Molecular Biology of Mushroom

A special issue of Journal of Fungi (ISSN 2309-608X). This special issue belongs to the section "Fungal Genomics, Genetics and Molecular Biology".

Deadline for manuscript submissions: 31 March 2025 | Viewed by 639

Special Issue Editor


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Guest Editor
Department of Medicinal Biosciences, Research Institute for Biomedicinal & Health Science, College of Biomedicinal and Health Science, Konkuk University, Chungju 27478, Republic of Korea
Interests: fungal genomics

Special Issue Information

Dear Colleagues,

Mushrooms have long been used as food and medicinal ingredients and are an excellent model for biological research.  However, many mushrooms' basic biology and genetics are poorly understood. Fortunately, with the development of various molecular biology technologies, the biological properties of mushrooms that were not understood until now are gradually being revealed, and the various uses of mushrooms are attracting attention. Recently, Omics-based research on various mushrooms has been actively conducted, and the veil on mushrooms is gradually lifted. Through this, the biological characteristics of mushrooms and their genetic characteristics are being revealed. In addition, many mushrooms produce toxins, and their medical uses are also receiving attention. 

In this Special Issue, we invite you to participate in introducing the latest achievements in the field of molecular biology of mushrooms. This will not only be helpful to researchers in various fields researching mushrooms but will also contribute to the development of related research fields.

Dr. Young-Jin Park
Guest Editor

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Keywords

  • molecular biology
  • genetics
  • genomics
  • transcriptomics
  • medicinal uses
  • metabolism and metabolites
  • evolution
  • fruiting body development
  • genome editing
  • phylogeny

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Published Papers (1 paper)

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Research

20 pages, 17936 KiB  
Article
Genome Sequencing Providing Molecular Evidence of Tetrapolar Mating System and Heterothallic Life Cycle for Edible and Medicinal Mushroom Polyporus umbellatus Fr.
by Shoujian Li, Youyan Liu, Liu Liu, Bing Li and Shunxing Guo
J. Fungi 2025, 11(1), 15; https://doi.org/10.3390/jof11010015 - 28 Dec 2024
Viewed by 429
Abstract
Polyporus umbellatus is a species whose sclerotia have been extensively employed in traditional Chinese medicine, which has diuretic, antitumor, anticancer, and immune system enhancement properties. However, prolonged asexual reproduction has resulted in significant homogenization and degeneration of seed sclerotia. In contrast, sexual reproduction [...] Read more.
Polyporus umbellatus is a species whose sclerotia have been extensively employed in traditional Chinese medicine, which has diuretic, antitumor, anticancer, and immune system enhancement properties. However, prolonged asexual reproduction has resulted in significant homogenization and degeneration of seed sclerotia. In contrast, sexual reproduction has emerged as an effective strategy to address these challenges, with a distinct mating system serving as the foundation for the implementation of sexual breeding. This study presents the first sequencing and assembly of the genome of P. umbellatus, thereby providing an opportunity to investigate the mating system at the genomic level. Based on the annotated mating-type loci within the genome, monokaryotic offspring exhibiting different mating-types were identified. Through the integration of traditional mating tests, the tetrapolar mating system of P. umbellatus was distinctly elucidated. The resequencing of monokaryotic strains with four different mating-types, along with comparative analyses of mating-type loci, revealed the HD1 and HD2 (HD, homeodomain) genes determined the mating A types, and the PR4, PR5, and PR6 (PR, pheromone receptor) genes determined the mating B types. Meanwhile, this study offers a successful case study in the molecular investigation of mating systems. Additionally, the number of sterigma and basidiospores on each basidium was examined using scanning electron microscopy, while the nuclei of basidiospores and basidia at various developmental stages were analyzed through DAPI staining. This research clarifies the heterothallic life cycle of P. umbellatus. The findings of this study are expected to facilitate advancements in genetic research, breeding development, strain improvement, and the industry of P. umbellatus. Full article
(This article belongs to the Special Issue Molecular Biology of Mushroom)
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