Yeast Production of Flavours and Bioactive Molecules in Fermented Beverages

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Microbial Biotechnology".

Deadline for manuscript submissions: closed (30 April 2021) | Viewed by 2883

Special Issue Editors


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UMR SPO, Université Montpellier, INRAE, Montpellier SupAgro, 34060 Montpellier, France
Interests: fermentation biotechnology; Microbial Biotechnology; Food Science and Technology; food quality; applied microbiology; yeasts metabolism and physiology; wine microbiology

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South African Grape and Wine Research Institute, Stellenbosch University, Matieland 7602, South Africa
Interests: fermentation; wine microbiology; wine biotechnology; yeasts physiology and biochemistry

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Instituto de Agroquímica y Tecnología de los Alimentos (IATA-CSIC), 46980 Paterna, Valencia, Spain
Interests: wine yeasts; wine fermentation
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Special Issue Information

Dear Colleagues,

Currently, offering high-quality products that stand out in an increasingly competitive global market while meeting the demands of environmentally friendly practices is the main challenge faced by the fermented beverage industries. Innovation is essential to address these issues, in particular through the development of new or improved sustainable technologies and processes aiming to control/diversify the production of compounds contributing to the quality of the final products (wine, cider, beer, spirits, etc.). Among these molecules, volatile compounds, also referred to as flavour or aroma compounds, and bioactive molecules are produced during alcoholic fermentation by the principal fermenting agent, Saccharomyces cerevisiae, but also increasingly by non-Saccharomyces yeasts occurring in these biotechnological habitats. In recent years, particular attention has been paid to the identification of both the specific properties of non-conventional species and the formation of non-conventional molecules, thus opening up a wide field for fundamental and applied research, many aspects of which remain to be explored.

In this context, this Special Issue of Microorganisms aims to give the opportunity to researchers to present their most recent work and to exchange views, experiences, and updates on the latest advances on the production of flavours and bioactive molecules by yeasts during alcoholic fermentation and the different ways to control/modulate it, including in particular the exploitation of yeast diversity and the management of the process.

For this purpose, we invite you to submit original research articles, review articles, and short communications related to any aspects related to yeast phenotypic and genomic diversity, metabolism and its regulation, identification of the molecular basis underlying specific traits, yeast nutrition, interactions between yeast species and between yeast and environment in the context of the alcoholic beverage production.

Dr. Carole Camarasa
Dr. Benoit Divol
Dr. José Manuel Guillamón
Guest Editors

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Keywords

  • production of flavours and bioactive molecules
  • alcoholic fermentation
  • yeast metabolism and physiology
  • yeast diversity in alcoholic beverage environments

Published Papers (1 paper)

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Research

17 pages, 3323 KiB  
Article
Does Saccharomyces cerevisiae Require Specific Post-Translational Silencing against Leaky Translation of Hac1up?
by Ali Tehfe, Talia Roseshter, Yulong Wei and Xuhua Xia
Microorganisms 2021, 9(3), 620; https://doi.org/10.3390/microorganisms9030620 - 17 Mar 2021
Cited by 1 | Viewed by 2465
Abstract
HAC1 encodes a key transcription factor that transmits the unfolded protein response (UPR) from the endoplasmic reticulum (ER) to the nucleus and regulates downstream UPR genes in Saccharomyces cerevisiae. In response to the accumulation of unfolded proteins in the ER, Ire1p oligomers [...] Read more.
HAC1 encodes a key transcription factor that transmits the unfolded protein response (UPR) from the endoplasmic reticulum (ER) to the nucleus and regulates downstream UPR genes in Saccharomyces cerevisiae. In response to the accumulation of unfolded proteins in the ER, Ire1p oligomers splice HAC1 pre-mRNA (HAC1u) via a non-conventional process and allow the spliced HAC1 (HAC1i) to be translated efficiently. However, leaky splicing and translation of HAC1u may occur in non-UPR cells to induce undesirable UPR. To control accidental UPR activation, multiple fail-safe mechanisms have been proposed to prevent leaky HAC1 splicing and translation and to facilitate rapid degradation of translated Hac1up and Hac1ip. Among proposed regulatory mechanisms is a degron sequence encoded at the 5′ end of the HAC1 intron that silences Hac1up expression. To investigate the necessity of an intron-encoded degron sequence that specifically targets Hac1up for degradation, we employed publicly available transcriptomic data to quantify leaky HAC1 splicing and translation in UPR-induced and non-UPR cells. As expected, we found that HAC1u is only efficiently spliced into HAC1i and efficiently translated into Hac1ip in UPR-induced cells. However, our analysis of ribosome profiling data confirmed frequent occurrence of leaky translation of HAC1u regardless of UPR induction, demonstrating the inability of translation fail-safe to completely inhibit Hac1up production. Additionally, among 32 yeast HAC1 surveyed, the degron sequence is highly conserved by Saccharomyces yeast but is poorly conserved by all other yeast species. Nevertheless, the degron sequence is the most conserved HAC1 intron segment in yeasts. These results suggest that the degron sequence may indeed play an important role in mitigating the accumulation of Hac1up to prevent accidental UPR activation in the Saccharomyces yeast. Full article
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