Lateral Flow Assays

A special issue of Micromachines (ISSN 2072-666X). This special issue belongs to the section "B4: Point-of-Care Devices".

Deadline for manuscript submissions: closed (28 February 2023) | Viewed by 2047

Special Issue Editor


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Guest Editor
Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 410049 Saratov, Russia
Interests: gold nanoparticles; plasmon resonance; composite nanoparticles; functionalization of nanoparticles; conjugates; lateral flow immunoassay; gold–silver nanocages; Raman spectroscopy

Special Issue Information

Dear Colleagues,

Lateral flow immunoassay (LFIA) is a promising method of analysis in our changing world. Recently, LFIA has played a role in the development of methods for the rapid detection of certain substances in point of care. LFIA is based on a specific antigen–antibody interaction. The test strip is placed in the analyzed liquid and by the appearance of staining at the place of labeling, the presence or absence of the desired substance in the analyzed liquid is checked. Due to its simplicity and speed of achievement of the result, LFIA has taken its place among reliable analytical methods. Due to its versatility, immunochromatographic analysis is used to identify pathogens and related antibodies, pesticides, herbicides, antibiotics in food, and much more. However, despite the more than sixty-year history of the existence of immunochromatographic analysis, many aspects of its work remain unclear, which gives scope for fundamental and applied research in this area. In this Special Issue, we plan to collect articles on the development and application of immunochromatographic analysis for developments at the forefront of modern science.

Dr. Elizaveta Panfilova
Guest Editor

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Keywords

  • lateral flow immunoassay
  • rapid detection
  • gold nanoparticles conjugation
  • point-of-care testing
  • immunochromatographic test

Published Papers (1 paper)

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Research

15 pages, 2384 KiB  
Article
Double Immunochromatographic Test System for Sensitive Detection of Phycotoxins Domoic Acid and Okadaic Acid in Seawater and Seafood
by Olga D. Hendrickson, Elena A. Zvereva, Olga N. Solopova, Anatoly V. Zherdev, Peter G. Sveshnikov, Sergei A. Eremin and Boris B. Dzantiev
Micromachines 2022, 13(9), 1506; https://doi.org/10.3390/mi13091506 - 10 Sep 2022
Cited by 7 | Viewed by 1580
Abstract
In this investigation, a double immunochromatographic analysis (ICA) of two relevant phycotoxins, domoic acid (DA) and okadaic acid (OA), was developed for the first time. The ICA was performed in the indirect competitive format using gold nanoparticles conjugated with anti-species antibodies. Under optimal [...] Read more.
In this investigation, a double immunochromatographic analysis (ICA) of two relevant phycotoxins, domoic acid (DA) and okadaic acid (OA), was developed for the first time. The ICA was performed in the indirect competitive format using gold nanoparticles conjugated with anti-species antibodies. Under optimal conditions, the instrumental detection limits/cutoffs for simultaneous detection of DA and OA were 1.2/100 and 0.1/2.5 ng/mL, respectively. The time of the assay was 18 min. The ICA was applied to test seawater and a large panel of seafood, including mussels, tiger shrimps, octopuses, whelks, crabs, and scallops. The proposed simple sample preparation method for seafood takes only 20 min. For seawater, a dilution by buffer was implemented. The assay recoveries varied from 80.8% to 124.5%. The competitive potential of the proposed technique as a tool to control natural water and seafood samples is determined by its simplicity, rapidity, and sensitivity. Full article
(This article belongs to the Special Issue Lateral Flow Assays)
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