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Microorganisms
Special Issues
The Role of Calcium in Bacteria
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The Role of Calcium in Bacteria

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Molecular Microbiology and Immunology".

Deadline for manuscript submissions: closed (30 November 2021) | Viewed by 7581

Special Issue Editors

Prof. Dr. Delfina C. Dominguez

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Guest Editor
Clinical Laboratory Science Program/Department of Public Health Sciences, The University of Texas at El Paso, El Paso, TX, USA
Interests: antibiotic
Dr. Marianna Patrauchan

E-Mail Website
Guest Editor
Department of Microbiology and Molecular Genetics, Oklahoma State University, Stillwater, OK 74078, USA
Interests: calcium-regulated antibiotic resistance; molecular mechanisms of PolymyxinB resistance; Pseudomonas aeruginosa; calcium-induced tobramycin resistance; calcium signaling antimicrobial peptides; biofilm resistance

Special Issue Information

Dear Colleagues,

The calcium ion (Ca2+) has been recognized as a key messenger and regulator in eukaryotic cells. Ca2+ signaling is well understood in mammals and regulates many vital processes ranging from fertilization to cell death. Many physiological responses depend on transient changes in intracellular Ca2+ concentrations, which are utilized by cells to transmit information. However, in prokaryotes, our understanding of the molecular mechanisms of cytosolic Ca2+ transients and Ca2+ signaling is very limited. Evidence that Ca2+ plays an important role in prokaryotic cells is growing. Ca2+ ions are implicated in a wide variety of bacterial processes, including cell differentiation, chemotaxis, transport, gene expression and virulence. Similar to eukaryotes, Ca2+-binding proteins have been identified, cytosolic Ca2+ transients occur in response to stimuli, and cytosolic Ca2+ homeostasis has been demonstrated in prokaryotes. It is crucial to understand the role of Ca2+ in bacterial cells, as this will impact bacterial pathogenesis, design of new therapeutics and further our knowledge in microbial physiology. We welcome original research papers or review articles on the role of Ca2+ in bacteria related but not limited to: transport mechanisms, host pathogen interactions, chemotaxis, infection, virulence, cell cycle, homeostasis, biofilm formation, Two-component regulatory systems, and Ca2+-binding proteins.

Prof. Dr. Delfina C. Dominguez
Dr. Marianna Patrauchan
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Microorganisms is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Ca2+ signaling
  • Ca2+ transport
  • Ca2+binding proteins
  • Ca2+ homeostasis
  • Ca2+ mediated regulation

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Published Papers (1 paper)

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Research

18 pages, 2919 KiB  
Article
Machine Learning Establishes Single-Cell Calcium Dynamics as an Early Indicator of Antibiotic Response
by Christian T. Meyer, Megan P. Jewell, Eugene J. Miller and Joel M. Kralj
Microorganisms 2021, 9(5), 1000; https://doi.org/10.3390/microorganisms9051000 - 5 May 2021
Cited by 8 | Viewed by 3553
Abstract
Changes in bacterial physiology necessarily precede cell death in response to antibiotics. Herein we investigate the early disruption of Ca2+ homeostasis as a marker for antibiotic response. Using a machine learning framework, we quantify the temporal information encoded in single-cell Ca2+ [...] Read more.
Changes in bacterial physiology necessarily precede cell death in response to antibiotics. Herein we investigate the early disruption of Ca2+ homeostasis as a marker for antibiotic response. Using a machine learning framework, we quantify the temporal information encoded in single-cell Ca2+ dynamics. We find Ca2+ dynamics distinguish kanamycin sensitive and resistant cells before changes in gross cell phenotypes such as cell growth or protein stability. The onset time (pharmacokinetics) and probability (pharmacodynamics) of these aberrant Ca2+ dynamics are dose and time-dependent, even at the resolution of single-cells. Of the compounds profiled, we find Ca2+ dynamics are also an indicator of Polymyxin B activity. In Polymyxin B treated cells, we find aberrant Ca2+ dynamics precedes the entry of propidium iodide marking membrane permeabilization. Additionally, we find modifying membrane voltage and external Ca2+ concentration alters the time between these aberrant dynamics and membrane breakdown suggesting a previously unappreciated role of Ca2+ in the membrane destabilization during Polymyxin B treatment. In conclusion, leveraging live, single-cell, Ca2+ imaging coupled with machine learning, we have demonstrated the discriminative capacity of Ca2+ dynamics in identifying antibiotic-resistant bacteria. Full article
(This article belongs to the Special Issue The Role of Calcium in Bacteria)
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