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Electroporation - Drug Delivery and Anticancer Approach
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Electroporation - Drug Delivery and Anticancer Approach

A special issue of Molecules (ISSN 1420-3049). This special issue belongs to the section "Medicinal Chemistry".

Deadline for manuscript submissions: closed (28 February 2022) | Viewed by 8723

Special Issue Editor

Dr. Jolanta Saczko

E-Mail Website
Guest Editor
Department of Molecular and Cellular Biology, Faculty of Pharmacy, Wroclaw Medical University, Borowska street 211A, 50-556 Wroclaw, Poland
Interests: cell death; apoptosis; oxidative stress; anti-cancer new therapy; electrochemotherapy; natural compounds; cell metabolism; drug resistance
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Current anti-cancer therapies are still not effective and have numerous side effects. Researchers are looking for new, more effective, and safe methods for cancer treatment. Physical methods such as electroporation or chemical methods that involve drug modification and encapsulation are widely used in medicine and biotechnology. As a result of these actions, new more effective procedures could be developed to find promising applications in medical and pharmaceutical sciences. This Special Issue will cover the latest research concerning physical and chemical methods implemented in drug delivery and therapy and their therapeutic applications, mainly but not solely applied to cancer therapies.

Dr. Jolanta Saczko
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Molecules is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Cell death
  • Apoptosis
  • Cell cycle
  • Electrochemotherapy
  • Autophagy

Published Papers (3 papers)

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Research

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14 pages, 5228 KiB  
Article
Micro- and Nanosecond Pulses Used in Doxorubicin Electrochemotherapy in Human Breast and Colon Cancer Cells with Drug Resistance
by Nina Rembiałkowska, Vitalij Novickij, Dagmara Baczyńska, Magda Dubińska-Magiera, Jolanta Saczko, Julia Rudno-Rudzińska, Magdalena Maciejewska and Julita Kulbacka
Molecules 2022, 27(7), 2052; https://doi.org/10.3390/molecules27072052 - 22 Mar 2022
Cited by 6 | Viewed by 2209
Abstract
(1) Background: Pulsed electric field (PEF) techniques are commonly used to support the delivery of various molecules. A PEF seems a promising method for low permeability drugs or when cells demonstrate therapy resistance and the cell membrane becomes an impermeable barrier. (2) Methods: [...] Read more.
(1) Background: Pulsed electric field (PEF) techniques are commonly used to support the delivery of various molecules. A PEF seems a promising method for low permeability drugs or when cells demonstrate therapy resistance and the cell membrane becomes an impermeable barrier. (2) Methods: In this study, we have used doxorubicin-resistant and sensitive models of human breast cancer (MCF-7/DX, MCF-7/WT) and colon cancer cells (LoVo, LoVoDX). The study aimed to investigate the susceptibility of the cells to doxorubicin (DOX) and electric fields in the 20–900 ns pulse duration range. The viability assay was utilized to evaluate the PEF protocols’ efficacy. Cell confluency and reduced glutathione were measured after PEF protocols. (3) Results: The obtained results showed that PEFs significantly supported doxorubicin delivery and cytotoxicity after 48 and 72 h. The 60 kV/cm ultrashort pulses × 20 ns × 400 had the most significant cytotoxic anticancer effect. The increase in DOX concentration provokes a decrease in cell viability, affected cell confluency, and reduced GSSH when combined with the ESOPE (European Standard Operating Procedures of Electrochemotherapy) protocol. Additionally, reactive oxygen species after PEF and PEF-DOX were detected. (4) Conclusions: Ultrashort electric pulses with low DOX content or ESOPE with higher DOX content seem the most promising in colon and breast cancer treatment. Full article
(This article belongs to the Special Issue Electroporation - Drug Delivery and Anticancer Approach)
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22 pages, 3650 KiB  
Article
Pulse Duration Dependent Asymmetry in Molecular Transmembrane Transport Due to Electroporation in H9c2 Rat Cardiac Myoblast Cells In Vitro
by Tina Batista Napotnik and Damijan Miklavčič
Molecules 2021, 26(21), 6571; https://doi.org/10.3390/molecules26216571 (registering DOI) - 30 Oct 2021
Cited by 2 | Viewed by 2447
Abstract
Electroporation (EP) is one of the successful physical methods for intracellular drug delivery, which temporarily permeabilizes plasma membrane by exposing cells to electric pulses. Orientation of cells in electric field is important for electroporation and, consequently, for transport of molecules through permeabilized plasma [...] Read more.
Electroporation (EP) is one of the successful physical methods for intracellular drug delivery, which temporarily permeabilizes plasma membrane by exposing cells to electric pulses. Orientation of cells in electric field is important for electroporation and, consequently, for transport of molecules through permeabilized plasma membrane. Uptake of molecules after electroporation are the greatest at poles of cells facing electrodes and is often asymmetrical. However, asymmetry reported was inconsistent and inconclusive—in different reports it was either preferentially anodal or cathodal. We investigated the asymmetry of polar uptake of calcium ions after electroporation with electric pulses of different durations, as the orientation of elongated cells affects electroporation to a different extent when using electric pulses of different durations in the range of 100 ns to 100 µs. The results show that with 1, 10, and 100 µs pulses, the uptake of calcium ions is greater at the pole closer to the cathode than at the pole closer to the anode. With shorter 100 ns pulses, the asymmetry is not observed. A different extent of electroporation at different parts of elongated cells, such as muscle or cardiac cells, may have an impact on electroporation-based treatments such as drug delivery, pulse-field ablation, and gene electrotransfection. Full article
(This article belongs to the Special Issue Electroporation - Drug Delivery and Anticancer Approach)
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Review

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32 pages, 2089 KiB  
Review
Electroporation and Electrochemotherapy in Gynecological and Breast Cancer Treatment
by Zofia Łapińska, Urszula Szwedowicz, Anna Choromańska and Jolanta Saczko
Molecules 2022, 27(8), 2476; https://doi.org/10.3390/molecules27082476 - 12 Apr 2022
Cited by 8 | Viewed by 3298
Abstract
Gynecological carcinomas affect an increasing number of women and are associated with poor prognosis. The gold standard treatment plan is mainly based on surgical resection and subsequent chemotherapy with cisplatin, 5-fluorouracil, anthracyclines, or taxanes. Unfortunately, this treatment is becoming less effective and is [...] Read more.
Gynecological carcinomas affect an increasing number of women and are associated with poor prognosis. The gold standard treatment plan is mainly based on surgical resection and subsequent chemotherapy with cisplatin, 5-fluorouracil, anthracyclines, or taxanes. Unfortunately, this treatment is becoming less effective and is associated with many side effects that negatively affect patients’ physical and mental well-being. Electroporation based on tumor exposure to electric pulses enables reduction in cytotoxic drugs dose while increasing their effectiveness. EP-based treatment methods have received more and more interest in recent years and are the subject of a large number of scientific studies. Some of them show promising therapeutic potential without using any cytotoxic drugs or molecules already present in the human body (e.g., calcium electroporation). This literature review aims to present the fundamental mechanisms responsible for the course of EP-based therapies and the current state of knowledge in the field of their application in the treatment of gynecological neoplasms. Full article
(This article belongs to the Special Issue Electroporation - Drug Delivery and Anticancer Approach)
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