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Responses to Abiotic and Biotic Stresses of Gene-Edited Crop Plants

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Plant Sciences".

Deadline for manuscript submissions: 31 July 2024 | Viewed by 575

Special Issue Editor


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Guest Editor
Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Rishon LeZion 7505101, Israel
Interests: plant molecular biology; genetic engineering; genome editing; abiotic stress tolerance in plants
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Special Issue Information

Dear Colleagues,

Various abiotic and biotic stresses can affect plant growth and development at physiological, biochemical, and molecular levels, including alterations in gene expression, accumulation of organic solutes, imbalance in phytohormones excretion, and inhibition of plant growth. Abiotic stresses, viz. temperature, drought, salinity, heavy metals and floods and biotic stresses such as insects, nematodes, fungi and bacteria cause severe damage to important crop plants worldwide. A better understanding of plants' structural and functional genomics helps to fabricate new plant varieties more efficiently and rapidly to sustain their performance under different stress and climate change scenarios. Gene discovery and functional genomics have disclosed diverse mechanisms and gene families, which confer enhanced productivity and adaptation to environmental stresses. Next-generation genomics and functional validation approaches have provided further opportunities to delve deep into the molecular mechanisms and guide the genetic improvement strategies to breed next-generation plant species. Various gene editing tools, including popularly used CRISPR/CAS9 techniques, are potentially powerful gene editing methods used to mitigate the effect of different environmental stresses. In this Special Issue of the International Journal of Molecular Sciences, we aim to publish high-quality research articles and reviews on understanding plant gene expression and the mitigation strategies of various environmental stresses using more advanced gene editing tools.

Dr. Avinash Chandra Rai is a scientist (Topical Advisory Panel in IJMS), who will assist Dr. Manoj Kumar in managing this Special Issue.

Dr. Manoj Kumar
Guest Editor

Manuscript Submission Information

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Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

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Keywords

  • abiotic stresses
  • biotic stresses
  • CRISPR-Cas9
  • genetic engineering
  • gene editing
  • crop productivity

Published Papers (1 paper)

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Research

17 pages, 4758 KiB  
Article
OsPUB9 Gene Edited by CRISPR/Cas9 Enhanced Resistance to Bacterial Leaf Blight in Rice (Oryza sativa L.)
by Me-Sun Kim, Van Trang Le, Yu Jin Jung, Kwon-Kyoo Kang and Yong-Gu Cho
Int. J. Mol. Sci. 2024, 25(13), 7145; https://doi.org/10.3390/ijms25137145 - 28 Jun 2024
Viewed by 285
Abstract
Ubiquitination plays a crucial role in regulating signal pathways during the post-translation stage of protein synthesis in response to various environmental stresses. E3 ubiquitin ligase has been discovered to ultimately control various intracellular activities by imparting specificity to proteins to be degraded. This [...] Read more.
Ubiquitination plays a crucial role in regulating signal pathways during the post-translation stage of protein synthesis in response to various environmental stresses. E3 ubiquitin ligase has been discovered to ultimately control various intracellular activities by imparting specificity to proteins to be degraded. This study was conducted to confirm biological and genetic functions of the U-box type E3 ubiquitin ligase (PUB) gene against biotic stress in rice (Oryza sativa L.). OsPUB9 gene-specific sgRNA were designed and transformants were developed through Agrobacterium-mediated transformation. Deep sequencing using callus was performed to confirm the mutation type of T0 plants, and a total of three steps were performed to select null individuals without T-DNA insertion. In the case of the OsPUB9 gene-edited line, a one bp insertion was generated by gene editing, and it was confirmed that early stop codon and multiple open reading frame (ORF) sites were created by inserting thymine. It is presumed that ubiquitination function also changed according to the change in protein structure of U-box E3 ubiquitin ligase. The OsPUB9 gene-edited null lines were inoculated with bacterial leaf blight, and finally confirmed to have a resistance phenotype similar to Jinbaek, a bacterial blight-resistant cultivar. Therefore, it is assumed that the amino acid sequence derived from the OsPUB9 gene is greatly changed, resulting in a loss of the original protein functions related to biological mechanisms. Comprehensively, it was confirmed that resistance to bacterial leaf blight stress was enhanced when a mutation occurred at a specific site of the OsPUB9 gene. Full article
(This article belongs to the Special Issue Responses to Abiotic and Biotic Stresses of Gene-Edited Crop Plants)
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