Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
7.4
4.1
Journals
Microorganisms
Special Issues
Innovative Approaches to Fight Infectious Diseases
share announcement

Innovative Approaches to Fight Infectious Diseases

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Antimicrobial Agents and Resistance".

Deadline for manuscript submissions: closed (30 November 2021) | Viewed by 3071

Special Issue Editor

Dr. Philippe Brouqui

E-Mail Website
Guest Editor
IHU mediterranée Infection, AP-HM Assistance Publique - Hôpitaux de Marseille, Marseille, France
Interests: emerging infection; innovative technology; public health; clinical microbiology; outbreak preparedness; hospital acquired infection; human behavior; COVID-19

Special Issue Information

Dear Colleagues,

The major lesson of COVID 19 is that infectious diseases are unpredictable and that their understanding lacks knowledge. The quick mutation of RNA viruses, highlighted by the analysis of complete genome sequences; understanding microbe transmission; the need to improve prevention and public health measures, tracking contagious patients; and the need for quickly available treatments suggest a need for innovative organizational, technological, preventive, and therapeutical approaches. This issue aims to report on all these aspects of the fight against infectious diseases such as drug repurposing, innovative diagnostics and therapeutic approaches, and tracking and monitoring technology. These may or may not be related to COVID-19 but may be related to innovative approaches such as microbiota transplantation, repurposing in infection treatment, innovative drug-delivery systems for persistent infections, and behavioral approaches to understanding transmission. All innovative approaches related to microbiology, technological, fundamental, behavioral, and epidemiological, are welcome.

Dr. Philippe Brouqui
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Microorganisms is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • emerging infection
  • innovative technology
  • public health
  • clinical Microbiology
  • outbreak preparedness
  • hospital acquired infection
  • human behavior
  • COVID-19

Published Papers (1 paper)

Order results
Result details
Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:

Research

10 pages, 8588 KiB  
Article
Innovative Approach to Fast Electron Microscopy Using the Example of a Culture of Virus-Infected Cells: An Application to SARS-CoV-2
by Marion Le Bideau, Nathalie Wurtz, Jean-Pierre Baudoin and Bernard La Scola
Microorganisms 2021, 9(6), 1194; https://doi.org/10.3390/microorganisms9061194 - 31 May 2021
Cited by 6 | Viewed by 2571
Abstract
Despite the development of new diagnostic methods, co-culture, based on sample inoculation of cell monolayers coupled with electron microscopy (EM) observation, remains the gold standard in virology. Indeed, co-culture allows for the study of cell morphology (infected and not infected), the ultrastructure of [...] Read more.
Despite the development of new diagnostic methods, co-culture, based on sample inoculation of cell monolayers coupled with electron microscopy (EM) observation, remains the gold standard in virology. Indeed, co-culture allows for the study of cell morphology (infected and not infected), the ultrastructure of the inoculated virus, and the different steps of the virus infectious cycle. Most EM methods for studying virus cycles are applied after infected cells are produced in large quantities and detached to obtain a pellet. Here, cell culture was performed in sterilized, collagen-coated single-break strip wells. After one day in culture, cells were infected with SARS-CoV-2. Wells of interest were fixed at different time points, from 2 to 36 h post-infection. Microwave-assisted resin embedding was accomplished directly in the wells in 4 h. Finally, ultra-thin sections were cut directly through the infected-cell monolayers. Our methodology requires, in total, less than four days for preparing and observing cells. Furthermore, by observing undetached infected cell monolayers, we were able to observe new ultrastructural findings, such as cell–cell interactions and baso-apical cellular organization related to the virus infectious cycle. Our innovative methodology thus not only saves time for preparation but also adds precision and new knowledge about viral infection, as shown here for SARS-CoV-2. Full article
(This article belongs to the Special Issue Innovative Approaches to Fight Infectious Diseases)
►▼ Show Figures

Figure 1

Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:
 
Displaying articles 1-1
Back to TopTop