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J. Imaging
Special Issues
In-vivo Imaging
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In-vivo Imaging

A special issue of Journal of Imaging (ISSN 2313-433X).

Deadline for manuscript submissions: closed (28 February 2019)

Special Issue Editors

Prof. Dr. Laura Sironi

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Guest Editor
Physics Department, University of Milano-Bicocca, Milan, Italy
Interests: gold nanoparticles; bioapplication; biosensors; fluorescence imaging
Dr. Donato Inverso

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Guest Editor
Division of Vascular Oncology and Metastasis, German Cancer Research Center (Deutsches Krebsforschungszentrum, DKFZ), lm Neuenheimer Feld 280, 69120 Heidelberg, Germany
Interests: in-vivo imaging; liver phatology; hemodynamic; vascular biology; immune cells traffiking; confocal microscopy; 3D modelling

Special Issue Information

Dear Colleagues,

In vivo imaging paved a new path in the study of biological processes since it allows the real-time 3D visualization of cellular and molecular dynamic processes directly in living organisms. Among other in vivo imaging techniques, such as positron emission tomography (PET), single photon emission computed tomography (SPECT), computed tomography (CT) and magnetic resonance imaging (MRI), optical intravital imaging has recently become an effective tool to address tissue architecture, hemodynamics, cell migration and intracellular processes at high temporal and spatial resolution. Transgenic approaches and, more recently, advances in nanomaterials play a pivotal role in visualizing processes at the molecular level and in understanding the pathophysiology of diseases. Moreover, novel image analysis algorithms combined also with adaptive optical methods allowed further advance in the quantitative information that we can extract from images to highlight pathologies.

The scope of this Special Issue is to provide both review and original manuscripts regarding the following areas: 1) novel preclinical applications of in vivo imaging techniques, with particular attention to optical microscopy (confocal/multi-photon excitation microscopy, single plane illumination microscopy, fluorescence/bioluminescence imaging, whole-body optical imaging); 2) new insights in the development of innovative techniques and image-analysis methods for studying tissue physiology and pathology in real time; and 3) novel tools for functional and molecular imaging

This Special Issue is intended to cover the following topics, but not limited to them:

  • Innovative in vivo imaging set-ups (development, validation and applications), including adaptive optics
  • Applications of single or multiphoton excitation fluorescence microscopy for intravital imaging
  • Advanced image-analysis algorithms and tools for in-vivo microscopy
  • In vivo imaging for real-time monitoring of diseases and pathologies
  • In vivo super-resolution microscopy
  • In vivo microscopy for real-time tissue structure monitoring or functional and molecular imaging
  • Animal models and novel fluorescence markers (including the use of nanoparticles for targeted molecular imaging and diagnostic)
  • Combination of optical microscopy with CT/MRI/PET/SPECT

Dr. Laura Sironi
Dr. Inverso Donato
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Journal of Imaging is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1800 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • optical microscopy (fluorescence and bioluminescence),
  • single/multi-photon intravital microscopy,
  • single-plane illumination microscopy (SPIM),
  • in-vivo super-resolution microscopy
  • adaptive optics,
  • machine learning-based algorithms
  • image analysis,
  • nanoparticles,
  • functional imaging,
  • molecular imaging,
  • hemodynamic parameters measurements,
  • tissue architecture parameters,
  • in-vivo monitoring of diseases and pathologies
  • animal models
  • liver pathology,
  • infectious disease
  • vascular biology
  • immune cells trafficking
  • single/multi-photon intravital microscopy,
  • molecular biology

Published Papers (2 papers)

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24 pages, 42148 KiB  
Article
Real-Time In Vivo Imaging of the Developing Pupal Wing Tissues in the Pale Grass Blue Butterfly Zizeeria maha: Establishing the Lycaenid System for Multiscale Bioimaging
by Kanako Hirata and Joji M. Otaki
J. Imaging 2019, 5(4), 42; https://doi.org/10.3390/jimaging5040042 - 28 Mar 2019
Cited by 8 | Viewed by 7475
Abstract
To systematically analyze biological changes with spatiotemporal dynamics, it is important to establish a system that is amenable for real-time in vivo imaging at various size levels. Herein, we focused on the developing pupal wing tissues in the pale grass blue butterfly, Zizeeria [...] Read more.
To systematically analyze biological changes with spatiotemporal dynamics, it is important to establish a system that is amenable for real-time in vivo imaging at various size levels. Herein, we focused on the developing pupal wing tissues in the pale grass blue butterfly, Zizeeria maha, as a system of choice for a systematic multiscale approach in vivo in real time. We showed that the entire pupal wing could be monitored throughout development using a high-resolution bright-field time-lapse imaging system under the forewing-lift configuration; we recorded detailed dynamics of the dorsal and ventral epithelia that behaved independently for peripheral adjustment. We also monitored changes in the dorsal hindwing at the compartmental level and directly observed evaginating scale buds. We also employed a confocal laser microscopy system with multiple fluorescent dyes for three-dimensional observations at the tissue and cellular levels. We discovered extensive cellular clusters that may be functionally important as a unit of cellular communication and differentiation. We also identified epithelial discal and marginal dents that may function during development. Together, this lycaenid forewing system established a foundation to study the differentiation process of epithelial cells and can be used to study biophysically challenging mechanisms such as the determination of color patterns and scale nanoarchitecture at the multiscale levels. Full article
(This article belongs to the Special Issue In-vivo Imaging)
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13 pages, 3518 KiB  
Article
Understanding Vasomotion of Lung Microcirculation by In Vivo Imaging
by Enrico Mazzuca, Andrea Aliverti and Giuseppe Miserocchi
J. Imaging 2019, 5(2), 22; https://doi.org/10.3390/jimaging5020022 - 22 Jan 2019
Cited by 9 | Viewed by 5609
Abstract
The balance of lung extravascular water depends upon the control of blood flow in the alveolar distribution vessels that feed downstream two districts placed in parallel, the corner vessels and the alveolar septal network. The occurrence of an edemagenic condition appears critical as [...] Read more.
The balance of lung extravascular water depends upon the control of blood flow in the alveolar distribution vessels that feed downstream two districts placed in parallel, the corner vessels and the alveolar septal network. The occurrence of an edemagenic condition appears critical as an increase in extravascular water endangers the thinness of the air–blood barrier, thus negatively affecting the diffusive capacity of the lung. We exposed anesthetized rabbits to an edemagenic factor (12% hypoxia) for 120 min and followed by in vivo imaging the micro-vascular morphology through a “pleural window” using a stereo microscope at a magnification of 15× (resolution of 7.2 μm). We measured the change in diameter of distribution vessels (50–200 μm) and corner vessels (<50 μm). On average, hypoxia caused a significant decrease in diameter of both smaller distribution vessels (about ~50%) and corner vessels (about ~25%) at 30 min. After 120 min, reperfusion occurred. Regional differences in perivascular interstitial volume were observed and could be correlated with differences in blood flow control. To understand such difference, we modelled imaged alveolar capillary units, obtained by Voronoi method, integrating microvascular pressure parameters with capillary filtration. Results of the analysis suggested that at 120 min, alveolar blood flow was diverted to the corner vessels in larger alveoli, which were found also to undergo a greater filtration indicating greater proneness to develop lung edema. Full article
(This article belongs to the Special Issue In-vivo Imaging)
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