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Separations
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Advances in Hyphenated Methods in Separation
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Advances in Hyphenated Methods in Separation

A special issue of Separations (ISSN 2297-8739).

Deadline for manuscript submissions: closed (30 September 2014) | Viewed by 24713

Special Issue Editor

Prof. Dr. Gianni Galaverna

E-Mail Website
Guest Editor
Department of Food and Drug, University of Parma, Parco Area delle Scienze, 17/a 43124 Parma, Italy
Interests: chromatography, mass spectrometry, metabolomics, food analysis, food safety, mycotoxins
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Hyphenated separation methods have advanced significantly in the past decade and new solutions and improvements are continuously being developed, enormously expanding the possibilities of analytical science. As the complexity of a sample is frequently much higher than one could have imagined in the past, fully exploiting the potential of separation (chromatography, electrophoresis, etc.) and detection system (mass spectrometry, NMR, etc.) with a multidimensional approach has really unlocked the door to a more efficient investigation and structural elucidation of analytes in different fields, i.e. the study of biological processes, food science, environmental science, biomedical and pharmaceutical science, etc. Particularly, so called hyphenated dimensions, combining several techniques both in separation (multidimensional techniques) and detection (mass spectrometry, NMR, multiple detectors), are currently offering a powerful methodology for all the -omic sciences, in which targeting both the knowns and the unknowns is a fundamental requisite. An efficient management of the enormous amount of data currently extractable by a complex sample is another challenge in this field. Therefore, this special issue will be devoted to new developments, new technological achievements, as well as novel applications of hyphenated methods in separation sciences as key instruments to unravel the complexity.

Dr. Gianni Galaverna
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Separations is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.


Keywords

  • hyphenated techniques
  • separation
  • mass spectrometry
  • gas chromatography
  • liquid chromatography

Published Papers (3 papers)

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3327 KiB  
Article
Multivariate DoE Optimization of Asymmetric Flow Field Flow Fractionation Coupled to Quantitative LC-MS/MS for Analysis of Lipoprotein Subclasses
by Zsuzsanna Kuklenyik, Michael S. Gardner, Bryan A. Parks, David M. Schieltz, Jon C. Rees, Lisa G. McWilliams, Yulanda M. Williamson, James L. Pirkle and John R. Barr
Chromatography 2015, 2(1), 96-117; https://doi.org/10.3390/chromatography2010096 - 26 Feb 2015
Cited by 11 | Viewed by 8315
Abstract
In this report we demonstrate a practical multivariate design of experiment (DoE) approach for asymmetric flow field-flow fractionation (AF4) method optimization using separation of lipoprotein subclasses as an example. First, with the aid of commercially available software, we built a full factorial screening [...] Read more.
In this report we demonstrate a practical multivariate design of experiment (DoE) approach for asymmetric flow field-flow fractionation (AF4) method optimization using separation of lipoprotein subclasses as an example. First, with the aid of commercially available software, we built a full factorial screening design where the theoretical outcomes were calculated by applying established formulas that govern AF4 channel performance for a 5–35 nm particle size range of interest for lipid particles. Second, using the desirable ranges of instrumental parameters established from theoretical optimization, we performed fractional factorial DoE for AF4 separation of pure albumin and ferritin with UV detection to narrow the range of instrumental parameters and allow optimum size resolution while minimizing losses from membrane immobilization. Third, the optimal range of conditions were tested using response surface DoE for sub-fractionation of high and low density lipoproteins (HDL and LDL) in human serum, where the recovery of the analytes were monitored by fraction collection and isotope-dilution LC-MS/MS analysis of each individual fraction for cholesterol and apolipoproteins (ApoA-1 and ApoB-100). Our results show that DoE is an effective tool in combining AF4 theoretical knowledge and experimental data in finding the most optimal set of AF4 instrumental parameters for quantitative coupling with LC-MS/MS measurements. Full article
(This article belongs to the Special Issue Advances in Hyphenated Methods in Separation)
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1228 KiB  
Article
Analyses of Phytohormones in Coconut (Cocos Nucifera L.) Water Using Capillary Electrophoresis-Tandem Mass Spectrometry
by Swee Ngin Tan, Jean Wan Hong Yong and Liya Ge
Chromatography 2014, 1(4), 211-226; https://doi.org/10.3390/chromatography1040211 - 22 Dec 2014
Cited by 17 | Viewed by 10111
Abstract
Capillary electrophoresis (CE) coupled with mass spectrometry (MS) or tandem mass spectrometry (MS/MS) is reported as an alternative and potentially useful method for the simultaneous analysis of various classes of phytohormones with diversified structures, including indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), abscisic acid [...] Read more.
Capillary electrophoresis (CE) coupled with mass spectrometry (MS) or tandem mass spectrometry (MS/MS) is reported as an alternative and potentially useful method for the simultaneous analysis of various classes of phytohormones with diversified structures, including indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), abscisic acid (ABA), gibberellic acid (GA), zeatin (Z), N6-benzyladenine (BA), α-naphthaleneacetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-D). The key to the CE-MS/MS analysis was based on electroosmotic flow reversal using a cationic polymer-coated capillary. Under optimum conditions, a baseline separation of eight phytohormones was accomplished within 30 min using 60 mM ammonium formate/formic acid buffer of pH 3.8 with −20 kV as the separation voltage. The accessibility of MS/MS together with the characterization by migration properties obtained by CE allows for the development of CE-MS/MS as an emerging potential method for the analysis of different classes of phytohormones in a single run. The utility of the CE-MS/MS method was demonstrated by the comprehensive screening of phytohormones in coconut (Cocos nucifera L.) water after pre-concentration and purification through solid-phase extraction (SPE) cartridge. IAA, ABA, GA and Z were detected and quantified in the purified coconut water extract sample. Full article
(This article belongs to the Special Issue Advances in Hyphenated Methods in Separation)
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257 KiB  
Article
Cu Purification Using an Extraction Resin for Determination of Isotope Ratios by Multicollector ICP-MS
by Akio Makishima
Chromatography 2014, 1(3), 96-107; https://doi.org/10.3390/chromatography1030096 - 25 Jun 2014
Cited by 1 | Viewed by 5799
Abstract
A new simple and quick method has been established for separation of Cu from solutions using an extraction chromatographic resin utilizing Aliquat® 336 (commercially available as TEVA™ resin) and Cu(I). This method involves the use of a one milliliter column containing 0.33 [...] Read more.
A new simple and quick method has been established for separation of Cu from solutions using an extraction chromatographic resin utilizing Aliquat® 336 (commercially available as TEVA™ resin) and Cu(I). This method involves the use of a one milliliter column containing 0.33 mL TEVA™ resin on 0.67 mL Amberchrom® CG-71C acrylic resin. Copper was adsorbed on the column by forming Cu(I) with 0.15% ascorbic acid in 0.05 mol·L−1 HBr, while other major elements except Zn showed no adsorption. After removal of the major elements (Na, Mg, Al, P, K, Ca, Cr, Mn, Fe, Co and Ni), Cu was recovered using 2 mol·L−1 HNO3. The recovery yield and total blank were 102% ± 2% and 0.25 ng, respectively. To evaluate the separation method, Cu isotope ratios were determined by a standard-sample-standard bracketing method using multicollector inductively coupled plasma-mass spectrometry (ICP-MS), with a repeatability of 0.04‰ and 0.25‰ (SD), for the standard solution and the solutions from low S (<0.1% S) silicate standards, respectively. Full article
(This article belongs to the Special Issue Advances in Hyphenated Methods in Separation)
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