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Molecular Studies of Hematological Malignancies: Emphasis on Development of Flow-Cytometry and Cell-Sorting-Based Technics for Improving Diagnosis

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Pathology, Diagnostics, and Therapeutics".

Deadline for manuscript submissions: closed (31 October 2024) | Viewed by 2029

Special Issue Editors


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Guest Editor
1. Hematology Unit and Laboratories, Galilee Medical Center, Nahariya 22100, Israel
2. Azrieli Faculty of Medicine, Bar-Ilan University, Safed 1311502, Israel
Interests: hematological malignancies; laboratory aspects of hematological malignancies, soluable and flow-cytometry based biomarkers for diagnosis, classification, prognosis, tailored treatment, treatment respnse and treatment side effects

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Guest Editor
The Ruth and Bruce Rappaport Faculty of Medicine, Department of Hematology and BMT, Rambam Health Care Campus, Haifa, Technion, Israel institute of Technology, Haifa, Israel
Interests: hematology

Special Issue Information

Dear Colleagues,

The diagnosis and classification of hematological malignancies and other blood-related disorders are critical for disease treatment. Improving the diagnostic work of the hematology laboratory is also the main target of many researchers. The development of flow cytometry and cell sorting-based techniques could play an important role in the improvement of diagnosis of blood cancers in general, and in minimally invasive tissue biopsies in particular. Understanding the diagnostic potential of soluble blood levels of the Brain-Derived Neurotrophic Factor (BDNF) in hematological diseases may also bring great inspiration for scientists.

In this Special Issue, we would like to call upon researchers, laboratory scientists, clinicians, and co-workers in the field of Hematology to share their knowledge and experience in new developments and applications using molecular techniques, such as cell-sorting techniques and flow cytometry, in hematological malignancies and other hematological diseases. In addition, submissions on the use of soluble protein-based blood biomarkers, which have the potential to improve diagnosis, prognosis, response to treatment, and therapy-related side effects in hematological diseases are also welcomed. Of special interest are studies demonstrating immediate potential for implementation in the daily laboratory workup.

Dr. Azoulay David
Dr. Netanel A Horowitz
Guest Editors

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Keywords

  • laboratory hematology
  • hematological malignancies
  • flow cytometry and cell sorting
  • soluble biomarkers

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Published Papers (1 paper)

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Research

12 pages, 1959 KiB  
Article
The Involvement of LAG-3positive Plasma Cells in the Development of Multiple Myeloma
by Natalia Kreiniz, Nasren Eiza, Tamar Tadmor, Ilana Levy Yurkovski, Sarah Matarasso Greenfeld, Adi Sabag, Raeda Mubariki, Celia Suriu, Ekaterina Votinov, Elias Toubi and Zahava Vadasz
Int. J. Mol. Sci. 2024, 25(1), 549; https://doi.org/10.3390/ijms25010549 - 31 Dec 2023
Cited by 1 | Viewed by 1369
Abstract
The Lymphocyte-Activation Protein 3 (LAG-3) inhibitory receptor is expressed on regulatory plasma cells (PCs). Micro-environmental cells that express LAG-3 were found to be increased during the progression of smoldering multiple myeloma (SMM). To assess the possible role of LAG-3 expression on regulatory PCs [...] Read more.
The Lymphocyte-Activation Protein 3 (LAG-3) inhibitory receptor is expressed on regulatory plasma cells (PCs). Micro-environmental cells that express LAG-3 were found to be increased during the progression of smoldering multiple myeloma (SMM). To assess the possible role of LAG-3 expression on regulatory PCs in patients with plasma cell dyscrasia. Purified Cluster of Differentiation 138 (CD138+) PCs from patients with premalignant conditions, active multiple myeloma (MM), and controls were analyzed for the expression of LAG-3 by flow cytometry. Autologous CD8+T cells were incubated with sorted LAG-3pos or LAG-3neg PCs for 24 h. The expression of granzyme (Grz) in CD8+T cells was assessed by flow cytometry. LAG-3 expression on PCs in active MM (newly diagnosed and relapse refractory MM) was significantly increased compared to monoclonal gammopathy of undetermined significance (MGUS)/ SMM. Grz expression was significantly decreased in CD8+T cells incubated with CD138+LAG-3pos PCs, compared to CD138+LAG-3neg PCs in patients with plasma cell dyscrasia, n = 31, p = 0.0041. LAG-3 expression on malignant PCs can be involved in the development of MM from MGUS by decreasing the expression of Grz in CD8+T cells. Full article
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