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Second Edition: Clinical Microbiology and Infectious Diseases

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Department of Molecular and Translational Medicine, Institute of Microbiology, Università degli Studi di Brescia, 25123 Brescia, Italy
Interests: antibiotic resistance; antiviral resistance; molecular epidemiology
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

The discovery of new pathogens, emerging infections, the acquisition of new resistance mechanisms via microorganisms and the introduction of more and more sophisticated laboratory techniques means that competence is imperative in regard to clinical microbiology and infectious diseases.

Clinical microbiology laboratories have a leading role in the success of antimicrobial stewardship programs because they provide information that enables an accurate diagnosis and aids in the therapy of patients.

Over the past decade, rapid diagnostic assays have been developed, such as matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS), the peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) technique, multiplex nucleic acid assays and the quantitative polymerase chain reaction (qPCR). All of these methods can provide information regarding the type of pathogen involved in the infective process and the presence of resistance genes in a shorter timeframe compared with traditional assays.

Any submissions which cover the following topics are encouraged:  molecular mechanisms of pathogenicity, microorganism genomics able to elucidate their virulence factors, antibiotic resistance, new diagnostic assays, nosocomial infections and all basic and applied research relevant in the field of clinical microbiology and infectious diseases.

Dr. Maria Antonia De Francesco
Guest Editor

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Keywords

  • bacteria
  • virus
  • diagnostic assays
  • innate immunity
  • adaptive immunity
  • vaccines
  • antibiotic resistance
  • WGS

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Related Special Issue

Published Papers (1 paper)

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Research

19 pages, 3958 KiB  
Article
Effectiveness Evaluation of a UV-C-Photoinactivator against Selected ESKAPE-E Pathogens
by Karyne Rangel, Fellipe O. Cabral, Guilherme C. Lechuga, Maria H. S. Villas-Bôas, Victor Midlej and Salvatore G. De-Simone
Int. J. Environ. Res. Public Health 2022, 19(24), 16559; https://doi.org/10.3390/ijerph192416559 - 9 Dec 2022
Cited by 5 | Viewed by 1801
Abstract
Healthcare-associated infections (HAI) worldwide includes infections by ESKAPE-E pathogens. Environmental surfaces and fomites are important components in HAI transmission dynamics, and shoe soles are vectors of HAI. Ultraviolet (UV) disinfection is an effective method to inactivate pathogenic microorganisms. In this study, we investigated [...] Read more.
Healthcare-associated infections (HAI) worldwide includes infections by ESKAPE-E pathogens. Environmental surfaces and fomites are important components in HAI transmission dynamics, and shoe soles are vectors of HAI. Ultraviolet (UV) disinfection is an effective method to inactivate pathogenic microorganisms. In this study, we investigated whether the SANITECH UV-C shoe sole decontaminator equipment that provides germicidal UV-C radiation could effectively reduce this risk of different pathogens. Six standard strains and four clinical MDR strains in liquid and solid medium were exposed to a UV-C System at specific concentrations at other times. Bacterial inactivation (growth and cultivability) was investigated using colony counts and resazurin as metabolic indicators. SEM was performed to assess the membrane damage. Statistically significant reduction in cell viability for all ATCCs strains occurred after 10 s of exposure to the UV-C system, except for S. enterica, which only occurred at 20 s. The cell viability of P. aeruginosa (90.9%), E. faecalis and A. baumannii (85.3%), S. enterica (82.9%), E. coli (79.2%) and S. aureus (71.9%) was reduced considerably at 20 s. In colony count, after 12 s of UV-C exposure, all ATCC strains showed a 100% reduction in CFU counts, except for A. baumannii, which reduced by 97.7%. A substantial reduction of colonies above 3 log10 was observed at 12 and 20 s in all bacterial strains tested, except for A. baumannii ATCC 19606 (12 s). The exposure of ATCCs bacterial strains to the UV-C system for only 2 s was able to reduce 100% in the colony forming units (CFU) count in all ATCCs strains, S. aureus, P. aeruginosa, E. coli, A. baumannii, E. faecalis, except the S. enterica strain which had a statistically significant reduction of 99.7%. In ATCC strains, there was a substantial decrease in colonies after 4 s (sec) of exposure to the UV-C system, with a reduction ranging from 3.78–4.15 log10 CFU/mL. This reduction was observed in MDR/ESKAPE-E strains within 10 s, showing that UV-C could eliminate above 3.84 log10 CFU/mL. SEM showed a reduction of pili-like appendages after UV-C treatment in all strains except for E. coli (ATCC 25922). The Sanitech UV-C shoe sole decontaminator equipment from Astech Serv. and Fabrication Ltd. (Petrópolis, Brazil), effectively killed in vitro a series of ATCCs and MDR/ESKAPE-E bacteria of sanitary interest, commonly found in the hospital environment. Full article
(This article belongs to the Special Issue Second Edition: Clinical Microbiology and Infectious Diseases)
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