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Flow Virometry: A New Tool for Studying Viruses

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Special Issue Editor

Dr. John Yin

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Guest Editor

Department of Chemical and Biological Engineering, University of Wisconsin-Madison, 1415 Engineering Drive, Madison, WI 53706, USA
Interests: virus–host interactions; systems biology; quantitative virology; computational biology; origins of life; systems chemistry; prebiotic chemistry

Special Issue Information

Dear Colleagues,

We cordially invite you to contribute your groundbreaking research in the field of Flow Virometry to our upcoming issue of Viruses. As the Editor, I am excited to showcase your innovative work characterizing virus and virus-like particles at the frontiers of quantitative virology.

Flow Virometry promises to open new opportunities in the high-throughput physical, chemical, and biological characterization of viruses and virus-like particles, with impacts on diagnostics, viral pathogenesis, therapeutics, and vaccines. We welcome your manuscripts and offer you a platform to share your discoveries and insights with a broad audience. Your contributions will drive this emerging field forward and lead to a better understanding of viruses as readily quantifiable physical and chemical nanoparticles with encoded biology.

Submit your manuscripts by April 2024, and let us shape the future of virology together. Join us in this exciting journey of discovery.

Dr. John Yin
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Viruses is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

flow virometry
virus particle detection
virus counting
virus labeling/staining
virus-like particles
scanning ion occlusion sensing

Published Papers (2 papers)

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Research

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20 pages, 11056 KiB

Open AccessArticle

Premature Activation of the HIV-1 Protease Is Influenced by Polymorphisms in the Hinge Region

by Caroline O. Tabler, Sarah J. Wegman, Najwa Alhusaini, Nicole F. Lee and John C. Tilton

Viruses 2024, 16(6), 849; https://doi.org/10.3390/v16060849 (registering DOI) - 26 May 2024

Viewed by 98

Abstract

HIV-1 protease inhibitors are an essential component of antiretroviral therapy. However, drug resistance is a pervasive issue motivating a persistent search for novel therapies. Recent reports found that when protease activates within the host cell’s cytosol, it facilitates the pyroptotic killing of infected cells. This has led to speculation that promoting protease activation, rather than inhibiting it, could help to eradicate infected cells and potentially cure HIV-1 infection. Here, we used a nanoscale flow cytometry-based assay to characterize protease resistance mutations and polymorphisms. We quantified protease activity, viral concentration, and premature protease activation and confirmed previous findings that major resistance mutations generally destabilize the protease structure. Intriguingly, we found evidence that common polymorphisms in the hinge domain of protease can influence its susceptibility to premature activation. This suggests that viral heterogeneity could pose a considerable challenge for therapeutic strategies aimed at inducing premature protease activation in the future. Full article

(This article belongs to the Special Issue Flow Virometry: A New Tool for Studying Viruses)

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Review

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11 pages, 967 KiB

Open AccessReview

Analysis of Individual Viral Particles by Flow Virometry

by Caroline O. Tabler and John C. Tilton

Viruses 2024, 16(5), 802; https://doi.org/10.3390/v16050802 - 18 May 2024

Viewed by 356

Abstract

This review focuses on the emerging field of flow virometry, the study and characterization of individual viral particles using flow cytometry instruments and protocols optimized for the detection of nanoscale events. Flow virometry faces considerable technical challenges including minimal light scattering by small viruses that complicates detection, coincidental detection of multiple small particles due to their high concentrations, and challenges with sample preparation including the inability to easily “wash” samples to remove unbound fluorescent antibodies. We will discuss how the field has overcome these challenges to reveal novel insights into viral biology. Full article

(This article belongs to the Special Issue Flow Virometry: A New Tool for Studying Viruses)

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