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Separations
Special Issues
Application of Chromatography in Biomolecular Chemistry
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Application of Chromatography in Biomolecular Chemistry

A special issue of Separations (ISSN 2297-8739).

Deadline for manuscript submissions: closed (31 March 2021) | Viewed by 2217

Special Issue Editor

Dr. Ryuichi Mashima

E-Mail Website
Guest Editor
National Center for Child Health and Development, Tokyo, Japan
Interests: liquid chromatography; gas chromatography; clinical laboratory medicine; biochemistry; food science; pharmaceutical science; solid phase extraction; mass spectrometry; derivatization

Special Issue Information

Dear Colleagues,

I am happy to inform you that the Special Issue: “Application of Chromatography in Biomolecular Chemistry” is currently being prepared by the journal Separations.

Research papers targeting bimolecular chemistry, which involve clinical laboratory medicine, related biochemistry, food science, and pharmaceutical science were highly invited to this issue. Technical papers regarding assay validation, mass spectrometry, and derivatization techniques, as well as sample preparation, including solid phase extraction (SPE), are also welcome.

Liquid chromatography and gas chromatography are the relevant chromatographic techniques in this area. Furthermore, short notes regarding superficial chromatography (SFC) and thin-layer chromatography with mass spectrometric detection are also within our scope.

Dr. Ryuichi Mashima
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Separations is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • liquid chromatography
  • gas chromatography
  • clinical laboratory medicine
  • biochemistry
  • food science
  • pharmaceutical science
  • solid phase extraction
  • mass spectrometry
  • derivatization

Published Papers (1 paper)

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Research

11 pages, 7176 KiB  
Article
Long-Chain Base (LCB)-Targeted Lipidomics Study Uncovering the Presence of a Variety of LCBs in Mammalian Blood
by Mari Ohira, Torayuki Okuyama and Ryuichi Mashima
Separations 2020, 7(4), 57; https://doi.org/10.3390/separations7040057 - 23 Oct 2020
Viewed by 2819
Abstract
Globotriaosylsphingosine (LysoGb3) is a biomarker for Fabry disease (OMIM 301500) that contains long-chain bases (LCBs) as a building block. There have been several studies proposing that LysoGb3 forms with distinct LCBs could be putative disease subtype-related biomarkers for this congenital [...] Read more.
Globotriaosylsphingosine (LysoGb3) is a biomarker for Fabry disease (OMIM 301500) that contains long-chain bases (LCBs) as a building block. There have been several studies proposing that LysoGb3 forms with distinct LCBs could be putative disease subtype-related biomarkers for this congenital disorder; however, there have been no detailed multiple reaction monitoring-based studies examining the LCB distribution in this lysosphingolipid. To achieve this, we established an assay procedure that aimed at elucidating the LCB-targeted lipidome using liquid chromatography–tandem mass spectrometry. Consistent with previous studies, we found d18:1 to be the major LCB species of the LysoGb3 in pooled human plasma, while some atypical LCBs, such as d18:2, d18:0, t18:1, d16:1, and d17:1, were detected as minor fractions. When the same methodology was applied to fetal bovine serum (FBS) as a positive control, we identified additional unique LCB species, such as t18:0, d20:1, t19:1, and t21:1, in herbivore LysoGb3. Furthermore, we found an elevation of sphingosine and LysoGb3, which are N-deacylated forms of ceramide and Gb3, respectively, in FBS, suggesting that ceramidase activity may be involved in this process. Thus, our LCB-targeted lipidomics data revealed that mammalian LCBs in glycosphingolipids have a greater variety of molecular species than previously expected. Full article
(This article belongs to the Special Issue Application of Chromatography in Biomolecular Chemistry)
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