Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
7.4
4.2
Journals
Molecules
Special Issues
High-throughput Screening
molecules-logo
Submit to Molecules Review for Molecules Propose a Special Issue

Journal Menu

► Journal Menu

Journal Browser

► Journal Browser
share announcement

High-throughput Screening

A special issue of Molecules (ISSN 1420-3049). This special issue belongs to the section "Molecular Diversity".

Deadline for manuscript submissions: closed (15 January 2010) | Viewed by 95742

Special Issue Editor

Dr. Florian Hollfelder

E-Mail Website
Guest Editor
Department of Biochemistry, 80 Tennis Court Road, 3.84 Sanger Building, GB, Cambridge CB2 1GA, UK

Special Issue Information

Dear Colleagues,

In addition to being the workhorse of the pharmaceutical industry, high-throughput screening is becoming a more and more central experimental approach in diverse research areas ranging from genomics, proteomics to systems and synthetic biology. The idea of characterizing entire ensembles of genes, proteins, small molecule or organismal repertoires is attractive when the complexity of a system defies straightforward understanding and rational manipulation.

In contrast to selection approaches that obey a ‘first-past-the-post’ logic, screening quantitatively measures one or more experimental parameters for the entire library. The knowledge about an entire library may give rise to structure-activity relationships and allows a more informed choice about which molecules to select e.g. for further rounds of evolutionary improvements.

Accessibility of these approaches to a wider circle of experimentalists has been driven by technological advances, specifically miniaturization and advances in detection systems. This issue will focus on the technical advances that give insight into property spaces and combine it with an outlook towards the potential of such massively parallel experimentation in Chemistry and Biology.

Florian Hollfelder, Ph.D.
Guest Editor

Keywords

  • DNA library
  • Encoding /decoding
  • In vitro compartmentalisation
  • Systems biology
  • Synthetic biology

Benefits of Publishing in a Special Issue

  • Ease of navigation: Grouping papers by topic helps scholars navigate broad scope journals more efficiently.
  • Greater discoverability: Special Issues support the reach and impact of scientific research. Articles in Special Issues are more discoverable and cited more frequently.
  • Expansion of research network: Special Issues facilitate connections among authors, fostering scientific collaborations.
  • External promotion: Articles in Special Issues are often promoted through the journal's social media, increasing their visibility.
  • e-Book format: Special Issues with more than 10 articles can be published as dedicated e-books, ensuring wide and rapid dissemination.

Further information on MDPI's Special Issue polices can be found here.

Related Special Issue

Published Papers (8 papers)

Download All Papers
Order results
Result details
Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:

Research

Jump to: Review

199 KiB  
Article
High-Throughput Behavioral Screens: the First Step towards Finding Genes Involved in Vertebrate Brain Function Using Zebrafish
by Robert Gerlai
Molecules 2010, 15(4), 2609-2622; https://doi.org/10.3390/molecules15042609 - 12 Apr 2010
Cited by 175 | Viewed by 13774
Abstract
The zebrafish has been in the forefront of developmental biology for three decades and has become a favorite of geneticists. Due to the accumulated genetic knowledge and tools developed for the zebrafish it is gaining popularity in other disciplines, including neuroscience. The zebrafish [...] Read more.
The zebrafish has been in the forefront of developmental biology for three decades and has become a favorite of geneticists. Due to the accumulated genetic knowledge and tools developed for the zebrafish it is gaining popularity in other disciplines, including neuroscience. The zebrafish offers a compromise between system complexity (it is a vertebrate similar in many ways to our own species) and practical simplicity (it is small, easy to keep, and prolific). Such features make zebrafish an excellent choice for high throughput mutation and drug screening. For the identification of mutation or drug induced alteration of brain function arguably the best methods are behavioral test paradigms. This review does not present experimental examples for the identification of particular genes or drugs. Instead it describes how behavioral screening methods may enable one to find functional alterations in the vertebrate brain. Furthermore, the review is not comprehensive. The behavioral test examples presented are biased according to the personal interests of the author. They will cover research areas including learning and memory, fear and anxiety, and social behavior. Nevertheless, the general principles will apply to other functional domains and should represent a snapshot of the rapidly evolving behavioral screening field with zebrafish. Full article
(This article belongs to the Special Issue High-throughput Screening)
►▼ Show Figures

Figure 1

697 KiB  
Article
HTS-Driven Discovery of New Chemotypes with West Nile Virus Inhibitory Activity
by Dong Hoon Chung, Colleen B. Jonsson, Clinton Maddox, Sara N. McKellip, Blake. P. Moore, Marintha Heil, E. Lucile White, Subramaniam Ananthan, Qianjun Li, Shuang Feng and Lynn Rasmussen
Molecules 2010, 15(3), 1690-1704; https://doi.org/10.3390/molecules15031690 - 12 Mar 2010
Cited by 13 | Viewed by 9763
Abstract
West Nile virus (WNV) is a positive sense, single-stranded RNA virus that can cause illness in humans when transmitted via mosquito vectors. Unfortunately, no antivirals or vaccines are currently available, and therefore efficient and safe antivirals are urgently needed. We developed a high [...] Read more.
West Nile virus (WNV) is a positive sense, single-stranded RNA virus that can cause illness in humans when transmitted via mosquito vectors. Unfortunately, no antivirals or vaccines are currently available, and therefore efficient and safe antivirals are urgently needed. We developed a high throughput screen to discover small molecule probes that inhibit virus infection of Vero E6 cells. A primary screen of a 13,001 compound library at a 10 µM final concentration was conducted using the 384-well format. Z′ values ranged from 0.54–0.83 with a median of 0.74. Average S/B was 17 and S/N for each plate ranged from 10.8 to 23.9. Twenty-six compounds showed a dose response in the HT screen and were further evaluated in a time of addition assay and in a titer reduction assay. Seven compounds showed potential as small molecule probes directed at WNV. The hit rate from the primary screen was 0.185% (24 compounds out of 13,001 compounds) and from the secondary screens was 0.053% (7 out of 13,001 compounds) respectively. Full article
(This article belongs to the Special Issue High-throughput Screening)
►▼ Show Figures

Figure 1

596 KiB  
Article
Hitherto Unrecognized Fluorescence Properties of Coniferyl Alcohol
by Komandoor Elayavalli Achyuthan, Paul David Adams, Supratim Datta, Blake Alexander Simmons and Anup Kumar Singh
Molecules 2010, 15(3), 1645-1667; https://doi.org/10.3390/molecules15031645 - 11 Mar 2010
Cited by 5 | Viewed by 10113
Abstract
We instituted a quasi-quality assurance program for demonstrating coniferyl alcohol’s fluorescence and fluorescence diminishment following enzymatic oxidation. The magnitude of diminishment was a measure of catalysis. High throughput screening was performed in pseudo-kinetic and endpoint modes by measuring the fluorescence at 416 [...] Read more.
We instituted a quasi-quality assurance program for demonstrating coniferyl alcohol’s fluorescence and fluorescence diminishment following enzymatic oxidation. The magnitude of diminishment was a measure of catalysis. High throughput screening was performed in pseudo-kinetic and endpoint modes by measuring the fluorescence at 416 nm following excitation at 290, 310 or 340 nm. Dose-response tracings were linear between two and three orders of magnitude with average limits of detection and quantitation of 1.8 and 6.9 mM coniferyl alcohol, respectively. Oxidation was evident with 0.025 mg/mL laccase or 0.003 mg/mL peroxidase or inside 5 min using 0.5 mg/mL laccase or 5 mM substrate. Sodium chloride inhibited (IC50, 25 mM) laccase oxidation of coniferyl alcohol. Fluorescence from 10 concentrations (1 to 1000 mM) of coniferyl alcohol was stable for 24 hours over 14 excitation/emission cycles at 3 different combinations of excitation and emission wavelengths. In conclusion, coniferyl alcohol absorption and fluorescence assays should facilitate biomass lignin analyses and improve delignification. Full article
(This article belongs to the Special Issue High-throughput Screening)
►▼ Show Figures

Graphical abstract

595 KiB  
Article
Screening and Improvement of an Anti-VEGF DNA Aptamer
by Yoshihiko Nonaka, Koji Sode and Kazunori Ikebukuro
Molecules 2010, 15(1), 215-225; https://doi.org/10.3390/molecules15010215 - 7 Jan 2010
Cited by 126 | Viewed by 14800
Abstract
To obtain an aptamer with a high affinity for vascular endothelial growth factor (VEGF), we focused on the receptor-binding domain (RBD) of VEGF as a target epitope. Three rounds of screening gave Vap7, which bound to the VEGF isoforms VEGF121 and VEGF [...] Read more.
To obtain an aptamer with a high affinity for vascular endothelial growth factor (VEGF), we focused on the receptor-binding domain (RBD) of VEGF as a target epitope. Three rounds of screening gave Vap7, which bound to the VEGF isoforms VEGF121 and VEGF165 with KD values of 1.0 nM and 20 nM, respectively. Moreover, Vap7 showed specificity within the VEGF family. Secondary structure predictions and circular dicrhoism suggested that Vap7 folds into a G-quadruplex structure. We obtained a mutant aptamer that contains only this region of the aptamer sequence. This truncated mutant (V7t1) bound to both VEGF121 and VEGF165 with KD values of 1.1 nM and 1.4 nM, respectively. Its sequence was 5'-TGTGGGGGTGGACGGGCCGGGTAGA-3', and it appeared to form a G-quadruplex structure. We also produced an aptamer heterodimer consisting of our previously derived aptamer (del5-1), which binds to the heparin-binding domain of VEGF, linked to V7t1. The resulting heterodimer bound strongly to VEGF165 with a KD value of 4.7 × 102 pM. Full article
(This article belongs to the Special Issue High-throughput Screening)
►▼ Show Figures

Figure 1

564 KiB  
Article
Spectroscopic Analyses of the Biofuels-Critical Phytochemical Coniferyl Alcohol and Its Enzyme-Catalyzed Oxidation Products
by Komandoor Elayavalli Achyuthan, Paul David Adams, Blake Alexander Simmons and Anup Kumar Singh
Molecules 2009, 14(11), 4758-4778; https://doi.org/10.3390/molecules14114758 - 23 Nov 2009
Cited by 3 | Viewed by 12417
Abstract
Lignin composition (monolignol types of coniferyl, sinapyl or p-coumaryl alcohol) is causally related to biomass recalcitrance. We describe multiwavelength (220, 228, 240, 250, 260, 290, 295, 300, 310 or 320 nm) absorption spectroscopy of coniferyl alcohol and its laccase- or peroxidase-catalyzed products [...] Read more.
Lignin composition (monolignol types of coniferyl, sinapyl or p-coumaryl alcohol) is causally related to biomass recalcitrance. We describe multiwavelength (220, 228, 240, 250, 260, 290, 295, 300, 310 or 320 nm) absorption spectroscopy of coniferyl alcohol and its laccase- or peroxidase-catalyzed products during real time kinetic, pseudokinetic and endpoint analyses, in optical turn on or turn off modes, under acidic or basic conditions. Reactions in microwell plates and 100 μL volumes demonstrated assay miniaturization and high throughput screening capabilities. Bathochromic and hypsochromic shifts along with hyperchromicity or hypochromicity accompanied enzymatic oxidations by laccase or peroxidase. The limits of detection and quantitation of coniferyl alcohol averaged 2.4 and 7.1 μM respectively, with linear trend lines over 3 to 4 orders of magnitude. Coniferyl alcohol oxidation was evident within 10 minutes or with 0.01 μg/mL laccase and 2 minutes or 0.001 μg/mL peroxidase. Detection limit improved to 1.0 μM coniferyl alcohol with Km of 978.7 ± 150.7 μM when examined at 260 nm following 30 minutes oxidation with 1.0 μg/mL laccase. Our assays utilized the intrinsic spectroscopic properties of coniferyl alcohol or its oxidation products for enabling detection, without requiring chemical synthesis or modification of the substrate or product(s). These studies facilitate lignin compositional analyses and augment pretreatment strategies for reducing biomass recalcitrance. Full article
(This article belongs to the Special Issue High-throughput Screening)
►▼ Show Figures

Figure 1

Review

Jump to: Research

846 KiB  
Review
Nanofabrication of Nonfouling Surfaces for Micropatterning of Cell and Microtissue
by Hidenori Otsuka
Molecules 2010, 15(8), 5525-5546; https://doi.org/10.3390/molecules15085525 - 10 Aug 2010
Cited by 29 | Viewed by 12554
Abstract
Surface engineering techniques for cellular micropatterning are emerging as important tools to clarify the effects of the microenvironment on cellular behavior, as cells usually integrate and respond the microscale environment, such as chemical and mechanical properties of the surrounding fluid and extracellular matrix, [...] Read more.
Surface engineering techniques for cellular micropatterning are emerging as important tools to clarify the effects of the microenvironment on cellular behavior, as cells usually integrate and respond the microscale environment, such as chemical and mechanical properties of the surrounding fluid and extracellular matrix, soluble protein factors, small signal molecules, and contacts with neighboring cells. Furthermore, recent progress in cellular micropatterning has contributed to the development of cell-based biosensors for the functional characterization and detection of drugs, pathogens, toxicants, and odorants. In this regards, the ability to control shape and spreading of attached cells and cell-cell contacts through the form and dimension of the cell-adhesive patches with high precision is important. Commitment of stem cells to different specific lineages depends strongly on cell shape, implying that controlled microenvironments through engineered surfaces may not only be a valuable approach towards fundamental cell-biological studies, but also of great importance for the design of cell culture substrates for tissue engineering. To develop this kind of cellular microarray composed of a cell-resistant surface and cell attachment region, micropatterning a protein-repellent surface is important because cellular adhesion and proliferation are regulated by protein adsorption. The focus of this review is on the surface engineering aspects of biologically motivated micropatterning of two-dimensional surfaces with the aim to provide an introductory overview described in the literature. In particular, the importance of non-fouling surface chemistries is discussed. Full article
(This article belongs to the Special Issue High-throughput Screening)
►▼ Show Figures

Graphical abstract

965 KiB  
Review
Assay Format as a Critical Success Factor for Identification of Novel Inhibitor Chemotypes of Tissue-Nonspecific Alkaline Phosphatase from High-Throughput Screening
by Thomas D.Y. Chung, Eduard Sergienko and José Luis Millán
Molecules 2010, 15(5), 3010-3037; https://doi.org/10.3390/molecules15053010 - 27 Apr 2010
Cited by 23 | Viewed by 12134
Abstract
The tissue-nonspecific alkaline phosphatase (TNAP) isozyme is centrally involved in the control of normal skeletal mineralization and pathophysiological abnormalities that lead to disease states such as hypophosphatasia, osteoarthritis, ankylosis and vascular calcification. TNAP acts in concert with the nucleoside triphosphate pyrophosphohydrolase-1 (NPP1) and [...] Read more.
The tissue-nonspecific alkaline phosphatase (TNAP) isozyme is centrally involved in the control of normal skeletal mineralization and pathophysiological abnormalities that lead to disease states such as hypophosphatasia, osteoarthritis, ankylosis and vascular calcification. TNAP acts in concert with the nucleoside triphosphate pyrophosphohydrolase-1 (NPP1) and the Ankylosis protein to regulate the extracellular concentrations of inorganic pyrophosphate (PPi), a potent inhibitor of mineralization. In this review we describe the serial development of two miniaturized high-throughput screens (HTS) for TNAP inhibitors that differ in both signal generation and detection formats, but more critically in the concentrations of a terminal alcohol acceptor used. These assay improvements allowed the rescue of the initially unsuccessful screening campaign against a large small molecule chemical library, but moreover enabled the discovery of several unique classes of molecules with distinct mechanisms of action and selectivity against the related placental (PLAP) and intestinal (IAP) alkaline phosphatase isozymes. This illustrates the underappreciated impact of the underlying fundamental assay configuration on screening success, beyond mere signal generation and detection formats. Full article
(This article belongs to the Special Issue High-throughput Screening)
►▼ Show Figures

Figure 1

279 KiB  
Review
Automation in the High-throughput Selection of Random Combinatorial Libraries—Different Approaches for Select Applications
by Jörn Glökler, Tatjana Schütze and Zoltán Konthur
Molecules 2010, 15(4), 2478-2490; https://doi.org/10.3390/molecules15042478 - 8 Apr 2010
Cited by 24 | Viewed by 9592
Abstract
Automation in combination with high throughput screening methods has revolutionised molecular biology in the last two decades. Today, many combinatorial libraries as well as several systems for automation are available. Depending on scope, budget and time, a different combination of library and experimental [...] Read more.
Automation in combination with high throughput screening methods has revolutionised molecular biology in the last two decades. Today, many combinatorial libraries as well as several systems for automation are available. Depending on scope, budget and time, a different combination of library and experimental handling might be most effective. In this review we will discuss several concepts of combinatorial libraries and provide information as what to expect from these depending on the given context. Full article
(This article belongs to the Special Issue High-throughput Screening)
►▼ Show Figures

Figure 1

Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:
 
Displaying articles 1-8
Back to TopTop