzymes catalyze a broad range of reactions.

Regardless of the immobilization strategy used, the targeted advantages of immobilization are:

(i)  Easy recovery/separation of the biocatalyst from the reaction mixture;

(ii)  Easy recycling through multiple runs;

(iii)  Improved stability (and sometimes activity) versus process parameters, such as temperature or pH;

(iv) Continuous flow reactors such as packed bed reactors in order to suppress inhibition of the enzyme by the reaction product(s) while getting full conversion and pure products in cases of equimolar ratio of substrates and where appropriate process conditions are used.

Combining these four advantages, region-, chemo- or enantio-selectivity of the immobilized enzymes can lead to single successful transformation or, even better, cascade biotransformation processes.

We therefore invite you to submit your current work in this area, but also in the adjacent fields such as:

(i)  Biotransformations catalyzed by immobilized enzymes in anhydrous solvent (organic, supercritical, eutectic, ionic);

(ii)  Use and up-scalability in a reactor and/or recyclability;

(iii) Process intensification involving immobilized enzymes;

(iv) Production cost and eco-compatibility (lifecycle assessments, etc.).

In this Special Issue, we welcome contributions from all aspects of biotransformations catalyzed by immobilized enzymes. The following keywords are a guideline: enzyme immobilization, enzyme recycling, enzyme carrier interaction, continuous reactions, biocatalytic cascades, non-aqueous reaction systems, downstream processing, lifecycle assessment.

We look forward to your contributions to this interesting field.

Prof. Dr. Ulf Hanefeld
Dr. Yann P. Guiavarc'h
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Catalysts is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2200 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Enzyme immobilization
  • Enzyme recycling
  • Enzyme carrier interaction
  • Continuous reactions
  • Biocatalytic cascades
  • Non-aqueous reaction systems
  • Downstream processing
  • Lifecycle assessment

Benefits of Publishing in a Special Issue

  • Ease of navigation: Grouping papers by topic helps scholars navigate broad scope journals more efficiently.
  • Greater discoverability: Special Issues support the reach and impact of scientific research. Articles in Special Issues are more discoverable and cited more frequently.
  • Expansion of research network: Special Issues facilitate connections among authors, fostering scientific collaborations.
  • External promotion: Articles in Special Issues are often promoted through the journal's social media, increasing their visibility.
  • e-Book format: Special Issues with more than 10 articles can be published as dedicated e-books, ensuring wide and rapid dissemination.

Further information on MDPI's Special Issue polices can be found here.

Published Papers (3 papers)

Download All Papers
Order results
Result details
Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:

Research

23 pages, 3925 KiB  
Article
The Effect of pH, Metal Ions, and Insoluble Solids on the Production of Fumarate and Malate by Rhizopus delemar in the Presence of CaCO3
by Dominic Kibet Ronoh, Reuben Marc Swart, Willie Nicol and Hendrik Brink
Catalysts 2022, 12(3), 263; https://doi.org/10.3390/catal12030263 - 25 Feb 2022
Cited by 4 | Viewed by 2510
Abstract
Calcium carbonate has been extensively used as a neutralising agent in acid-forming microbial processes. The effect of increasing calcium carbonate concentrations on Rhizopus delemar has not been previously investigated. In this study, an evaluation of fumaric acid (FA) and malic acid (MA) production [...] Read more.
Calcium carbonate has been extensively used as a neutralising agent in acid-forming microbial processes. The effect of increasing calcium carbonate concentrations on Rhizopus delemar has not been previously investigated. In this study, an evaluation of fumaric acid (FA) and malic acid (MA) production was conducted at three CaCO3 concentrations in shake flask cultivations. Increased CaCO3 concentrations resulted in the co-production of FA and MA in the first 55 h of the fermentation (regime 1), and the subsequent depletion of FA thereafter (regime 2). Three factors were highlighted as likely causes of this response: insoluble solids, metal ion concentrations, and pH. Further shake flask cultivations as well as a continuous fermentation with immobilised R. delemar were used to explore the effect of the three factors on regime 1 and 2. Insoluble solids were found to have no effect on the response in either regime 1 or 2. Increasing the aqueous calcium ion concentrations to 10 g L−1 resulted in a three-fold increase in MA titres (regime 1). Moreover, an increase in pH above 7 was associated with a drop in FA concentrations in regime 2. Further tests established that this was due to the hydration of FA to MA, influenced by high pH conditions (7 or higher), nitrogen starvation, and glucose depletion. Anaerobic conditions were also found to significantly improve the hydration process. This study presents the first investigation in which the production of FA followed by in situ hydration of FA to MA with R. delemar has been achieved. Full article
(This article belongs to the Special Issue Biotransformation Catalyzed by Immobilized Enzyme)
►▼ Show Figures

Figure 1

12 pages, 798 KiB  
Article
Batch and Flow Nitroaldol Synthesis Catalysed by Granulicella tundricola Hydroxynitrile Lyase Immobilised on Celite R-633
by José Coloma, Lidwien Teeuwisse, Muhammad Afendi, Peter-Leon Hagedoorn and Ulf Hanefeld
Catalysts 2022, 12(2), 161; https://doi.org/10.3390/catal12020161 - 27 Jan 2022
Cited by 6 | Viewed by 2606
Abstract
Granulicella tundricola hydroxynitrile lyase (GtHNL) catalyses the synthesis of chiral (R)-cyanohydrins and (R)-β-nitro alcohols. The triple variant GtHNL-A40H/V42T/Q110H (GtHNL-3V) was immobilised on Celite R-633 and used in monophasic MTBE saturated with 100 mM KPi [...] Read more.
Granulicella tundricola hydroxynitrile lyase (GtHNL) catalyses the synthesis of chiral (R)-cyanohydrins and (R)-β-nitro alcohols. The triple variant GtHNL-A40H/V42T/Q110H (GtHNL-3V) was immobilised on Celite R-633 and used in monophasic MTBE saturated with 100 mM KPi buffer pH 7 for the synthesis of (R)-2-nitro-1-phenylethanol (NPE) in batch and continuous flow systems. Nitromethane was used as a nucleophile. A total of 82% of (R)-NPE and excellent enantioselectivity (>99%) were achieved in the batch system after 24 h of reaction time. GtHNL-3V on Celite R-633 was successfully recycled five times. During more recycling steps a significant decrease in yield was observed while the enantioselectivity remained excellent over eight cycles. The use of a flow system enabled the continuous synthesis of (R)-NPE. A total of 15% formation of (R)-NPE was reached using a flow rate of 0.1 mL min−1; unfortunately, the enzyme was not stable, and the yield decreased to 4% after 4 h on stream. A similar yield was observed during 15 h at a rate of 0.01 mL min−1. Surprisingly the use of a continuous flow system did not facilitate the process intensification. In fact, the batch system displayed a space-time-yield (STY/mgenzyme) of 0.10 g L−1 h−1 mgenzyme−1 whereas the flow system displayed 0.02 and 0.003 g L−1 h−1 mgenzyme−1 at 0.1 and 0.01 mL min−1, respectively. In general, the addition of 1 M nitromethane potentially changed the polarity of the reaction mixture affecting the stability of Celite-GtHNL-3V. The nature of the batch system maintained the reaction conditions better than the flow system. The higher yield and productivity observed for the batch system show that it is a superior system for the synthesis of (R)-NPE compared with the flow approach. Full article
(This article belongs to the Special Issue Biotransformation Catalyzed by Immobilized Enzyme)
►▼ Show Figures

Figure 1

13 pages, 3219 KiB  
Article
Continuous Diastereomeric Kinetic Resolution—Silybins A and B
by David Biedermann, Martina Hurtová, Oldřich Benada, Kateřina Valentová, Lada Biedermannová and Vladimír Křen
Catalysts 2021, 11(9), 1106; https://doi.org/10.3390/catal11091106 - 14 Sep 2021
Cited by 5 | Viewed by 2688
Abstract
The natural diastereomeric mixture of silybins A and B is often used (and considered) as a single flavonolignan isolated from the fruit extract of milk thistle (Silybum marianum), silymarin. However, optically pure silybin diastereomers are required for the evaluation of their [...] Read more.
The natural diastereomeric mixture of silybins A and B is often used (and considered) as a single flavonolignan isolated from the fruit extract of milk thistle (Silybum marianum), silymarin. However, optically pure silybin diastereomers are required for the evaluation of their biological activity. The separation of silybin diastereomers by standard chromatographic methods is not trivial. Preparative chemoenzymatic resolution of silybin diastereomers has been published, but its optimization and scale-up are needed. Here we present a continuous flow reactor for the chemoenzymatic kinetic resolution of silybin diastereomers catalyzed by Candida antarctica lipase B (CALB) immobilized on acrylic resin beads (Novozym® 435). Temperature, flow rate, and starting material concentration were varied to determine optimal reaction conditions. The variables observed were conversion and diastereomeric ratio. Optimal conditions were chosen to allow kilogram-scale reactions and were determined to be −5 °C, 8 g/L silybin, and a flow rate of 16 mL/min. No significant carrier degradation was observed after approximately 30 cycles (30 days). Under optimal conditions and using a 1000 × 15 mm column, 20 g of silybin per day can be easily processed, yielding 6.7 and 5.6 g of silybin A and silybin B, respectively. Further scale-up depends only on the size of the reactor. Full article
(This article belongs to the Special Issue Biotransformation Catalyzed by Immobilized Enzyme)
►▼ Show Figures

Graphical abstract

Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:
 
Displaying articles 1-3
Back to TopTop