Time-Resolved Luminescence Imaging and Spectroscopy

Dear Colleagues,

Time-resolved luminescence methods are particularly attractive in various biological, biochemical, and biomedical applications, due to their sensitivity and specificity to biochemical, molecular, and physicochemical processes. Being dependent not only on the emitting fluorophore species but also on its molecular environment, time-resolved luminescence allows probing emitting molecules together with their molecular environment, providing both structural and functional information on the investigated specimen. The technological approach, generally based on time-gated or time-correlated single photon counting (TCSPC), has found application in tandem with various optical implementations, including confocal and multiphoton microscopy, macroscopic scanning, and wide-field imaging, and fiber-based spectroscopy and imaging. Time-resolved luminescence methods have been successfully applied to several research topics, ranging from molecular interaction and cell signaling to biological tissue characterization, frequently obtained through functional metabolic imaging of the NAD(P)H-FAD co-enzymes or through phosphorescence of oxygen molecular sensors. This Special Issue aims to review the developments of the technology, publish the latest advances, and illustrate the future prospective of the field.

Dr. Riccardo Cicchi
Guest Editor

Dr. Vladislav Shcheslavskiy
Co-Guest Editor

Dr. João Lagarto
Assistant Guest Editor

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