Adipose-Derived Stem Cells for Tissue Regeneration

A special issue of Cells (ISSN 2073-4409). This special issue belongs to the section "Tissues and Organs".

Deadline for manuscript submissions: 30 November 2025 | Viewed by 785

Special Issue Editor


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Guest Editor
1. University Center of Plastic, Aesthetic, Hand and Reconstructive Surgery, University of Regensburg, Regensburg, Germany
2. Applied Stem Cell Research Center, Regensburg, Germany
Interests: tissue engineering; stem cells; adipose stem cells, tissue perfusion; organ and tissue regeneration; organ and tissue transplantation
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Dear Colleagues,

Stem cells offer a promising avenue for the repair and regeneration of both acutely and chronically damaged tissues following injury, as well as for the restoration of tissue lost after tumor resection. Their potential applications are broad, ranging from the regeneration of tendons, nerves, and cartilage to skin rejuvenation.

Mesenchymal stem cells, and, in particular, adipose-derived stem cells, can be harvested in high quantities from various tissue sources. However, many aspects regarding their collection, preparation, and clinical use remain unresolved, and further research is needed to optimize their therapeutic potential.

For this Special Issue titled “Adipose-Derived Stem Cells for Tissue Regeneration”, we invite authors to submit original research articles or reviews focusing on specific and innovative approaches to tissue repair, especially those highlighting clinical applications.

Prof. Dr. Dr. Lukas Prantl, MD, PhD, MHBA, FACS
Guest Editor

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Keywords

  • adipose stem cells (ASCs)
  • stromal vascular fraction (SVF)
  • cell enriched lipotransfer (CELT and CELT-PLUS)
  • extracellular vesicles (EVs)
  • tissue processing
  • basic biology
  • mechanisms of action
  • regenerative medicine
  • cell-bases therapies

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Published Papers (1 paper)

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Research

15 pages, 2310 KB  
Article
CELTPLUS Fat Increases the Metabolic Activity as Well as the SVF-Yield Significantly When Compared to CELT Fat, Even After Cryopreservation with DMSO
by Tom Schimanski, Lukas Prantl, Andreas Eigenberger, Oliver Felthaus, Rafael Loucas, Kirsten Utpatel and Kerstin Steer
Cells 2025, 14(16), 1270; https://doi.org/10.3390/cells14161270 - 17 Aug 2025
Viewed by 530
Abstract
Lipofilling has far more applications than cosmetic surgery alone. Due to its high content of stromal vascular fraction (SVF) cells, lipoaspirate can also be used to treat wounds, as its cellular components may accelerate wound healing. Using our CELTPLUS protocol, we can [...] Read more.
Lipofilling has far more applications than cosmetic surgery alone. Due to its high content of stromal vascular fraction (SVF) cells, lipoaspirate can also be used to treat wounds, as its cellular components may accelerate wound healing. Using our CELTPLUS protocol, we can increase the number of SVF cells per volume. Unfortunately, some patients require more than one treatment to achieve an optimal outcome, but would unnecessarily suffer from repeated liposuction. Therefore, our objective was to test whether cryopreserving CELTPLUS fat could offer a solution, potentially avoiding the need for repeated liposuction procedures. DMSO was used as a cryoprotective agent for proof-of-principle testing, although other non-toxic cryoprotective agents should be considered in the future. The rest of our freezing protocol is a clinically friendly attempt to facilitate the translation into clinical practice. We tested the cryopreserved tissue using histological evaluation, metabolism measurement, SVF cell yield estimation, PCRs from both whole tissue and from cultured SVF cells, and Oil Red “O” staining. We found that freezing CELTPLUS fat with DMSO yields better results than without cryoprotection in all evaluated methods. Until non-toxic cryoprotective agents are tested on CELTPLUS fat, we do not recommend initiating animal or human testing. Full article
(This article belongs to the Special Issue Adipose-Derived Stem Cells for Tissue Regeneration)
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