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Advances in Stem Cell Research for Future Therapies and In Vitro Disease Modelling 2.0

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Pathology, Diagnostics, and Therapeutics".

Deadline for manuscript submissions: closed (30 November 2022) | Viewed by 3202

Special Issue Editor


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Guest Editor
Department of Molecular Medicine and Surgery, Karolinska Institutet, Stockholm, Sweden
Interests: regenerative medicine; stem cells; organoids; cell replacement therapies; neurodegeneration; neural stem cells; cardiomyocytes; retinal disorders
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Special Issue Information

Dear Colleagues,

In recent years, stem cell therapy has become a very promising and advanced scientific research topic that is now undergoing its first clinical trials. This growing interest goes hand in hand with industrial partners aiming to bring technology and capacity up to scale and offer cell replacement therapies to patients suffering from chronic disorders.

This Special Issue is focused on recent discoveries that might contribute to the areas of basic stem cell research, in vitro disease modeling, translational stem cell research, and stem cell therapies. Recent advances in cell reprogramming and genome editing technologies have created new possibilities for the development of new in vitro models to study human diseases. More recently, organoids have emerged as powerful tools to better study early developmental biology as a possible source for future cell therapies.

Cell replacement therapies are now entering a new phase of growth, and novel discoveries for cell differentiation, expansion, purification, and delivery under GMP conditions are key aspects to move stem cell products from bench to bedside.

We invite you to contribute to this Special Issue and to communicate new advances in stem cell research for future therapies and in vitro disease modeling with original articles and reviews.

Dr. J. Carlos Villaescusa
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. There is an Article Processing Charge (APC) for publication in this open access journal. For details about the APC please see here. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • embryonic stem cells
  • induced pluripotent stem cells
  • organoids
  • 3D cultures
  • organogenesis
  • in vitro disease modelling
  • developmental biology
  • cell fate
  • bioreactors
  • reprogramming
  • pluripotency
  • production and scalability
  • GMP adaptation
  • cell–material interaction
  • biomaterials
  • regenerative medicine
  • cell therapy
  • 3D scaffold
  • epigenetics

Published Papers (1 paper)

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Research

22 pages, 6973 KiB  
Article
Progesterone Receptor Modulates Extraembryonic Mesoderm and Cardiac Progenitor Specification during Mouse Gastrulation
by Anna Maria Drozd, Luca Mariani, Xiaogang Guo, Victor Goitea, Niels Alvaro Menezes and Elisabetta Ferretti
Int. J. Mol. Sci. 2022, 23(18), 10307; https://doi.org/10.3390/ijms231810307 - 7 Sep 2022
Cited by 1 | Viewed by 2862
Abstract
Progesterone treatment is commonly employed to promote and support pregnancy. While maternal tissues are the main progesterone targets in humans and mice, its receptor (PGR) is expressed in the murine embryo, questioning its function during embryonic development. Progesterone has been previously associated with [...] Read more.
Progesterone treatment is commonly employed to promote and support pregnancy. While maternal tissues are the main progesterone targets in humans and mice, its receptor (PGR) is expressed in the murine embryo, questioning its function during embryonic development. Progesterone has been previously associated with murine blastocyst development. Whether it contributes to lineage specification is largely unknown. Gastrulation initiates lineage specification and generation of the progenitors contributing to all organs. Cells passing through the primitive streak (PS) will give rise to the mesoderm and endoderm. Cells emerging posteriorly will form the extraembryonic mesodermal tissues supporting embryonic growth. Cells arising anteriorly will contribute to the embryonic heart in two sets of distinct progenitors, first (FHF) and second heart field (SHF). We found that PGR is expressed in a posterior–anterior gradient in the PS of gastrulating embryos. We established in vitro differentiation systems inducing posterior (extraembryonic) and anterior (cardiac) mesoderm to unravel PGR function. We discovered that PGR specifically modulates extraembryonic and cardiac mesoderm. Overexpression experiments revealed that PGR safeguards cardiac differentiation, blocking premature SHF progenitor specification and sustaining the FHF progenitor pool. This role of PGR in heart development indicates that progesterone administration should be closely monitored in potential early-pregnancy patients undergoing infertility treatment. Full article
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