Submit to IJMS Review for IJMS Propose a Special Issue

Journal Menu

Journal Browser

Molecular Recognition of Carbohydrates

Special Issue Editors
Special Issue Information
Keywords
Benefits of Publishing in a Special Issue
Published Papers

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Biochemistry".

Deadline for manuscript submissions: closed (31 December 2017) | Viewed by 61420

Share This Special Issue

Special Issue Editors

Prof. Francisco Javier Cañada

E-Mail Website1 Website2
Guest Editor

Chemical and Physical Biology, Centro de Investigaciones Biológicas, CIB-CSIC, Ramiro de Maeztu 9, 28040 Madrid, Spain
Interests: carbohydrates; molecular recognition; NMR

Prof. Dr. Jesus Jimenez-Barbero

E-Mail Website
Guest Editor

1. CIC bioGUNE, Bizkaia Technology Park, Building 800, 48160 Derio, Spain
2. Ikerbasque, Basque Foundation for Science, Maria López de Haro 3, 48013 Bilbao, Spain
Interests: NMR; molecular recognition; glycans; protein-ligand interactions; chemical biology; medicinal chemistry; infectious diseases; cancer; rare diseases; metabolomics
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Carbohydrates are among the most ubiquitous and complex types of biomolecules in nature. Although they are secondary gene products, and look like very simple molecules in terms of chemical functional groups (polyhydroxylated molecules with a formal carbonyl group), they support an astonishing capacity of carrying biological information, conforming to the so-called “glycan code”. The possibilities of diversity for their oligomeric structures (stereochemistry, branching, and secondary modifications) overwhelm those exerted by the other two key biomolecules, nucleic acids and proteins.

Carbohydrates play a very important role in intra- and intercellular molecular recognition events that trigger a variety of physiological and pathological events (glycoprotein fate and stability, fertilization, immunological responses, inflammation, cell defense or infection, cancer, etc).

Therefore, knowledge of their precise chemical structure with the maximum resolution possible, and access to key information on their interaction processes with different entities, such as lectins, enzymes, and antibodies, are essential for the understanding of many vital processes, as well as for opening the possibility of their modulation. To this aim, multidisciplinary research strategies (design and synthesis of natural glycans and their glycomimetics, development of glycoarrays and glyconanomaterials, physico-chemical characterization, NMR and X-ray structural determination, molecular modeling, biotechnological production, and cell biology protocols) are in continuous expansion in the field of glycosciences.

Prof. Francisco Javier Cañada
Prof. Jesus Jimenez-Barbero
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. There is an Article Processing Charge (APC) for publication in this open access journal. For details about the APC please see here. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

Glycosciences
Molecular recognition
Carbohydrate-protein interactions
Glycomimetics
Glycoarrays
Glycomics
Carbohydrate structure
Carbohydrate synthesis
Glyconanostructures (glyconanoparticles, glycan dendrimers)
NMR

Benefits of Publishing in a Special Issue

Ease of navigation: Grouping papers by topic helps scholars navigate broad scope journals more efficiently.
Greater discoverability: Special Issues support the reach and impact of scientific research. Articles in Special Issues are more discoverable and cited more frequently.
Expansion of research network: Special Issues facilitate connections among authors, fostering scientific collaborations.
External promotion: Articles in Special Issues are often promoted through the journal's social media, increasing their visibility.
e-Book format: Special Issues with more than 10 articles can be published as dedicated e-books, ensuring wide and rapid dissemination.

Further information on MDPI's Special Issue polices can be found here.

Published Papers (9 papers)

Download All Papers

Order results

Result details

Show export options Show export options

Select all

Export citation of selected articles as:

Research

Jump to: Review

13 pages, 1538 KiB

Open AccessArticle

A Combined NMR-Computational Study of the Interaction between Influenza Virus Hemagglutinin and Sialic Derivatives from Human and Avian Receptors on the Surface of Transfected Cells

by Francesca Vasile, Maddalena Panigada, Antonio Siccardi, Donatella Potenza and Guido Tiana

Int. J. Mol. Sci. 2018, 19(5), 1267; https://doi.org/10.3390/ijms19051267 - 24 Apr 2018

Cited by 14 | Viewed by 5031

Abstract

The development of small-molecule inhibitors of influenza virus Hemagglutinin could be relevant to the opposition of the diffusion of new pandemic viruses. In this work, we made use of Nuclear Magnetic Resonance (NMR) spectroscopy to study the interaction between two derivatives of sialic acid, Neu5Ac-α-(2,6)-Gal-β-(1–4)-GlcNAc and Neu5Ac-α-(2,3)-Gal-β-(1–4)-GlcNAc, and hemagglutinin directly expressed on the surface of recombinant human cells. We analyzed the interaction of these trisaccharides with 293T cells transfected with the H5 and H1 variants of hemagglutinin, which thus retain their native trimeric conformation in such a realistic environment. By exploiting the magnetization transfer between the protein and the ligand, we obtained evidence of the binding event, and identified the epitope. We analyzed the conformational features of the glycans with an approach combining NMR spectroscopy and data-driven molecular dynamics simulations, thus obtaining useful information for an efficient drug design. Full article

(This article belongs to the Special Issue Molecular Recognition of Carbohydrates)

► Show Figures

Graphical abstract

20 pages, 10767 KiB

Open AccessArticle

Lectin-Binding Specificity of the Fertilization-Relevant Protein PDC-109 by Means of Surface Plasmon Resonance and Carbohydrate REcognition Domain EXcision-Mass Spectrometry

by Sira Defaus, Manuel Avilés, David Andreu and Ricardo Gutiérrez-Gallego

Int. J. Mol. Sci. 2018, 19(4), 1076; https://doi.org/10.3390/ijms19041076 - 4 Apr 2018

Cited by 6 | Viewed by 4025

Abstract

Seminal plasma proteins are relevant for sperm functionality and some appear responsible for establishing sperm interactions with the various environments along the female genital tract towards the oocyte. In recent years, research has focused on characterizing the role of these proteins in the context of reproductive biology, fertility diagnostics and treatment of related problems. Herein, we focus on the main protein of bovine seminal plasma, PDC-109 (BSP-A1/-A2), which by virtue of its lectin properties is involved in fertilization. By means of surface plasmon resonance, the interaction of PDC-109 with a panel of the most relevant glycosidic epitopes of mammals has been qualitatively and quantitatively characterized, and a higher affinity for carbohydrates containing fucose has been observed, in line with previous studies. Additionally, using the orthogonal technique of Carbohydrate REcognition Domain EXcision-Mass Spectrometry (CREDEX-MS), the recognition domain of the interaction complexes between PDC-109 and all fucosylated disaccharides [(Fuc-α1,(3,4,6)-GlcNAc)] has been defined, revealing the specific glycotope and the peptide domain likely to act as the PDC-109 carbohydrate binding site. Full article

(This article belongs to the Special Issue Molecular Recognition of Carbohydrates)

► Show Figures

Figure 1

4129 KiB

Open AccessArticle

Glycosaminoglycan-Mediated Downstream Signaling of CXCL8 Binding to Endothelial Cells

by Rupert Derler, Bernd Gesslbauer, Corinna Weber, Elisabeth Strutzmann, Ingrid Miller and Andreas Kungl

Int. J. Mol. Sci. 2017, 18(12), 2605; https://doi.org/10.3390/ijms18122605 - 4 Dec 2017

Cited by 21 | Viewed by 4847

Abstract

The recruitment of leukocytes, mediated by endothelium bound chemokine gradients, is a vital process in inflammation. The highly negatively charged, unbranched polysaccharide family of glycosaminoglycans (GAGs), such as heparan sulfate and chondroitin sulfate mediate chemokine immobilization. Specifically the binding of CXCL8 (interleukin 8) to GAGs on endothelial cell surfaces is known to regulate neutrophil recruitment. Currently, it is not clear if binding of CXCL8 to GAGs leads to endothelial downstream signaling in addition to the typical CXCR1/CXCR2 (C-X-C motif chemokine receptor 1 and 2)-mediated signaling which activates neutrophils. Here we have investigated the changes in protein expression of human microvascular endothelial cells induced by CXCL8. Tumor necrosis factor alpha (TNFα) stimulation was used to mimic an inflammatory state which allowed us to identify syndecan-4 (SDC4) as the potential proteoglycan co-receptor of CXCL8 by gene array, real-time PCR and flow cytometry experiments. Enzymatic GAG depolymerization via heparinase III and chondroitinase ABC was used to emulate the effect of glycocalyx remodeling on CXCL8-induced endothelial downstream signaling. Proteomic analyses showed changes in the expression pattern of a number of endothelial proteins such as Zyxin and Caldesmon involved in cytoskeletal organization, cell adhesion and cell mobility. These results demonstrate for the first time a potential role of GAG-mediated endothelial downstream signaling in addition to the well-known CXCL8-CXCR1/CXCR2 signaling pathways in neutrophils. Full article

(This article belongs to the Special Issue Molecular Recognition of Carbohydrates)

► Show Figures

Figure 1

5355 KiB

Open AccessArticle

De Novo Assembly and Analysis of Polygonatum sibiricum Transcriptome and Identification of Genes Involved in Polysaccharide Biosynthesis

by Shiqiang Wang, Bin Wang, Wenping Hua, Junfeng Niu, Kaikai Dang, Yi Qiang and Zhezhi Wang

Int. J. Mol. Sci. 2017, 18(9), 1950; https://doi.org/10.3390/ijms18091950 - 12 Sep 2017

Cited by 52 | Viewed by 5644

Abstract

Polygonatum sibiricum polysaccharides (PSPs) are used to improve immunity, alleviate dryness, promote the secretion of fluids, and quench thirst. However, the PSP biosynthetic pathway is largely unknown. Understanding the genetic background will help delineate that pathway at the molecular level so that researchers can develop better conservation strategies. After comparing the PSP contents among several different P. sibiricum germplasms, we selected two groups with the largest contrasts in contents and subjected them to HiSeq2500 transcriptome sequencing to identify the candidate genes involved in PSP biosynthesis. In all, 20 kinds of enzyme-encoding genes were related to PSP biosynthesis. The polysaccharide content was positively correlated with the expression patterns of β-fructofuranosidase (sacA), fructokinase (scrK), UDP-glucose 4-epimerase (GALE), Mannose-1-phosphate guanylyltransferase (GMPP), and UDP-glucose 6-dehydrogenase (UGDH), but negatively correlated with the expression of Hexokinase (HK). Through qRT-PCR validation and comprehensive analysis, we determined that sacA, HK, and GMPP are key genes for enzymes within the PSP metabolic pathway in P. sibiricum. Our results provide a public transcriptome dataset for this species and an outline of pathways for the production of polysaccharides in medicinal plants. They also present more information about the PSP biosynthesis pathway at the molecular level in P. sibiricum and lay the foundation for subsequent research of gene functions. Full article

(This article belongs to the Special Issue Molecular Recognition of Carbohydrates)

► Show Figures

Graphical abstract

5520 KiB

Open AccessArticle

Interactions between a Heparin Trisaccharide Library and FGF-1 Analyzed by NMR Methods

by María José García-Jiménez, Sergio Gil-Caballero, Ángeles Canales, Jesús Jiménez-Barbero, José L. De Paz and Pedro M. Nieto

Int. J. Mol. Sci. 2017, 18(6), 1293; https://doi.org/10.3390/ijms18061293 - 17 Jun 2017

Cited by 12 | Viewed by 5318

Abstract

FGF-1 is a potent mitogen that, by interacting simultaneously with Heparan Sulfate Glycosaminoglycan HSGAG and the extracellular domains of its membrane receptor (FGFR), generates an intracellular signal that finally leads to cell division. The overall structure of the ternary complex Heparin:FGF-1:FGFR has been finally elucidated after some controversy and the interactions within the ternary complex have been deeply described. However, since the structure of the ternary complex was described, not much attention has been given to the molecular basis of the interaction between FGF-1 and the HSGAG. It is known that within the complex, the carbohydrate maintains the same helical structure of free heparin that leads to sulfate groups directed towards opposite directions along the molecular axis. The precise role of single individual interactions remains unclear, as sliding and/or rotating of the saccharide along the binding pocket are possibilities difficult to discard. The HSGAG binding pocket can be subdivided into two regions, the main one can accommodate a trisaccharide, while the other binds a disaccharide. We have studied and analyzed the interaction between FGF-1 and a library of trisaccharides by STD-NMR and selective longitudinal relaxation rates. The library of trisaccharides corresponds to the heparin backbone and it has been designed to interact with the main subsite of the protein. Full article

(This article belongs to the Special Issue Molecular Recognition of Carbohydrates)

► Show Figures

Graphical abstract

7256 KiB

Open AccessArticle

Crystallization of Galectin-8 Linker Reveals Intricate Relationship between the N-terminal Tail and the Linker

by Yunlong Si, Yue Wang, Jin Gao, Chenyang Song, Shiqiong Feng, Yifa Zhou, Guihua Tai and Jiyong Su

Int. J. Mol. Sci. 2016, 17(12), 2088; https://doi.org/10.3390/ijms17122088 - 12 Dec 2016

Cited by 24 | Viewed by 5187

Abstract

Galectin-8 (Gal-8) plays a significant role in normal immunological function as well as in cancer. This lectin contains two carbohydrate recognition domains (CRD) connected by a peptide linker. The N-terminal CRD determines ligand binding specificity, whereas the linker has been proposed to regulate overall Gal-8 function, including multimerization and biological activity. Here, we crystallized the Gal-8 N-terminal CRD with the peptide linker using a crystallization condition that contains Ni²⁺. The Ni²⁺ ion was found to be complexed between two CRDs via crystal packing contacts. The coordination between Ni²⁺ and Asp25 plays an indirect role in determining the structure of β-strand F0 and in influencing the linker conformation which could not be defined due to its dynamic nature. The linker was also shortened in situ and crystallized under a different condition, leading to a higher resolution structure refined to 1.08 Å. This crystal structure allowed definition of a short portion of the linker interacting with the Gal-8 N-terminal tail via ionic interactions and hydrogen bonds. Observation of two Gal-8 N-terminal CRD structures implies that the N-terminal tail and the linker may influence each other’s conformation. In addition, under specific crystallization conditions, glycerol could replace lactose and was observed at the carbohydrate binding site. However, glycerol did not show inhibition activity in hemagglutination assay. Full article

(This article belongs to the Special Issue Molecular Recognition of Carbohydrates)

► Show Figures

Figure 1

Review

Jump to: Research

20 pages, 770 KiB

Open AccessReview

Pathogen-Derived Carbohydrate Recognition in Molluscs Immune Defense

by Weilin Wang, Xiaorui Song, Lingling Wang and Linsheng Song

Int. J. Mol. Sci. 2018, 19(3), 721; https://doi.org/10.3390/ijms19030721 - 3 Mar 2018

Cited by 76 | Viewed by 7290

Abstract

Self-nonself discrimination is a common theme for all of the organisms in different evolutionary branches, which is also the most fundamental step for host immune protection. Plenty of pattern recognition receptors (PRRs) with great diversity have been identified from different organisms to recognize various pathogen-associated molecular patterns (PAMPs) in the last two decades, depicting a complicated scene of host-pathogen interaction. However, the detailed mechanism of the complicate PAMPs–PRRs interactions at the contacting interface between pathogens and hosts is still not well understood. All of the cells are coated by glycosylation complex and thick carbohydrates layer. The different polysaccharides in extracellular matrix of pathogen-host are important for nonself recognition of most organisms. Coincidentally, massive expansion of PRRs, majority of which contain recognition domains of Ig, leucine-rich repeat (LRR), C-type lectin (CTL), C1q and scavenger receptor (SR), have been annotated and identified in invertebrates by screening the available genomic sequence. The phylum Mollusca is one of the largest groups in the animal kingdom with abundant biodiversity providing plenty of solutions about pathogen recognition and immune protection, which might offer a suitable model to figure out the common rules of immune recognition mechanism. The present review summarizes the diverse PRRs and common elements of various PAMPs, especially focusing on the structural and functional characteristics of canonical carbohydrate recognition proteins and some novel proteins functioning in molluscan immune defense system, with the objective to provide new ideas about the immune recognition mechanisms. Full article

(This article belongs to the Special Issue Molecular Recognition of Carbohydrates)

► Show Figures

Figure 1

30 pages, 18158 KiB

Open AccessReview

Substrate Recognition and Specificity of Chitin Deacetylases and Related Family 4 Carbohydrate Esterases

by Hugo Aragunde, Xevi Biarnés and Antoni Planas

Int. J. Mol. Sci. 2018, 19(2), 412; https://doi.org/10.3390/ijms19020412 - 30 Jan 2018

Cited by 59 | Viewed by 7557

Abstract

Carbohydrate esterases family 4 (CE4 enzymes) includes chitin and peptidoglycan deacetylases, acetylxylan esterases, and poly-N-acetylglucosamine deacetylases that act on structural polysaccharides, altering their physicochemical properties, and participating in diverse biological functions. Chitin and peptidoglycan deacetylases are not only involved in cell wall morphogenesis and remodeling in fungi and bacteria, but they are also used by pathogenic microorganisms to evade host defense mechanisms. Likewise, biofilm formation in bacteria requires partial deacetylation of extracellular polysaccharides mediated by poly-N-acetylglucosamine deacetylases. Such biological functions make these enzymes attractive targets for drug design against pathogenic fungi and bacteria. On the other side, acetylxylan esterases deacetylate plant cell wall complex xylans to make them accessible to hydrolases, making them attractive biocatalysts for biomass utilization. CE4 family members are metal-dependent hydrolases. They are highly specific for their particular substrates, and show diverse modes of action, exhibiting either processive, multiple attack, or patterned deacetylation mechanisms. However, the determinants of substrate specificity remain poorly understood. Here, we review the current knowledge on the structure, activity, and specificity of CE4 enzymes, focusing on chitin deacetylases and related enzymes active on N-acetylglucosamine-containing oligo and polysaccharides. Full article

(This article belongs to the Special Issue Molecular Recognition of Carbohydrates)

► Show Figures

Graphical abstract

5355 KiB

Open AccessReview

The Role of Carbohydrates in the Lipopolysaccharide (LPS)/Toll-Like Receptor 4 (TLR4) Signalling

by Florent Cochet and Francesco Peri

Int. J. Mol. Sci. 2017, 18(11), 2318; https://doi.org/10.3390/ijms18112318 - 3 Nov 2017

Cited by 129 | Viewed by 14725

Abstract

The interactions between sugar-containing molecules from the bacteria cell wall and pattern recognition receptors (PRR) on the plasma membrane or cytosol of specialized host cells are the first molecular events required for the activation of higher animal’s immune response and inflammation. This review focuses on the role of carbohydrates of bacterial endotoxin (lipopolysaccharide, LPS, lipooligosaccharide, LOS, and lipid A), in the interaction with the host Toll-like receptor 4/myeloid differentiation factor 2 (TLR4/MD-2) complex. The lipid chains and the phosphorylated disaccharide core of lipid A moiety are responsible for the TLR4 agonist action of LPS, and the specific interaction between MD-2, TLR4, and lipid A are key to the formation of the activated complex (TLR4/MD-2/LPS)₂, which starts intracellular signalling leading to nuclear factors activation and to production of inflammatory cytokines. Subtle chemical variations in the lipid and sugar parts of lipid A cause dramatic changes in endotoxin activity and are also responsible for the switch from TLR4 agonism to antagonism. While the lipid A pharmacophore has been studied in detail and its structure-activity relationship is known, the contribution of core saccharides 3-deoxy-d-manno-octulosonic acid (Kdo) and heptosyl-2-keto-3-deoxy-octulosonate (Hep) to TLR4/MD-2 binding and activation by LPS and LOS has been investigated less extensively. This review focuses on the role of lipid A, but also of Kdo and Hep sugars in LPS/TLR4 signalling. Full article

(This article belongs to the Special Issue Molecular Recognition of Carbohydrates)

► Show Figures

Journal Menu

Journal Browser

Molecular Recognition of Carbohydrates

Share This Special Issue

Special Issue Editors

Special Issue Information

Keywords

Benefits of Publishing in a Special Issue

Published Papers (9 papers)

Research

Review

Further Information

Guidelines

MDPI Initiatives

Follow MDPI