Effect of Bacterial Biofilms Colonization on Oral Health and Therapy 2.0

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Biofilm".

Deadline for manuscript submissions: closed (30 November 2023) | Viewed by 544

Special Issue Editor

1. Department of Endodontics, Maurice and Gabriela Goldschleger School of Dental Medicine, Tel Aviv University, Tel Aviv 6997801, Israel
2. Center for Nanoscience and Nanotechnology, Tel Aviv University, Tel Aviv 6997801, Israel
Interests: endodontic microbiology; nanoscience; nanotechnology
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

A bacterial biofilm is a common ecological community, where bacteria are held together by an extracellular matrix, and use different mechanisms to align their activity within the community and to achieve complex multi-cellular activities. Biofilms enable bacteria to attach more firmly to their hosts and to have better access to nutrients. When compared to the planktonic (free-living) state, bacteria in biofilms are more protected from environmental insults, antibiotics, and the immune system. Biofilm colonization represents most of the microbial infections in the human body including in the oral cavity, where biofilms are the main source of many oral diseases, such as dental abscesses, periodontal diseases, and dental caries. These colonized oral biofilms pose a significant clinical challenge as they may resist common therapies and produce persistent infections.

The aims of the Special Issue are to present up-to-date evidence-based data regarding the colonization of bacterial biofilms in the different niches of the oral cavity and their involvement in oral diseases, and to present novel therapeutic approaches to overcome these persistent infections.

Dr. Eyal Rosen
Guest Editor

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Keywords

  • biofilm
  • bacterial colonization
  • oral microbiota
  • bacterial resistance
  • persistent infection
  • endodontic biofilms
  • periodontal biofilms
  • prosthodontics and oral biofilms
  • novel methods to eradicate oral biofilms

Published Papers (1 paper)

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Research

13 pages, 2134 KiB  
Article
Establishment of a Protocol for Viability qPCR in Dental Hard Tissues
by Torsten Sterzenbach, Vanessa Neumann, Evelyn Trips, Sabine Basche, Christian Hannig and Marie-Theres Kühne
Microorganisms 2024, 12(7), 1400; https://doi.org/10.3390/microorganisms12071400 - 11 Jul 2024
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Abstract
The aim of the study was to establish a live/dead qPCR with propidium monoazide (PMA) that can quantitatively differentiate between viable/non-viable microorganisms in dental hard tissues. Human premolars (n = 88) were prepared with nickel–titanium instruments and incubated with E. faecalis (21 d). [...] Read more.
The aim of the study was to establish a live/dead qPCR with propidium monoazide (PMA) that can quantitatively differentiate between viable/non-viable microorganisms in dental hard tissues. Human premolars (n = 88) were prepared with nickel–titanium instruments and incubated with E. faecalis (21 d). Subsequently, the bacteria in half of the teeth were devitalized by heat inactivation (100 °C, 2 h). The following parameters were tested: PMA concentrations at 0 µmol (control), 50 µmol, 100 µmol, and 200 µmol; PMA incubation times of 30 min and 60 min, and blue light treatment for 30 min and 60 min. The teeth were ground using a cryomill and the bacterial DNA was quantified using qPCR, ANOVA, and p = 0.05. The qPCR of the control group detected a similar number of avital 9.94 × 106 and vital 1.61 × 107 bacterial cells. The use of PMA inhibited the amplification of DNA from non-viable cells during qPCR. As a result, the best detection of avital bacteria was achieved with the following PMA parameters: (concentration, incubation time, blue light treatment) 200-30-30; 5.53 × 104 (avital) and 1.21 × 100.7 (vital). The live/dead qPCR method using PMA treatment is suitable for the differentiation and quantification of viable/non-viable microorganisms in dentin, as well as to evaluate the effectiveness of different preparation procedures and antimicrobial irrigants in other biological hard substances. Full article
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