Genomics and High-Throughput Phenotyping to Characterize Forest Genetic Resources for Conservation and Breeding

A special issue of Forests (ISSN 1999-4907). This special issue belongs to the section "Genetics and Molecular Biology".

Deadline for manuscript submissions: closed (31 December 2023) | Viewed by 1944

Special Issue Editors


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Guest Editor
Laboratory of Forest Genetics and Tree Improvement, School of Forestry and Natural Environment, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece
Interests: forests genetics; tree breeding and selection strategies; conservation and use of forest genetic resources; forest genetic monitoring; quantitative, evolutionary and ecological genetics; classical and molecular breeding; genetic adaptation to climate change, assisted migration; application of GIS in conservation of genetic resources
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Guest Editor
Faculty of Forest Sciences and Ecology, Vytautas Magnus University, LT-44248 Kaunas, Lithuania
Interests: forestry; forest genetics; population genetics; ecology; evolutionary genetics; conservation and use of forest genetic resources; interspecific hybridization; forest genetic monitoring; traceability of forest reproductive material; etc.

Special Issue Information

Dear Colleagues,

The advancements of technology in genomic and high-throughput phenotyping tools is creating new opportunities and challenges for the characterization of forest genetic resources (FGR), in terms of rarity, endangered status, marginality and genetic distinctiveness, plasticity, specific adaptations and tolerance to biotic and abiotic stresses. The information obtained by the use of these tools separately, but most preferably in an integrated manner, will constitute a basis for assessing the genetic and adaptive variation levels of FGR, the representativeness of their conservation efforts so far, their in situ and ex situ conservation needs, their resilience to biotic and abiotic factors, as well as their potential for use in forest tree breeding programs.

The aim of the Special Issue is to compile and present cutting-edge studies which focus on the use of genetic/genomic and high-throughput phenotyping methods in order to monitor and characterize FGR in terms of distinctiveness and genetic/adaptive variation. The development of such techniques will allow the detection of conservation gaps, as well as the identification of genetic resources that could be of significance for conservation and/or breeding programs. 

Dr. Evi Alizoti
Dr. Darius Kavaliauskas
Guest Editors

Manuscript Submission Information

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Keywords

  • genomics
  • phenotyping
  • forest genetic resources (FGR)
  • FGR conservation
  • forest tree breeding

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Published Papers (1 paper)

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Research

23 pages, 20547 KiB  
Article
Single-Cell Transcriptome Atlas of Leaves at Different Developmental Stages in Populus alba × Populus glandulosa Clone 84K
by Yanchun Jing, Yongyu Ren, Shuwen Zhang and Xiangyang Kang
Forests 2024, 15(3), 512; https://doi.org/10.3390/f15030512 - 9 Mar 2024
Viewed by 1542
Abstract
Leaves are crucial photosynthetic plant organs. The development of poplar leaves has spatio-temporal specificity and it is of great significance to study the single-cell transcription atlas of leaves to reveal the temporal regulation of gene expression in different cell types. Here, single-cell RNA [...] Read more.
Leaves are crucial photosynthetic plant organs. The development of poplar leaves has spatio-temporal specificity and it is of great significance to study the single-cell transcription atlas of leaves to reveal the temporal regulation of gene expression in different cell types. Here, single-cell RNA sequencing was performed on 17,768 tender leaf and 5846 functional leaf cells of Poplar 84K to construct a transcriptome atlas and developmental trajectory. The results showed that there were five and six cell types in tender and functional leaves, respectively. According to a pseudo-time trajectory analysis and the clustering of expressed genes into different cell types, the development of tender and functional leaves was divided into two temporal stages. Tender leaf epidermal cells developed earliest and were enriched with genes related to cell division and growth, indicating that tender leaves were in the stage of cell expansion and functional differentiation. Functional leaf palisade mesophyll cells were enriched with genes related to photosynthesis and carbon metabolism and cell types performing different functions tended to mature, indicating that functional leaves were in the stage of leaf development and the initial formation of photosynthesis. Our in-depth analysis of the transcriptional regulation at the single-cell level during leaf development provides an important basis for studying the mechanisms involved in cell differentiation and leaf development in poplar as well as other plants. Full article
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