Recent Advances in the Understanding of Molecular Mechanisms of Resistance in Lepidopteran Pests: 2nd Edition

A special issue of Insects (ISSN 2075-4450). This special issue belongs to the section "Insect Molecular Biology and Genomics".

Deadline for manuscript submissions: 1 November 2024 | Viewed by 2628

Special Issue Editors


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Guest Editor
Université Côte d’Azur, INRAE, CNRS, ISA, F-06903 Sophia Antipolis, France
Interests: insecticide resistance; insect functional genomic; cytochrome P450; plant-insect interaction
Special Issues, Collections and Topics in MDPI journals
Bayer AG, Crop Science Division, R & D, Pest Control, 40789 Monheim, Germany
Interests: insecticide resistance; insect toxicogenomics; insecticide mode of action; functional genomics; cytochrome P450
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Three years after the first edition, we are pleased to launch a new Special Issue named Recent Advances in the Understanding of Molecular Mechanisms of Resistance in Noctuid Pests. It is an opportunity to focus on the mechanisms of insecticide resistance developed by lepidopterans belonging to the family of Noctuidae (moth species), which are still among the most devastating crop pests on the planet. Some of these noctuids are able to feed on more than 40 different plant families and have a high invasive potential. An example is the fall armyworm, Spodoptera frugiperda, which originated in the American continent and has, in recent years, invaded various regions of the world (Africa, Asia, and Australia). Its distribution area is growing and it is on the doorstep of Europe. In addition to attacking a wide range of crops, such as maize and rice, it has developed resistance to many classes of insecticides and Bacillus thuringiensis (B.t.) pore-forming Cry toxins. Other close species of the genus Spodoptera, such as S. litura, S. littoralis, and S. exigua, have the same adaptive capacities. Another important Noctuid subfamily consists of the heliothine moths, e.g., cotton bollworm, Helicoverpa armigera, a highly polyphagous species, resistant to many insecticides, which has spread from Europe, Asia, Africa, and Australia to South America. Understanding how these insects become resistant to chemical insecticides and B.t. toxins is essential for sustainable control and appropriate resistance management tactics. With the advent of the genomes of these noctuids in recent years, the development of techniques such as CRISPR/Cas9 and GWAS has considerably improved the identification and validation of molecular mechanisms underlying insecticide resistance.

We invite colleagues working on noctuid moth pests that have developed resistance to chemical insecticides and B.t. toxins to submit original papers, short communications, or reviews. Studies may focus on resistance mechanisms based on target-site mutations and/or metabolic detoxification. Genome comparison analyses between these species are also welcome.

Dr. Gaelle Le Goff
Dr. Ralf Nauen
Guest Editors

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Keywords

  • lepidopteran
  • insecticide resistance
  • B.t. resistance
  • mechanisms of resistance
  • resistance management
  • detoxification enzymes
  • insecticide target

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Published Papers (2 papers)

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Research

10 pages, 981 KiB  
Article
V1848I Mutation in the Voltage-Gated Sodium Channel Confers High-Level Resistance to Indoxacarb and Metaflumizone in Spodoptera exigua
by Xiangjie Liu, Minhui Cao, Wenjuan Mei, Xingliang Wang and Yidong Wu
Insects 2024, 15(10), 777; https://doi.org/10.3390/insects15100777 - 8 Oct 2024
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Abstract
Spodoptera exigua is one of the most serious lepidopteran pests of global importance. With the intensive use of insecticides, S. exigua has evolved resistance to many insecticides, including the sodium channel blocker insecticides (SCBIs) indoxacarb and metaflumizone. In this study, we investigated the [...] Read more.
Spodoptera exigua is one of the most serious lepidopteran pests of global importance. With the intensive use of insecticides, S. exigua has evolved resistance to many insecticides, including the sodium channel blocker insecticides (SCBIs) indoxacarb and metaflumizone. In this study, we investigated the role of the V1848I mutation in the voltage-gated sodium channel (VGSC) in SCBI resistance and its inheritance patterns in S. exigua through the development and characterization of a near-isogenic resistant strain. The AQ-23 strain of S. exigua, collected in 2023 from Anqing, Anhui province of China, shows 165-fold resistance to indoxacarb compared with the susceptible WH-S strain. A frequency of 44.6% for the V1848I mutation was detected in the SeVGSC of the AQ-23 strain, while no F1845Y mutation was found. Through repeated backcrossing and marker-assisted selection, the V1848I mutation in the AQ-23 strain was introgressed into the susceptible WH-S strain, creating a near-isogenic strain named WH-1848I. This WH-1848I strain exhibits high levels of resistance to indoxacarb (146-fold) and metaflumizone (431-fold) but remains susceptible to broflanilide and spinosad compared with the WH-S strain. Inheritance analysis revealed that SCBI resistance in the WH-1848I strain is autosomal, nonrecessive, and genetically linked to the V1848I mutation. These findings establish a clear link between the V1848I mutation and SCBI resistance in S. exigua, offering valuable insights for developing molecular detection tools and resistance management strategies. Full article
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19 pages, 7369 KiB  
Article
Genome-Wide Exploration of Long Non-Coding RNAs of Helicoverpa armigera in Response to Pyrethroid Insecticide Resistance
by Md-Mafizur Rahman, Celso Omoto and Juil Kim
Insects 2024, 15(3), 146; https://doi.org/10.3390/insects15030146 - 21 Feb 2024
Cited by 3 | Viewed by 1529
Abstract
Genome-wide long non-coding RNAs (lncRNAs) in low, moderate, and high pyrethroid insecticide-resistant and -susceptible strains of Helicoverpa armigera were identified in this study. Using 45 illumina-based RNA-sequencing datasets, 8394 lncRNAs were identified. In addition, a sublethal dose of deltamethrin was administered to a [...] Read more.
Genome-wide long non-coding RNAs (lncRNAs) in low, moderate, and high pyrethroid insecticide-resistant and -susceptible strains of Helicoverpa armigera were identified in this study. Using 45 illumina-based RNA-sequencing datasets, 8394 lncRNAs were identified. In addition, a sublethal dose of deltamethrin was administered to a Korean-resistant strain (Kor-T). The average length of lncRNAs was approximately 531 bp, and the expression ratio of lncRNAs was 28% of the total RNA. The identified lncRNAs were divided into six categories—intronic, intergenic, sense, antisense, cis-RNA, and trans-RNA—based on their location and mechanism of action. Intergenic and intronic lncRNA transcripts were the most abundant (38% and 33%, respectively). Further, 828 detoxification-related lncRNAs were selected using the Gene Ontology analysis. The cytochrome P450-related lncRNA expression levels were significantly higher in susceptible strains than in resistant strains. In contrast, cuticle protein-related lncRNA expression levels were significantly higher in all resistant strains than in susceptible strains. Our findings suggest that certain lncRNAs contribute to the downregulation of insecticide resistance-related P450 genes in susceptible strains, whereas other lncRNAs may be involved in the overexpression of cuticle protein genes, potentially affecting the pyrethroid resistance mechanism. Full article
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