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Advancements in Phytochemical Analysis: Development and Application of Novel Instruments...
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Advancements in Phytochemical Analysis: Development and Application of Novel Instruments and Techniques

A special issue of Molecules (ISSN 1420-3049). This special issue belongs to the section "Natural Products Chemistry".

Deadline for manuscript submissions: closed (30 April 2022) | Viewed by 5587

Special Issue Editors

Prof. Dr. Audrius Maruska

E-Mail Website
Guest Editor
Analysis Open Access Center, Faculty of Natural Sciences, Vytautas Magnus University, LT-44404 Kaunas, Lithuania
Interests: analytical chemistry; miniaturization, integration and automation of analysis; development of separation materials and methods; phytochemical analysis; bioremedition; antioxidants
Special Issues, Collections and Topics in MDPI journals
Prof. Dr. Elz̀bieta Skrzydlewska

E-Mail Website
Guest Editor
Department of Analytical Chemistry, Medical Univesrity of Białystok, Bialystok, Poland
Interests: oxidative stress and its consequences in various pathological conditions in humans (tick diseases, RA, skin diseases, cancer, hypertension); animal models and cell cultures; modification of the structure and function of phospholipids metabolites; proteins involved in metabolic processes
Special Issues, Collections and Topics in MDPI journals
Dr. Mantas Stankevičius

E-Mail Website
Guest Editor
Instrumental Analysis Open Access Center, Faculty of Natural Sciences, Vytautas Magnus University, Kaunas, Lithuania
Interests: analytical chemistry; development of separation methods; capillary electrophoresis; coupling and miniaturization of methods; food analysis; fermentation; biotechnology

Special Issue Information

Dear Colleagues,

Future technologies not only rely on sustainability and renewable resources but also on the development of new techniques and instruments required for modern agro-, bio-, food and pharmaceutical technologies. These are closely related with advancements of techniques for desired molecular production and analytical evaluation required in the industrial process monitoring and quality assessment of raw materials and products. A vital aspect of such technologies deals with phytochemicals; therefore, this Special Issue is devoted to the advancement of the techniques and instruments used for phytochemical analysis that are currently applied in the aforementioned industries and research and quality assessment laboratories. It should also be taken into account that compounds of plant origin, especially biologically active compounds, are increasingly used in pharmacotherapy, which indicates that methodologies for their determination in biological materials, based on modern analytical technologies ensuring low levels of quantification, must be developed.

Prof. Dr. Audrius Maruska
Prof. Dr. Elz̀bieta Skrzydlewska
Dr. Mantas Stankevičius
Guest Editors

Manuscript Submission Information

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Keywords

  • phytochemical analysis
  • biological activity of phytocompounds
  • antioxidant activity
  • radical scavenging activity
  • analytical methods
  • integration of analytical methods

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Published Papers (2 papers)

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Research

13 pages, 1856 KiB  
Article
Analysis of the Leaves and Cones of Lithuanian Hops (Humulus lupulus L.) Varieties by Chromatographic and Spectrophotometric Methods
by Žydrūnas Stanius, Mantas Dūdėnas, Vilma Kaškonienė, Mantas Stankevičius, Elżbieta Skrzydlewska, Tomas Drevinskas, Ona Ragažinskienė, Kęstutis Obelevičius and Audrius Maruška
Molecules 2022, 27(9), 2705; https://doi.org/10.3390/molecules27092705 - 22 Apr 2022
Cited by 4 | Viewed by 2602
Abstract
This work involves a comprehensive chemical composition analysis of leaf and cone samples of Lithuanian hop varieties. This study aimed to determine the chemometric properties of the leaves and cones of five Lithuanian hop varieties. Determined properties were the following: (a) xanthohumol content, [...] Read more.
This work involves a comprehensive chemical composition analysis of leaf and cone samples of Lithuanian hop varieties. This study aimed to determine the chemometric properties of the leaves and cones of five Lithuanian hop varieties. Determined properties were the following: (a) xanthohumol content, (b) phenolic compounds, (c) flavonoids, (d) radical scavenging activity, and (e) the qualitative composition of volatile compounds. The total content of phenolic compounds in aqueous 75% methanolic extracts varied between 31.4–78.2 mg of rutin equivalents (RE)/g, and the concentration of flavonoids was between 11.0–23.3 mg RE/g. Radical scavenging activity varied between 34.4–87.2 mg RE/g. A QUENCHER analysis procedure showed 91.7–168.5 mg RE/g of the total phenolic compound content, 12.7–21.4 mg RE/g of flavonoids, and 48.4–121.0 mg RE/g of radical scavenging activity. ‘Fredos taurieji’ and ‘Fredos derlingieji’ varieties have shown maximum values of phenolic compounds and radical scavenging activity both in leaf and cone suspensions. These varieties accumulated a higher amount of xanthohumol in leaves. The concentration of xanthohumol in the samples varied between 0.0014–0.2136% of dry mass, with the highest concentration in the cones of ‘Kauno gražieji’. We identified 19 volatile compounds in leaves, and in cones, we identified 32. In both of them, α-humulene and β caryophyllene dominated. ‘Raudoniai’ leaves were exceptional in their aroma due to dominating compound nagina ketone (Kovats index 1306). The QUENCHER procedure has shown a great potential for the unextractable residue of hop raw material. Further investigation and valorization of different hop biomass components, not only cones, are essential. Full article
(This article belongs to the Special Issue Advancements in Phytochemical Analysis: Development and Application of Novel Instruments and Techniques)
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15 pages, 13531 KiB  
Article
Carotenoid Contents of Lycium barbarum: A Novel QAMS Analyses, Geographical Origins Discriminant Evaluation, and Storage Stability Assessment
by Ruru Ren, Yanting Li, Huan Chen, Yingli Wang, Lingling Yang, Chao Su, Xiaojun Zhao, Jianyu Chen and Xueqin Ma
Molecules 2021, 26(17), 5374; https://doi.org/10.3390/molecules26175374 - 3 Sep 2021
Cited by 8 | Viewed by 2123
Abstract
Given the standard substances of zeaxanthin and its homologues obtained from Lycium barbarum L. (LB) are extremely scarce and unstable, a novel quantitative analysis of carotenoids by single marker method, named QAMS, was established. Four carotenoids including lutein, zeaxanthin, β-carotene, and zeaxanthin dipalmitate [...] Read more.
Given the standard substances of zeaxanthin and its homologues obtained from Lycium barbarum L. (LB) are extremely scarce and unstable, a novel quantitative analysis of carotenoids by single marker method, named QAMS, was established. Four carotenoids including lutein, zeaxanthin, β-carotene, and zeaxanthin dipalmitate were determined simultaneously by employing trans-β-apo-8′-carotenal, a carotenoid component which did not exist in LB, as standard reference. Meanwhile, β-carotene, another carotenoid constituent which existed in LB, was determined as contrast. The QAMS methods were fully verified and exhibited low standard method difference with the external standard method (ESM), evidenced by the contents of four carotenoids in 34 batches of LB samples determined using ESM and QAMS methods, respectively. HCA, PCA, and OPLS-DA analysis disclosed that LB samples could be clearly differentiated into two groups: one contained LB samples collected from Ningxia and Gansu; the other was from Qinghai, which was directly related to the different geographical location. Once exposed under high humidity (RH 75 ± 5%) at a high temperature (45 ± 5 °C) as compared with ambient temperature (25 ± 5 °C), from day 0 to day 28, zeaxanthin dipalmitate content was significantly decreased, and ultimately, all the decrease rates reached about 80%, regardless of the storage condition. Our results provide a good basis for improving the quality control of LB. Full article
(This article belongs to the Special Issue Advancements in Phytochemical Analysis: Development and Application of Novel Instruments and Techniques)
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