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Molecules
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Emerging Materials, Probe, Fundamental, Strategy for Advanced Chemical Sensor
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Emerging Materials, Probe, Fundamental, Strategy for Advanced Chemical Sensor

A special issue of Molecules (ISSN 1420-3049). This special issue belongs to the section "Chemical Biology".

Deadline for manuscript submissions: closed (15 January 2023)

Special Issue Editors

Dr. Xia Li

E-Mail Website
Guest Editor
Department of Chemistry, Liaocheng University, Liaocheng, 252059, Shandong, China
Interests: DNA sensor; signal amplification; cell imaging
Prof. Wei Li

E-Mail Website
Guest Editor
College of Pharmaceutical Science, Hebei University, Baoding, 071002, China
Interests: nanowalker; multiplexed sensing; microRNA

Special Issue Information

Dear Colleagues,

Advanced chemical sensors involve emerging materials, probes, novel concepts, rational designs, fundamentals, and strategies in chemistry, bioscience, and photonics. Significant advances in these aspects can make chemical sensors more sensitive, rapid, accessible, and intelligent. Topics of interest for creative explorations and investigations include novel concepts, materials, devices, design, and detection mechanisms of sensors based on chemistry, including but not limited to:

  • Chemical sensor construction with novel materials, structures, and performance;
  • Progress in fabrication technologies or devices of chemical sensors;
  • Facile chemical sensors with different signal amplification, transduction, and output strategies;
  • Synthesis, characterization, and chemical sensing applications of emerging 1D, 2D, and 3D nanomaterials;
  • Illustration of fundamental phenomena related to chemical sensors;
  • State of the development of different chemical sensors;
  • Emerging methodologies used in chemical, electrochemical, or biochemical sensing.

Dr. Xia Li
Prof. Wei Li
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Molecules is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • advanced chemical sensor
  • emerging materials
  • probe
  • fundamentals
  • strategy

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Published Papers (1 paper)

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Research

11 pages, 2484 KiB  
Article
Self-Supplying Guide RNA-Mediated CRISPR/Cas12a Fluorescence System for Sensitive Detection of T4 PNKP
by Xiuhua Yuan, Hui Yuan, Bingxin Liu and Yeling Liu
Molecules 2022, 27(24), 9019; https://doi.org/10.3390/molecules27249019 - 17 Dec 2022
Cited by 1 | Viewed by 2168
Abstract
Sensitive detection methods for T4 polynucleotide kinase/phosphatase (T4 PNKPP) are urgently required to obtain information on malignancy and thereby to provide better guidance in PNKP-related diagnostics and drug screening. Although the CRISPR/Cas12a system shows great promise in DNA-based signal amplification protocols, its guide [...] Read more.
Sensitive detection methods for T4 polynucleotide kinase/phosphatase (T4 PNKPP) are urgently required to obtain information on malignancy and thereby to provide better guidance in PNKP-related diagnostics and drug screening. Although the CRISPR/Cas12a system shows great promise in DNA-based signal amplification protocols, its guide RNAs with small molecular weight often suffer nuclease degradation during storage and utilization, resulting in reduced activation efficiency. Herein, we proposed a self-supplying guide RNA-mediated CRISPR/Cas12a system for the sensitive detection of T4 PNKP in cancer cells, in which multiple copies of guide RNA were generated by in situ transcription. In this assay, T4 PNKP was chosen as a model, and a dsDNA probe with T7 promoter region and the transcription region of guide RNA were involved. Under the action of T4 PNKP, the 5′-hydroxyl group of the dsDNA probe was converted to a phosphate group, which can be recognized and digested by Lambda Exo, resulting in dsDNA hydrolysis. The transcription template was destroyed, which resulted in the failure to generate guide RNA by the transcription pathway. Therefore, the CRISPR/Cas12a system could not be activated to effectively cleavage the F-Q-reporter, and the fluorescence signal was turned off. In the absence of T4 PNKP, the 5′-hydroxyl group of the substrate DNA cannot be digested by Lambda Exo. The intact dsDNA acts as the transcription template to generate a large amount of guide RNA. Finally, the formed Cas12a/gRNA complex triggered the reverse cleavage of Cas12a on the F-Q-reporter, resulting in a “turn-on” fluorescence signal. This strategy displayed sharp sensitivity of T4 PNKP with the limit of detection (LOD) down to 0.0017 mU/mL, which was mainly due to the multiple regulation effect of transcription amplification. In our system, the dsDNA simultaneously serves as the T4 PNKP substrate, transcription template, and Lambda Exo substrate, avoiding the need for multiple probe designs and saving costs. By integrating the target recognition, Lambda Exo activity, and trans-cleavage activity of Cas12a, CRISPR/Cas12a catalyzed the cleavage of fluorescent-labeled short-stranded DNA probes and enabled synergetic signal amplification for sensitive T4 PNKP detection. Furthermore, the T4 PNKP in cancer cells has been evaluated as a powerful tool for biomedical research and clinical diagnosis, proving a good practical application capacity. Full article
(This article belongs to the Special Issue Emerging Materials, Probe, Fundamental, Strategy for Advanced Chemical Sensor)
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