Legionella and Waterborne Disease

A special issue of Pathogens (ISSN 2076-0817).

Deadline for manuscript submissions: 31 August 2024 | Viewed by 596

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Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, 56126 Pisa, Italy
Interests: healthcare; healthcare quality; health management; hospital management
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Special Issue Information

Dear Colleagues,

Legionella bacteria naturally occur in freshwater environments like lakes and rivers. However, they can also thrive in human-made water systems such as cooling towers, hot tubs, plumbing networks, and decorative fountains. They, primarily Legionella pneumophila, can lead to waterborne diseases when they contaminate water systems and are subsequently inhaled by humans. In Europe, Australia, and the USA, an estimated 10 to 15 cases of Legionellosis per million individuals are officially reported annually. However, in numerous countries, the limited availability of diagnostic tools and surveillance infrastructure leads to an uncertain assessment of its occurrence. The implementation of water management programs to identify Legionella and take steps to minimize the growth and transmission of Legionella are the most critical measures to prevent and control Legionellosis. We invite you to submit original research and review articles related to the issue of Legionella and waterborne diseases. Potential topics include, but are not limited to, the following:

  • Epidemiology: the patterns of Legionellosis occurrence, such as geographic distribution and trends over time.
  • Microbiology: the genetic diversity, growth conditions, antimicrobial resistance, and virulence of the bacteria.
  • Clinical Manifestations: symptoms, disease severity, and the factors that influence whether an infected individual develops Legionnaires' disease or Pontiac fever.
  • Diagnosis: the development of laboratory tests to detect Legionella bacteria or antibodies in patient samples.
  • Prevention: strategies for controlling Legionella contamination in water systems, including the use of disinfection techniques, temperature control, and water management plans.
  • Treatment: effective treatments for Legionellosis, such as studying the susceptibility of different Legionella strains to various antibiotics and identifying factors that may impact treatment outcomes.
  • Public Health Policy: the regulations and guidelines of water safety, outbreak response, public health messaging, environmental monitoring, and policy evaluation.

Dr. Angelo Baggiani
Guest Editor

Manuscript Submission Information

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Keywords

  • Legionella
  • waterborne disease
  • environment
  • Legionnaires' disease
  • Pontiac fever

Published Papers (1 paper)

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Research

15 pages, 2949 KiB  
Article
Effects of Copper on Legionella pneumophila Revealed via Viability Assays and Proteomics
by Yang Song, Didier Mena-Aguilar, Connor L. Brown, William J. Rhoads, Richard F. Helm, Amy Pruden and Marc A. Edwards
Pathogens 2024, 13(7), 563; https://doi.org/10.3390/pathogens13070563 - 3 Jul 2024
Viewed by 454
Abstract
Cu is an antimicrobial that is commonly applied to premise (i.e., building) plumbing systems for Legionella control, but the precise mechanisms of inactivation are not well defined. Here, we applied a suite of viability assays and mass spectrometry-based proteomics to assess the mechanistic [...] Read more.
Cu is an antimicrobial that is commonly applied to premise (i.e., building) plumbing systems for Legionella control, but the precise mechanisms of inactivation are not well defined. Here, we applied a suite of viability assays and mass spectrometry-based proteomics to assess the mechanistic effects of Cu on L. pneumophila. Although a five- to six-log reduction in culturability was observed with 5 mg/L Cu2+ exposure, cell membrane integrity only indicated a <50% reduction. Whole-cell proteomic analysis revealed that AhpD, a protein related to oxidative stress, was elevated in Cu-exposed Legionella relative to culturable cells. Other proteins related to cell membrane synthesis and motility were also higher for the Cu-exposed cells relative to controls without Cu. While the proteins related to primary metabolism decreased for the Cu-exposed cells, no significant differences in the abundance of proteins related to virulence or infectivity were found, which was consistent with the ability of VBNC cells to cause infections. Whereas the cell-membrane integrity assay provided an upper-bound measurement of viability, an amoebae co-culture assay provided a lower-bound limit. The findings have important implications for assessing Legionella risk following its exposure to copper in engineered water systems. Full article
(This article belongs to the Special Issue Legionella and Waterborne Disease)
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