Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
9.6
4.9
Journals
Marine Drugs
Special Issues
3D Cell Culture Based on Marine Resources
share announcement

3D Cell Culture Based on Marine Resources

A special issue of Marine Drugs (ISSN 1660-3397).

Deadline for manuscript submissions: closed (20 December 2020) | Viewed by 39764

Special Issue Editor

Prof. Dr. Jong-Young Kwak

E-Mail Website
Guest Editor
1. Department of Pharmacology, School of Medicine, Ajou University, Worldcup-Ro 164, Suwon 16499, Republic of Korea
2. 3D Immune System Imaging Core Center, Ajou University, Suwon 16499, Republic of Korea
3. Immune-Network Pioneer Research Center, Ajou University Medical Center, Suwon 16499, Republic of Korea
Interests: 3D culture; immune microenvironment; functional polymer
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Three-dimensional (3D) cell culture leads to more predictive models for drug discovery and tissue regeneration. An ideal 3D culture model has not yet been defined due to the loss of extracellular matrix-like scaffolds, although various 3D cell culture model systems require such scaffolds for 3D cell growth. Marine origin polymers have been used as scaffolds for 3D cell culture and will be developed as biomaterials for bio-ink in bioprinting. Thus, many polymers such as alginates, carrageenans, fucoidans, and chitosans from marine resources, including fish, algae, crustaceans, bacteria, cyanobacteria, actinobacteria, and fungi, are considered to be promising biomaterials for 3D cell and tissue culture. In addition, the biological assays of bio-active materials from marine resources using 3D culture rather than two-dimensional (2D) culture have been expanded. This Special Issue aims to provide an overview of the current research in 3D culture systems employing marine natural products as biomaterials. Innovative 3D culture systems to assay marine drugs are also one of the focuses of this Special Issue. As Guest Editor of this Special Issue of Marine Drugs, I cordially invite contributions in the form of original research articles or reviews on the subject of this advancing research field.

Prof. Dr. Jong-Young Kwak
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Marine Drugs is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2900 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • 3D cell culture
  • 3D scaffold
  • polymer
  • marine resources
  • biomaterials
  • 3D assay

Benefits of Publishing in a Special Issue

  • Ease of navigation: Grouping papers by topic helps scholars navigate broad scope journals more efficiently.
  • Greater discoverability: Special Issues support the reach and impact of scientific research. Articles in Special Issues are more discoverable and cited more frequently.
  • Expansion of research network: Special Issues facilitate connections among authors, fostering scientific collaborations.
  • External promotion: Articles in Special Issues are often promoted through the journal's social media, increasing their visibility.
  • e-Book format: Special Issues with more than 10 articles can be published as dedicated e-books, ensuring wide and rapid dissemination.

Further information on MDPI's Special Issue polices can be found here.

Published Papers (5 papers)

Download All Papers
Order results
Result details
Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:

Research

Jump to: Review

19 pages, 3973 KiB  
Article
The Incorporation of Marine Coral Microparticles into Collagen-Based Scaffolds Promotes Osteogenesis of Human Mesenchymal Stromal Cells via Calcium Ion Signalling
by Eamon J. Sheehy, Mark Lemoine, Declan Clarke, Arlyng Gonzalez Vazquez and Fergal J. O’Brien
Mar. Drugs 2020, 18(2), 74; https://doi.org/10.3390/md18020074 - 23 Jan 2020
Cited by 15 | Viewed by 3648
Abstract
Composite biomaterial scaffolds consisting of natural polymers and bioceramics may offer an alternative to autologous grafts for applications such as bone repair. Herein, we sought to investigate the possibility of incorporating marine coral microparticles into a collagen-based scaffold, a process which we hypothesised [...] Read more.
Composite biomaterial scaffolds consisting of natural polymers and bioceramics may offer an alternative to autologous grafts for applications such as bone repair. Herein, we sought to investigate the possibility of incorporating marine coral microparticles into a collagen-based scaffold, a process which we hypothesised would enhance the mechanical properties of the scaffold as well its capacity to promote osteogenesis of human mesenchymal stromal cells. Cryomilling and sieving were utilised to achieve coral microparticles of mean diameters 14 µm and 64 µm which were separately incorporated into collagen-based slurries and freeze-dried to form porous scaffolds. X-ray diffraction and Fourier transform infrared spectroscopy determined the coral microparticles to be comprised of calcium carbonate whereas collagen/coral composite scaffolds were shown to have a crystalline calcium ethanoate structure. Crosslinked collagen/coral scaffolds demonstrated enhanced compressive properties when compared to collagen only scaffolds and also promoted more robust osteogenic differentiation of mesenchymal stromal cells, as indicated by increased expression of bone morphogenetic protein 2 at the gene level, and enhanced alkaline phosphatase activity and calcium accumulation at the protein level. Only subtle differences were observed when comparing the effect of coral microparticles of different sizes, with improved osteogenesis occurring as a result of calcium ion signalling delivered from collagen/coral composite scaffolds. These scaffolds, fabricated from entirely natural sources, therefore show promise as novel biomaterials for tissue engineering applications such as bone regeneration. Full article
(This article belongs to the Special Issue 3D Cell Culture Based on Marine Resources)
►▼ Show Figures

Graphical abstract

12 pages, 4721 KiB  
Article
Plocabulin Displays Strong Cytotoxic Activity in a Personalized Colon Cancer Patient-Derived 3D Organoid Assay
by Alba Costales-Carrera, Asunción Fernández-Barral, Pilar Bustamante-Madrid, Laura Guerra, Ramón Cantero, Antonio Barbáchano and Alberto Muñoz
Mar. Drugs 2019, 17(11), 648; https://doi.org/10.3390/md17110648 - 19 Nov 2019
Cited by 32 | Viewed by 4515
Abstract
Plocabulin is a novel microtubule-disrupting antitumor agent of marine origin that is currently undergoing phase II clinical trials. Plocabulin has potent antiproliferative and antiangiogenic actions in carcinoma cell lines and has antitumor activity in xenografted mice. Here, we used three-dimensional (3D) tumor organoids [...] Read more.
Plocabulin is a novel microtubule-disrupting antitumor agent of marine origin that is currently undergoing phase II clinical trials. Plocabulin has potent antiproliferative and antiangiogenic actions in carcinoma cell lines and has antitumor activity in xenografted mice. Here, we used three-dimensional (3D) tumor organoids derived from three colorectal cancer (CRC) patients to study the effect of plocabulin in a personalized assay system that ensures dose dependence and high reproducibility. The cytotoxicity of plocabulin was an order of magnitude higher than that of the active irinotecan derivative SN38 (7-ethyl-10-hydroxy-camptothecin) in tumor organoids at different passages. Moreover, plocabulin maintained its strong cytotoxic activity in wash-out experiments, in which a short pulse treatment of tumor organoids was as efficient as continuous treatment. Our data show that plocabulin has a very potent cytotoxic action in CRC patient-derived tumor organoids, supporting ongoing clinical trials with plocabulin and the use of organoid assays to provide personalized validation of antitumor drugs. Full article
(This article belongs to the Special Issue 3D Cell Culture Based on Marine Resources)
►▼ Show Figures

Graphical abstract

Review

Jump to: Research

37 pages, 5000 KiB  
Review
Biomaterials Based on Marine Resources for 3D Bioprinting Applications
by Yi Zhang, Dezhi Zhou, Jianwei Chen, Xiuxiu Zhang, Xinda Li, Wenxiang Zhao and Tao Xu
Mar. Drugs 2019, 17(10), 555; https://doi.org/10.3390/md17100555 - 28 Sep 2019
Cited by 60 | Viewed by 8651
Abstract
Three-dimensional (3D) bioprinting has become a flexible tool in regenerative medicine with potential for various applications. Further development of the new 3D bioprinting field lies in suitable bioink materials with satisfied printability, mechanical integrity, and biocompatibility. Natural polymers from marine resources have been [...] Read more.
Three-dimensional (3D) bioprinting has become a flexible tool in regenerative medicine with potential for various applications. Further development of the new 3D bioprinting field lies in suitable bioink materials with satisfied printability, mechanical integrity, and biocompatibility. Natural polymers from marine resources have been attracting increasing attention in recent years, as they are biologically active and abundant when comparing to polymers from other resources. This review focuses on research and applications of marine biomaterials for 3D bioprinting. Special attention is paid to the mechanisms, material requirements, and applications of commonly used 3D bioprinting technologies based on marine-derived resources. Commonly used marine materials for 3D bioprinting including alginate, carrageenan, chitosan, hyaluronic acid, collagen, and gelatin are also discussed, especially in regards to their advantages and applications. Full article
(This article belongs to the Special Issue 3D Cell Culture Based on Marine Resources)
►▼ Show Figures

Figure 1

16 pages, 725 KiB  
Review
Current Stage of Marine Ceramic Grafts for 3D Bone Tissue Regeneration
by Patricia Diaz-Rodriguez, Miriam López-Álvarez, Julia Serra, Pío González and Mariana Landín
Mar. Drugs 2019, 17(8), 471; https://doi.org/10.3390/md17080471 - 15 Aug 2019
Cited by 23 | Viewed by 5672
Abstract
Bioceramic scaffolds are crucial in tissue engineering for bone regeneration. They usually provide hierarchical porosity, bioactivity, and mechanical support supplying osteoconductive properties and allowing for 3D cell culture. In the case of age-related diseases such as osteoarthritis and osteoporosis, or other bone alterations [...] Read more.
Bioceramic scaffolds are crucial in tissue engineering for bone regeneration. They usually provide hierarchical porosity, bioactivity, and mechanical support supplying osteoconductive properties and allowing for 3D cell culture. In the case of age-related diseases such as osteoarthritis and osteoporosis, or other bone alterations as alveolar bone resorption or spinal fractures, functional tissue recovery usually requires the use of grafts. These bone grafts or bone void fillers are usually based on porous calcium phosphate grains which, once disposed into the bone defect, act as scaffolds by incorporating, to their own porosity, the intergranular one. Despite their routine use in traumatology and dental applications, specific graft requirements such as osteoinductivity or balanced dissolution rate are still not completely fulfilled. Marine origin bioceramics research opens the possibility to find new sources of bone grafts given the wide diversity of marine materials still largely unexplored. The interest in this field has also been urged by the limitations of synthetic or mammalian-derived grafts already in use and broadly investigated. The present review covers the current stage of major marine origin bioceramic grafts for bone tissue regeneration and their promising properties. Both products already available on the market and those in preclinical phases are included. To understand their clear contribution to the field, the main clinical requirements and the current available biological-derived ceramic grafts with their advantages and limitations have been collected. Full article
(This article belongs to the Special Issue 3D Cell Culture Based on Marine Resources)
►▼ Show Figures

Graphical abstract

32 pages, 1968 KiB  
Review
Marine Collagen as A Promising Biomaterial for Biomedical Applications
by Ye-Seon Lim, Ye-Jin Ok, Seon-Yeong Hwang, Jong-Young Kwak and Sik Yoon
Mar. Drugs 2019, 17(8), 467; https://doi.org/10.3390/md17080467 - 10 Aug 2019
Cited by 205 | Viewed by 16365
Abstract
This review focuses on the expanding role of marine collagen (MC)-based scaffolds for biomedical applications. A scaffold—a three-dimensional (3D) structure fabricated from biomaterials—is a key supporting element for cell attachment, growth, and maintenance in 3D cell culture and tissue engineering. The mechanical and [...] Read more.
This review focuses on the expanding role of marine collagen (MC)-based scaffolds for biomedical applications. A scaffold—a three-dimensional (3D) structure fabricated from biomaterials—is a key supporting element for cell attachment, growth, and maintenance in 3D cell culture and tissue engineering. The mechanical and biological properties of the scaffolds influence cell morphology, behavior, and function. MC, collagen derived from marine organisms, offers advantages over mammalian collagen due to its biocompatibility, biodegradability, easy extractability, water solubility, safety, low immunogenicity, and low production costs. In recent years, the use of MC as an increasingly valuable scaffold biomaterial has drawn considerable attention from biomedical researchers. The characteristics, isolation, physical, and biochemical properties of MC are discussed as an understanding of MC in optimizing the subsequent modification and the chemistries behind important tissue engineering applications. The latest technologies behind scaffold processing are assessed and the biomedical applications of MC and MC-based scaffolds, including tissue engineering and regeneration, wound dressing, drug delivery, and therapeutic approach for diseases, especially those associated with metabolic disturbances such as obesity and diabetes, are discussed. Despite all the challenges, MC holds great promise as a biomaterial for developing medical products and therapeutics. Full article
(This article belongs to the Special Issue 3D Cell Culture Based on Marine Resources)
►▼ Show Figures

Figure 1

Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:
 
Displaying articles 1-5
Back to TopTop