Escherichia coli and Food Safety 2.0

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Food Microbiology".

Deadline for manuscript submissions: closed (15 December 2023) | Viewed by 3509

Special Issue Editor


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Guest Editor
Department of Food, Environment and Nutrition-DeFENS, University of Milan, Via Celoria 2, I-20133 Milano, Italy
Interests: food microbiology; food safety; foodborne pathogens; fermented foods; bacteriophages; lactic acid bacteria
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Special Issue Information

Dear Colleagues,

This Special Issue is a continuation of our 2021 Special Issue "Escherichia coli and Food Safety".

Escherichia coli is a common member of microflora of humans and other mammals. However, these microorganisms can acquire virulence factors from the exchange of mobile genetic elements, such as transposons, insertion sequences, bacteriophages, and plasmids. These factors are linked to the emergence of pathogenic strains (such as Shiga-toxin-producing E. coli, STEC) with enhanced survival and persistence in food systems. Pathogenic E. coli can cause mild to severe diseases in humans when ingested through contaminated foods and water.

Contamination can occur all along the food chain, from production to preparation, and the contamination path can include all aspects of human, animal, and plant interfaces, as well as their interactions with the ecosystem. Therefore, it is fundamental to ensure an adequate food safety program and develop new methods to counteract the presence and the development of pathogenic E. coli.

This Special Issue will cover research and review articles focused on the control of pathogenic E. coli in the food supply chain, as well as on the selection of intervention strategies to reduce the presence of these microorganisms, with a special focus on bacteriocins, natural antimicrobials, bacteriophages, or any other biopreservative agents.

Dr. Claudia Picozzi
Guest Editor

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Keywords

  • pathogenic E. coli
  • STEC/VTEC
  • food safety
  • food contamination
  • prevention and control
  • intervention strategies
  • risk assessment

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Published Papers (2 papers)

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Research

20 pages, 3289 KiB  
Article
Dynamic Interactions between Diarrhoeagenic Enteroaggregative Escherichia coli and Presumptive Probiotic Bacteria: Implications for Gastrointestinal Health
by Wisdom Selorm Kofi Agbemavor and Elna Maria Buys
Microorganisms 2023, 11(12), 2942; https://doi.org/10.3390/microorganisms11122942 - 8 Dec 2023
Cited by 1 | Viewed by 1012
Abstract
This study delves into the temporal dynamics of bacterial interactions in the gastrointestinal tract, focusing on how probiotic strains and pathogenic bacteria influence each other and human health. This research explores adhesion, competitive exclusion, displacement, and inhibition of selected diarrhoeagenic Escherichia coli (D-EAEC) [...] Read more.
This study delves into the temporal dynamics of bacterial interactions in the gastrointestinal tract, focusing on how probiotic strains and pathogenic bacteria influence each other and human health. This research explores adhesion, competitive exclusion, displacement, and inhibition of selected diarrhoeagenic Escherichia coli (D-EAEC) and potential probiotic strains under various conditions. Key findings reveal that adhesion is time-dependent, with both D-EAEC K2 and probiotic L. plantarum FS2 showing increased adhesion over time. Surprisingly, L. plantarum FS2 outperformed D-EAEC K2 in adhesion and exhibited competitive exclusion and displacement, with inhibition of adhesion surpassing competitive exclusion. This highlights probiotics’ potential to slow pathogen attachment when not in competition. Pre-infecting with L. plantarum FS2 before pathogenic infection effectively inhibited adhesion, indicating probiotics’ ability to prevent pathogen attachment. Additionally, adhesion correlated strongly with interleukin-8 (IL-8) secretion, linking it to the host’s inflammatory response. Conversely, IL-8 secretion negatively correlated with trans-epithelial electrical resistance (TEER), suggesting a connection between tight junction disruption and increased inflammation. These insights offer valuable knowledge about the temporal dynamics of gut bacteria interactions and highlight probiotics’ potential in competitive exclusion and inhibiting pathogenic bacteria, contributing to strategies for maintaining gastrointestinal health and preventing infections. Full article
(This article belongs to the Special Issue Escherichia coli and Food Safety 2.0)
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15 pages, 4350 KiB  
Article
Genome-Based Characterization of Hybrid Shiga Toxin-Producing and Enterotoxigenic Escherichia coli (STEC/ETEC) Strains Isolated in South Korea, 2016–2020
by Woojung Lee, Min-Hee Kim, Soohyun Sung, Eiseul Kim, Eun Sook An, Seung Hwan Kim, Soon Han Kim and Hae-Yeong Kim
Microorganisms 2023, 11(5), 1285; https://doi.org/10.3390/microorganisms11051285 - 15 May 2023
Cited by 9 | Viewed by 1647
Abstract
The global emergence of hybrid diarrheagenic E. coli strains incorporating genetic markers from different pathotypes is a public health concern. Hybrids of Shiga toxin-producing and enterotoxigenic E. coli (STEC/ETEC) are associated with diarrhea and hemolytic uremic syndrome (HUS) in humans. In this study, [...] Read more.
The global emergence of hybrid diarrheagenic E. coli strains incorporating genetic markers from different pathotypes is a public health concern. Hybrids of Shiga toxin-producing and enterotoxigenic E. coli (STEC/ETEC) are associated with diarrhea and hemolytic uremic syndrome (HUS) in humans. In this study, we identified and characterized STEC/ETEC hybrid strains isolated from livestock feces (cattle and pigs) and animal food sources (beef, pork, and meat patties) in South Korea between 2016 and 2020. The strains were positive for genes from STEC and ETEC, such as stx (encodes Shiga toxins, Stxs) and est (encodes heat-stable enterotoxins, ST), respectively. The strains belong to diverse serogroups (O100, O168, O8, O155, O2, O141, O148, and O174) and sequence types (ST446, ST1021, ST21, ST74, ST785, ST670, ST1780, ST1782, ST10, and ST726). Genome-wide phylogenetic analysis revealed that these hybrids were closely related to certain ETEC and STEC strains, implying the potential acquisition of Stx-phage and/or ETEC virulence genes during the emergence of STEC/ETEC hybrids. Particularly, STEC/ETEC strains isolated from livestock feces and animal source foods mostly exhibited close relatedness with ETEC strains. These findings allow further exploration of the pathogenicity and virulence of STEC/ETEC hybrid strains and may serve as a data source for future comparative studies in evolutionary biology. Full article
(This article belongs to the Special Issue Escherichia coli and Food Safety 2.0)
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