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Molecular Toxicity of Foodstuff Contaminants and Mitigation Strategies

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Toxicology".

Deadline for manuscript submissions: 20 December 2024 | Viewed by 921

Special Issue Editors


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Guest Editor
State Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China
Interests: mycotoxin; biodegradation of mycotoxin; molecular toxicity of foodstuff contaminants; nutritional, physical, chemical and biological mitigation strategies
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Guest Editor
Prestage Department of Poultry Science, North Carolina State University, Raleigh, NC 27695, USA
Interests: immunology; virology; host-pathogen interactions; biological mitigation strategies

Special Issue Information

Dear Colleagues,

Foodstuff safety is related to human and animal health. Contaminants including antibiotics, heavy metals, pesticides, mycotoxins, pathogens, microplastics, and polycyclic aromatic hydrocarbons are the most important risk factors affecting food and feed safety. They can be naturally contaminated with grain seed or introduced into food and feed products during production, handling, or storage. These contaminants cause immunotoxicity, reproductive toxicity, hepatotoxicity, cytotoxicity, and seriously threaten human and animal health.

Supervised by Dr. Lihong Zhao and Dr. Matthew Koci, and assisted by our Topical Advisory Panel Member Dr. Yongpeng Guo from Henan Agricultural University and Ms. Xin Fang from China Agricultural University, the Special Issue “Molecular Toxicity of Foodstuff Contaminants and Mitigation Strategies”, aims to collect papers about the latest advances in the molecular toxicity mechanisms of urgent food contaminants and bring about promising mitigation strategies to reduce their threat to human and animal health.

Topics of interest include but are not limited to the following:

  • The use of multi-omics methods to provide deep insights into the molecular toxicity mechanisms and the combined toxic effects of common contaminants in food and feed.
  • Novel nutritional strategies for regulating the resistance and metabolism ability of humans and animals to contaminants in food and feed.
  • Promising physical, chemical, and microbial methods to detoxify contaminants in food and feed, with a specific focus on the characterization and application of microbial enzymes in food and feed.

Dr. Lihong Zhao
Dr. Matthew Koci
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. There is an Article Processing Charge (APC) for publication in this open access journal. For details about the APC please see here. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • antibiotics
  • heavy metals
  • pesticides
  • mycotoxins
  • foodborne pathogens
  • polycyclic aromatic hydrocarbons
  • microplastics
  • toxicity
  • biodegradation
  • detoxification

Published Papers (1 paper)

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Research

11 pages, 3610 KiB  
Article
Combined Strategies for Improving Aflatoxin B1 Degradation Ability and Yield of a Bacillus licheniformis CotA-Laccase
by Yanrong Liu, Limeng Liu, Zhenqian Huang, Yongpeng Guo, Yu Tang, Yanan Wang, Qiugang Ma and Lihong Zhao
Int. J. Mol. Sci. 2024, 25(12), 6455; https://doi.org/10.3390/ijms25126455 - 12 Jun 2024
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Abstract
Aflatoxin B1 (AFB1) contamination is a serious threat to nutritional safety and public health. The CotA-laccase from Bacillus licheniformis ANSB821 previously reported by our laboratory showed great potential to degrade AFB1 without redox mediators. However, the use of this [...] Read more.
Aflatoxin B1 (AFB1) contamination is a serious threat to nutritional safety and public health. The CotA-laccase from Bacillus licheniformis ANSB821 previously reported by our laboratory showed great potential to degrade AFB1 without redox mediators. However, the use of this CotA-laccase to remove AFB1 in animal feed is limited because of its low catalytic efficiency and low expression level. In order to make better use of this excellent enzyme to effectively degrade AFB1, twelve mutants of CotA-laccase were constructed by site-directed mutagenesis. Among these mutants, E186A and E186R showed the best degradation ability of AFB1, with degradation ratios of 82.2% and 91.8% within 12 h, which were 1.6- and 1.8-times higher than those of the wild-type CotA-laccase, respectively. The catalytic efficiencies (kcat/Km) of E186A and E186R were found to be 1.8- and 3.2-times higher, respectively, than those of the wild-type CotA-laccase. Then the expression vectors pPICZαA-N-E186A and pPICZαA-N-E186R with an optimized signal peptide were constructed and transformed into Pichia pastoris GS115. The optimized signal peptide improved the secretory expressions of E186A and E186R in P. pastoris GS115. Collectively, the current study provided ideal candidate CotA-laccase mutants for AFB1 detoxification in food and animal feed and a feasible protocol, which was desperately needed for the industrial production of CotA-laccases. Full article
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