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High-Throughput Molecular Function Screens
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High-Throughput Molecular Function Screens

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Biology".

Deadline for manuscript submissions: closed (31 January 2021) | Viewed by 8003

Special Issue Editors

Prof. Dr. Martin R. Schiller

E-Mail Website
Guest Editor
1. Nevada Institute of Personalized Medicine, University of Nevada Las Vegas, Las Vegas, NV 89154-4004, USA
2. School of Life Sciences, University of Nevada Las Vegas, Las Vegas, NV 89154-4004, USA
Interests: bioinformatics; virology; personalized medicine; functional genomics; minimotifs; HIV; NHEJ; gene editing; high-throughput screening
Special Issues, Collections and Topics in MDPI journals
Dr. Ronald Benjamin Babu

E-Mail Website
Guest Editor
Nevada Institute of Personalized Medicine and School of Life Sciences, University of Nevada Las Vegas, Las Vegas, NV 89154-4004, USA
Interests: virology; functional genomics; HIV; NHEJ; gene editing; high-throughput screening; Infection biology
Prof. Nora B. Caberoy

E-Mail Website
Guest Editor
School of Life Sciences, University of Nevada Las Vegas, Las Vegas, NV 89154-4004, USA
Interests: functional proteomics; phagocytosis; retinal cell biology; retinal degenerative diseases; alzheimer’s disease; obesity; molecular and cell biology; phage display

Special Issue Information

Dear Colleagues,

High-throughput screening (HTS) technologies have transformed the rate of research within the biomedical sciences. While some of these technologies identify nucleic acid sequences, gene expression profiles, metabolites, or candidate drugs, many are now focused on different molecular functions. Technologies that define molecular functional relationships have the potential to systematize our understanding of the mechanistic basis driving cell process and their vulnerabilities that manifest as disease. In this Special Issue, the collection of review articles covers both the state-of-the-art functional high throughput screening technologies, and the future prospects for impacting biomedical sciences.

This volume will represent a comprehensive collection of information related to this growing field. I hope that you are willing to prepare a comprehensive review related to an area related in High-throughput Molecular Function Screens that has not yet been covered in this Special Issue. The topics we intend to cover do not merely identify a class or the nature of molecules like metabolomics or transcriptomics, but rather identify functional connection between biomolecules. Topics we intend to cover are functional proteomics (phage display, yeast 2- hybrid, peptide arrays, affinity MS/MS), functional genomics (RNAi, shRNA, and CRISPR/Cas screening, CHIP-Seq, massively parallel functional genomic screens, functional NGS, microarrays or RNA-Seq as applied to assaying transcription factors), high throughput affinity capture/enzymatic binding or activity based screens, as well as the algorithms and analytical tools required to mine high throughput screening data.

It would be greatly appreciated if you would contact Ronald Babu, one of the guest editors, indicating whether you agree to cover one of these or a related topic by proposing a title and abstract. If you cannot write a review for this issue, please feel free to suggest someone with the appropriate expertise.

We are looking forward to your favorable reply.

Prof. Dr. Martin R. Schiller
Dr. Ronald Benjamin Babu
Prof. Nora B. Caberoy
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. There is an Article Processing Charge (APC) for publication in this open access journal. For details about the APC please see here. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • High throughput functional screens
  • Functional genomics
  • Functional screening
  • Assay development
  • High Content Screening
  • Algorithms and Analytical methods

Published Papers (2 papers)

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Research

17 pages, 3475 KiB  
Article
A De novo Peptide from a High Throughput Peptide Library Blocks Myosin A -MTIP Complex Formation in Plasmodium falciparum
by Zill e Anam, Nishant Joshi, Sakshi Gupta, Preeti Yadav, Ayushi Chaurasiya, Amandeep Kaur Kahlon, Shikha Kaushik, Manoj Munde, Anand Ranganathan and Shailja Singh
Int. J. Mol. Sci. 2020, 21(17), 6158; https://doi.org/10.3390/ijms21176158 - 26 Aug 2020
Cited by 7 | Viewed by 3409
Abstract
Apicomplexan parasites, through their motor machinery, produce the required propulsive force critical for host cell-entry. The conserved components of this so-called glideosome machinery are myosin A and myosin A Tail Interacting Protein (MTIP). MTIP tethers myosin A to the inner membrane complex of [...] Read more.
Apicomplexan parasites, through their motor machinery, produce the required propulsive force critical for host cell-entry. The conserved components of this so-called glideosome machinery are myosin A and myosin A Tail Interacting Protein (MTIP). MTIP tethers myosin A to the inner membrane complex of the parasite through 20 amino acid-long C-terminal end of myosin A that makes direct contacts with MTIP, allowing the invasion of Plasmodium falciparum in erythrocytes. Here, we discovered through screening a peptide library, a de-novo peptide ZA1 that binds the myosin A tail domain. We demonstrated that ZA1 bound strongly to myosin A tail and was able to disrupt the native myosin A tail MTIP complex both in vitro and in vivo. We then showed that a shortened peptide derived from ZA1, named ZA1S, was able to bind myosin A and block parasite invasion. Overall, our study identified a novel anti-malarial peptide that could be used in combination with other antimalarials for blocking the invasion of Plasmodium falciparum. Full article
(This article belongs to the Special Issue High-Throughput Molecular Function Screens)
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13 pages, 2336 KiB  
Article
An efficient Screening System in Yeast to Select a Hyperactive piggyBac Transposase for Mammalian Applications
by Wen Wen, Shanshan Song, Yuchun Han, Haibin Chen, Xiangzhen Liu and Qijun Qian
Int. J. Mol. Sci. 2020, 21(9), 3064; https://doi.org/10.3390/ijms21093064 - 26 Apr 2020
Cited by 9 | Viewed by 4245
Abstract
As non-viral transgenic vectors, the piggyBac transposon system represents an attractive tool for gene delivery to achieve a long-term gene expression in immunotherapy applications due to its large cargo capacity, its lack of a trace of transposon and of genotoxic potential, and its [...] Read more.
As non-viral transgenic vectors, the piggyBac transposon system represents an attractive tool for gene delivery to achieve a long-term gene expression in immunotherapy applications due to its large cargo capacity, its lack of a trace of transposon and of genotoxic potential, and its highly engineered structure. However, further improvements in transpose activity are required for industrialization and clinical applications. Herein, we established a one-plasmid effective screening system and a two-step high-throughput screening process in yeast to isolate hyperactive mutants for mammalian cell applications. By applying this screening system, 15 hyperactive piggyBac transposases that exhibited higher transpose activity compared with optimized hyPBase in yeast and four mutants that showed higher transpose activity in mammalian cells were selected among 3000 hyPBase mutants. The most hyperactive transposase, bz-hyPBase, with four mutation sites showed an ability to yield high-efficiency editing in Chinese hamster ovarian carcinoma (CHO) cells and T cells, indicating that they could be expanded for gene therapy approaches. Finally, we tested the potential of this screening system in other versions of piggyBac transposase. Full article
(This article belongs to the Special Issue High-Throughput Molecular Function Screens)
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