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New Plant Models

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Plant Sciences".

Deadline for manuscript submissions: closed (31 January 2021) | Viewed by 8187

Special Issue Editor


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Guest Editor
Department of Biology and Skidmore Microscopy Imaging Center, Skidmore College, Saratoga Springs, NY 12866, USA
Interests: plant cell biology; cell walls; phycology

Special Issue Information

Dear Colleagues,

The recent explosion of research activities that has encompassed a broad taxonomic array of organisms has yielded a new and exciting set of model organisms for future research. This, in turn, will allow for robust investigations that will significantly aid in our understanding of evolutionary relationships, provide convenient organismal systems for elucidating the foundational principles of life, and identify new organisms that will revolutionize applied biology in areas such as agriculture, industry, and medicine. It is the goal of this Special Issue to introduce some of these novel organisms, summarize their unique features, and highlight their future applications in all areas of biological research.

Dr. David Domozych
Guest Editor

Manuscript Submission Information

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Keywords

  • novel model organisms
  • molecular biology
  • cell biology

Published Papers (2 papers)

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Research

25 pages, 5760 KiB  
Article
An Optimized Transformation System and Functional Test of CYC-Like TCP Gene CpCYC in Chirita pumila (Gesneriaceae)
by Jing Liu, Juan-Juan Wang, Jie Wu, Yang Wang, Qi Liu, Fang-Pu Liu, Xia Yang and Yin-Zheng Wang
Int. J. Mol. Sci. 2021, 22(9), 4544; https://doi.org/10.3390/ijms22094544 - 27 Apr 2021
Cited by 5 | Viewed by 2279
Abstract
The development of an ideal model plant located at a key phylogenetic node is critically important to advance functional and regulatory studies of key regulatory genes in the evolutionary developmental (evo-devo) biology field. In this study, we selected Chirita pumila in the family [...] Read more.
The development of an ideal model plant located at a key phylogenetic node is critically important to advance functional and regulatory studies of key regulatory genes in the evolutionary developmental (evo-devo) biology field. In this study, we selected Chirita pumila in the family Gesneriaceae, a basal group in Lamiales, as a model plant to optimize its genetic transformation system established previously by us through investigating a series of factors and further conduct functional test of the CYC-like floral symmetry gene CpCYC. By transforming a RNAi:CpCYC vector, we successfully achieved the desired phenotypes of upright actinomorphic flowers, which suggest that CpCYC actually determines the establishment of floral zygomorphy and the horizontal orientation of flowers in C. pumila. We also confirmed the activities of CpCYC promoter in dorsal petals, dorsal/lateral staminodes, as well as the pedicel by transferring a CpCYC promoter:GUS vector into C. pumila. Furthermore, we testified the availability of a transient gene expression system using C. pumila mesophyll protoplasts. The improved transformation system together with the inherent biological features would make C. pumila an attractive new model in functional and regulatory studies for a broad range of evo-devo issues. Full article
(This article belongs to the Special Issue New Plant Models)
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21 pages, 4733 KiB  
Article
Flowering and Seed Production across the Lemnaceae
by Paul Fourounjian, Janet Slovin and Joachim Messing
Int. J. Mol. Sci. 2021, 22(5), 2733; https://doi.org/10.3390/ijms22052733 - 8 Mar 2021
Cited by 12 | Viewed by 5418
Abstract
Plants in the family Lemnaceae are aquatic monocots and the smallest, simplest, and fastest growing angiosperms. Their small size, the smallest family member is 0.5 mm and the largest is 2.0 cm, as well as their diverse morphologies make these plants ideal for [...] Read more.
Plants in the family Lemnaceae are aquatic monocots and the smallest, simplest, and fastest growing angiosperms. Their small size, the smallest family member is 0.5 mm and the largest is 2.0 cm, as well as their diverse morphologies make these plants ideal for laboratory studies. Their rapid growth rate is partially due to the family’s neotenous lifestyle, where instead of maturing and producing flowers, the plants remain in a juvenile state and continuously bud asexually. Maturation and flowering in the wild are rare in most family members. To promote further research on these unique plants, we have optimized laboratory flowering protocols for 3 of the 5 genera: Spirodela; Lemna; and Wolffia in the Lemnaceae. Duckweeds were widely used in the past for research on flowering, hormone and amino acid biosynthesis, the photosynthetic apparatus, and phytoremediation due to their aqueous lifestyle and ease of aseptic culture. There is a recent renaissance in interest in growing these plants as non-lignified biomass sources for fuel production, and as a resource-efficient complete protein source. The genome sequences of several Lemnaceae family members have become available, providing a foundation for genetic improvement of these plants as crops. The protocols for maximizing flowering described herein are based on screens testing daylength, a variety of media, supplementation with salicylic acid or ethylenediamine-N,N′-bis(2-hydroxyphenylacetic acid) (EDDHA), as well as various culture vessels for effects on flowering of verified Lemnaceae strains available from the Rutgers Duckweed Stock Cooperative. Full article
(This article belongs to the Special Issue New Plant Models)
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