Molecular Diagnostic Methods in Veterinary Pathology Investigation of Viral Diseases

A special issue of Veterinary Sciences (ISSN 2306-7381). This special issue belongs to the section "Veterinary Microbiology, Parasitology and Immunology".

Deadline for manuscript submissions: closed (21 May 2023) | Viewed by 2785

Special Issue Editor


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Guest Editor
Department of Animal Medicine, Production and Health, Università degli Studi di Padova, Viale dell’Università 16, 35020 Legnaro, PD, Italy
Interests: veterinary epidemiology; virus; molecular biology; phylogenetics; phylodynamics; poultry diseases; swine diseases; wildlife diseases

Special Issue Information

Dear Colleagues,

There is universal consensus regarding the importance of molecular methods for the investigation of animal viruses, both within the diagnostic routine and for research purposes. The progress made in the field of molecular diagnostics over the years, along with their ever-increasing accessibility, led to a better understanding of the pathobiology, epizootiology and evolution of viral pathogens affecting companion animals, livestock and wildlife, some of which possess zoonotic relevance. In turn, the generated data have proved beneficial to enhance their control, informing and enhancing the prophylactic and therapeutic measures adopted against many burdensome diseases. In addition, the application of next-generation sequencing allowed for the discovery and characterization of an unprecedented number of new viral species in a diverse range of animal hosts.

The aim of this Special Issue is to collect notable experiences showing the benefits of applying molecular assays to study viral pathogens of established or potential veterinary relevance. Any original research on the validation of novel molecular techniques or improving ones which are already in use is also welcomed, as are reviews based on available molecular surveys and sequencing data.

Dr. Matteo Legnardi
Guest Editor

Manuscript Submission Information

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Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • virus
  • viral diseases
  • pathology
  • epidemiology
  • molecular biology
  • assay validation
  • next generation sequencing
  • domestic animals
  • wildlife

Published Papers (1 paper)

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Research

16 pages, 1985 KiB  
Article
Genetic Diversity of Newcastle Disease Virus Involved in the 2021 Outbreaks in Backyard Poultry Farms in Tanzania
by Charlie F. Amoia, Jean N. Hakizimana, Nisha K. Duggal, Augustino A. Chengula, Mohammed A. Rohaim, Muhammad Munir, James Weger-Lucarelli and Gerald Misinzo
Vet. Sci. 2023, 10(7), 477; https://doi.org/10.3390/vetsci10070477 - 21 Jul 2023
Cited by 1 | Viewed by 2172
Abstract
Newcastle disease virus is a significant avian pathogen with the potential to decimate poultry populations all over the world and cause enormous economic losses. Distinct NDV genotypes are currently causing outbreaks worldwide. Due to the high genetic diversity of NDV, virulent strains that [...] Read more.
Newcastle disease virus is a significant avian pathogen with the potential to decimate poultry populations all over the world and cause enormous economic losses. Distinct NDV genotypes are currently causing outbreaks worldwide. Due to the high genetic diversity of NDV, virulent strains that may result in a lack of vaccine protection are more likely to emerge and ultimately cause larger epidemics with massive economic losses. Thus, a more comprehensive understanding of the circulating NDV genotypes is critical to reduce Newcastle disease (ND) burden. In this study, NDV strains were isolated and characterized from backyard poultry farms from Tanzania, East Africa in 2021. Reverse-transcription polymerase chain reaction (RT-PCR) based on fusion (F) gene amplification was conducted on 79 cloacal or tracheal swabs collected from chickens during a suspected ND outbreak. Our results revealed that 50 samples out 79 (50/79; 63.3%) were NDV-positive. Sequencing and phylogenetic analyses of the selected NDV isolates showed that 39 isolates belonged to subgenotype VII.2 and only one isolate belonged to subgenotype XIII.1.1. Nucleotide sequences of the NDV F genes from Tanzania were closely related to recent NDV isolates circulating in southern Africa, suggesting that subgenotype VII.2 is the predominant subgenotype throughout Tanzania and southern Africa. Our data confirm the circulation of two NDV subgenotypes in Tanzania, providing important information to design genotype-matched vaccines and to aid ND surveillance. Furthermore, these results highlight the possibility of the spread and emergence of new NDV subgenotypes with the potential of causing future ND epizootics. Full article
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