Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
3.0
2.5
Journals
Separations
Special Issues
Natural Plants as Source of Bioactive Compounds: Isolation, Identification and...
share announcement

Natural Plants as Source of Bioactive Compounds: Isolation, Identification and Biological Activity

A special issue of Separations (ISSN 2297-8739). This special issue belongs to the section "Analysis of Natural Products and Pharmaceuticals".

Deadline for manuscript submissions: closed (30 May 2023) | Viewed by 11625

Special Issue Editors

Dr. Raha Orfali

E-Mail Website
Guest Editor
Department of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia
Interests: natural product; antioxidant activity
Dr. Shagufta Perveen

E-Mail Website
Guest Editor
Department of Chemistry, School of Computer, Mathematical and Natural Sciences, Morgan State University, Baltimore, MD 21251, USA
Interests: plant endophytes; LCMS; different biological activities of natural products
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Plant secondary metabolites inspired by these molecules have been initiated since ancient times. It has been found that these metabolites have a broad range of applications, including pharmaceutical, clinical, and even industrial levels. In recent decades, there has been a growing use of advanced methods for extracting, separating, and isolating bioactive natural metabolites. This has emphasized their ability to produce novel compounds that can be directly applied to therapeutic applications, or indirectly used as synthetic or semi-synthetic starting materials for new organic compounds with enhanced properties. Nowadays, various approaches have been developed to discover promising novel bioactive components, such as docking and high-throughput screening. Among others, nano-formulations can improve delivery and pharmacokinetic parameters.

In light of this, the present Special Issue aims to provide an overview of current research on new or known bioactive compounds isolated from plants. We invite researchers with an interest in natural products to submit original or review articles focused on new methods for the isolation, purification, and identification of secondary metabolites. This Special Issue will also include biological evaluation using in vitro, in vivo, and computational studies. In addition, articles on the recent developments of nano-formulations associated with plant-based molecules as a superior delivery and activity enhancer are also encouraged.

Dr. Raha Orfali
Dr. Shagufta Perveen
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Separations is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • plant secondary metabolites
  • phytochemical screening and composition
  • extraction and separation
  • structure elucidation
  • nano-formulation
  • biological activity
  • in vitro study
  • in vivo study
  • docking

Published Papers (4 papers)

Download All Papers
Order results
Result details
Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:

Research

Jump to: Review

20 pages, 4148 KiB  
Article
Identification of Bioactive Compounds in Plant Extracts of Greek Flora and Their Antimicrobial and Antioxidant Activity
by Aliki Tsakni, Archontoula Chatzilazarou, Efstathia Tsakali, Andreas G. Tsantes, Jan Van Impe and Dimitra Houhoula
Separations 2023, 10(7), 373; https://doi.org/10.3390/separations10070373 - 26 Jun 2023
Cited by 4 | Viewed by 3728
Abstract
This study examined five species of the Lamiaceae family, Origanum vulgare L., Origanum dictamnus L., Origanum majorana L., Mentha spicata L. and Lavandula angustifolia L., and one species of the Hypericaceae family, Hypericum perforatum L., for their potential to enhance health and wellbeing [...] Read more.
This study examined five species of the Lamiaceae family, Origanum vulgare L., Origanum dictamnus L., Origanum majorana L., Mentha spicata L. and Lavandula angustifolia L., and one species of the Hypericaceae family, Hypericum perforatum L., for their potential to enhance health and wellbeing or to prevent diseases. An analysis of secondary metabolites was performed by mass spectrometry and HPLC coupled with diode array detection to determine the phenolic compounds included in the plant extracts. The samples were evaluated for their total phenolic content, antioxidant potential, ability to prevent DNA scission caused by peroxyl radicals and antimicrobial activity against pathogens (MIC). All the samples, except Lavandula angustifolia L., had a high DPPH radical scavenging activity and showed significant antibacterial activity. Origanum majorana L. demonstrated the lowest IC50 value (10.31 μg·mL−1). Extracts of medicinal herbs had a remarkably high phenolic content varying between 428 and 1512.74 μg GAE/mg of dry extract, with Origanum vulgare L. having the largest amount of polyphenols. Moreover, the plant extracts exhibited a high level of resistance against DNA damage, with Origanum majorana L. showing the greatest level of protection with 98.05% inhibition. According to the results of this study, these plants from the Greek flora provide beneficial effects for health as natural antioxidants. A baseline can be established through the analysis of the findings of this survey for future research on the same plant species. Full article
(This article belongs to the Special Issue Natural Plants as Source of Bioactive Compounds: Isolation, Identification and Biological Activity)
►▼ Show Figures

Figure 1

14 pages, 868 KiB  
Article
Swietenia mahagoni Leaves Extract: Antifungal, Insecticidal, and Phytochemical Analysis
by Wael M. Khamis, Ahmed A. Heflish, Sarah El-Messeiry, Said I. Behiry, Abdulaziz A. Al-Askar, Yiming Su, Ahmed Abdelkhalek and Mohamed K. Gaber
Separations 2023, 10(5), 301; https://doi.org/10.3390/separations10050301 - 9 May 2023
Cited by 6 | Viewed by 2528
Abstract
In this study, we investigated the antifungal properties of an acetone extract derived from the leaves of Swietenia mahagoni (SMAL) against two isolated fungi, Fusarium equiseti (OQ820153) and Rhizoctonia solani (OQ820152), from rice sheath. The extract was effective in inhibiting the growth of [...] Read more.
In this study, we investigated the antifungal properties of an acetone extract derived from the leaves of Swietenia mahagoni (SMAL) against two isolated fungi, Fusarium equiseti (OQ820153) and Rhizoctonia solani (OQ820152), from rice sheath. The extract was effective in inhibiting the growth of both fungi at the highest concentration tested, 3000 μg·mL−1. Laboratory tests on the LC20 of SMAL extract (49.86 mg·L−1) versus pyriproxyfen 10% EC (1.96 mg·L−1) were accomplished on Aphis gossypii Glover. The extract potently reduced the survival of the nymphs (49.58%) more than the other treatments. The longevity of nymphs treated with the extract had the highest prolongation at 9.67 days. The olfactory choice test exhibited the lowest aphid attraction percentage (23.33%). The HPLC of SMAL extract contained various phenolic compounds, and the most abundant found were catechin (752.64 µg·g−1), gallic acid, and chlorogenic acid, as well as flavonoids such as rutin (585.24 µg·g−1) and naringenin. A GC–MS analysis revealed n-hexadecanoic acid (37.1%) as the major compound, followed by oleic acid. These results suggest that SMAL extract has the potential to help plants fight against fungal and insect infections, making it a promising natural and renewable solution for long-term plant pest regulation. Full article
(This article belongs to the Special Issue Natural Plants as Source of Bioactive Compounds: Isolation, Identification and Biological Activity)
►▼ Show Figures

Graphical abstract

16 pages, 5901 KiB  
Article
Lipids Fraction from Caralluma europaea (Guss.): MicroTOF and HPLC Analyses and Exploration of Its Antioxidant, Cytotoxic, Anti-Inflammatory, and Wound Healing Effects
by Fatima Ez-Zahra Amrati, Meryem Slighoua, Ibrahim Mssillou, Mohamed Chebaibi, Renata Galvão de Azevedo, Smahane Boukhira, Karina Moslova, Omkulthom Al Kamaly, Asmaa Saleh, André Correa de Oliveira, Alice de Freitas Gomes, Gemilson Soares Pontes and Dalila Bousta
Separations 2023, 10(3), 172; https://doi.org/10.3390/separations10030172 - 3 Mar 2023
Cited by 4 | Viewed by 2036
Abstract
Caralluma europaea is a medicinal plant used in Morocco to cure a variety of illnesses. This study was conducted to determine the chemical composition, the antioxidant, antiproliferative, anti-inflammatory, and wound healing activities of C. europaea lipids. The chemical composition of C. europaea was [...] Read more.
Caralluma europaea is a medicinal plant used in Morocco to cure a variety of illnesses. This study was conducted to determine the chemical composition, the antioxidant, antiproliferative, anti-inflammatory, and wound healing activities of C. europaea lipids. The chemical composition of C. europaea was analyzed using time-of-flight mass spectrometry and high-performance liquid chromatography. The antioxidant potential was determined using the 2,2-di-phenyl-1-picryl hydrazyl (DPPH), and ferric reducing antioxidant power (FRAP) tests. The antiproliferative effect was evaluated by MTT assay against HL60, K562, Huh-7 cancer cells, and normal Vero cells. The anti-inflammatory potential was conducted against carrageenan-induced paw edema. The wound healing effect was evaluated against skin burns for 21 days. The identified phytochemical compounds were docked for their effect on nicotinamide adenine dinucleotide phosphate oxidase, caspase-3, lipoxygenase, glycogen synthase kinase-3-β, and protein casein kinase-1. The results showed the presence of some lipids, such as linoleic acid and vitamin D3. The DPPH (IC50 = 0.018 mg/mL) and FRAP (EC50 = 0.084 mg/mL) of C. europaea lipids showed an important antioxidant effect. For the anti-inflammatory test, an inhibition of 83.50% was recorded after 6 h of treatment. Our extract showed the greatest wound retraction on the 21st day (98.20%). C. europaea lipids showed a remarkable antitumoral effect against the K562 cell line (IC50 = 37.30 µg/mL), with no effect on Vero cells (IC50 > 100 µg/mL). Lignoceric acid was the most active molecule against caspase-3 (−6.453 kcal/mol). The findings indicate the growing evidence of C. europaea as a potential treatment for several diseases. Full article
(This article belongs to the Special Issue Natural Plants as Source of Bioactive Compounds: Isolation, Identification and Biological Activity)
►▼ Show Figures

Graphical abstract

Review

Jump to: Research

19 pages, 2629 KiB  
Review
Traditional Importance, Phytochemistry, Pharmacology, and Toxicological Attributes of the Promising Medicinal Herb Carissa spinarum L.
by Nitin Sharma, Vikas Kumar, Nidhi Gupta, Pratyush Shekhar and Palki Sahib Kaur
Separations 2023, 10(3), 158; https://doi.org/10.3390/separations10030158 - 24 Feb 2023
Cited by 1 | Viewed by 2831
Abstract
Carissa spinarum L. (Apocynaceae), commonly known as Garna or Jungli Karonda, has a rich history of use in indigenous traditional medicinal systems owing to its tremendous medicinal and nutritional benefits. The present review aims to discuss the traditional uses, ethnopharmacology, bioactive composition, toxicity [...] Read more.
Carissa spinarum L. (Apocynaceae), commonly known as Garna or Jungli Karonda, has a rich history of use in indigenous traditional medicinal systems owing to its tremendous medicinal and nutritional benefits. The present review aims to discuss the traditional uses, ethnopharmacology, bioactive composition, toxicity analysis, and biotechnological applications of Carissa spinarum L. (CS) to identify the gap between current applications and research conducted on this plant. We collected the literature published before December 2022 on the phytochemical composition, pharmacological properties, and biotechnological applications of CS. Literature in English from scientific databases such as Google Scholar, PubMed, ScienceDirect, Springer, and Wiley, along with books on CS, was analyzed and summarized to prepare this review. The plant taxonomy was verified using the “World Flora Online” database. The in vitro and in vivo pharmacological studies on CS revealed its anthelmintic, anticonvulsant, anti-arthritic, anti-inflammatory, antimicrobial, antioxidant, antidiabetic, hepatoprotective, vasorelaxant, antihypertensive, antitumor, wound-healing, anti-venom, and antipyretic effects. Toxicological studies on CS also indicated the absence of any adverse effects even at high doses after oral administration. Although CS showed remarkable therapeutic activities against several diseases—such as diabetes, cancer, inflammation, and hepatitis B virus—there are several drawbacks in previous reports, including the lack of information on the drug dose, standards, controls, and mechanism of action of the extract or the phytocompounds responsible for its activity. Extensive research with proper in vivo or in vitro model systems is required to validate its reported activities. Full article
(This article belongs to the Special Issue Natural Plants as Source of Bioactive Compounds: Isolation, Identification and Biological Activity)
►▼ Show Figures

Graphical abstract

Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:
 
Displaying articles 1-4
Back to TopTop