Application of Genetically Engineered Plant Viruses

A special issue of Viruses (ISSN 1999-4915). This special issue belongs to the section "Viruses of Plants, Fungi and Protozoa".

Deadline for manuscript submissions: 28 February 2025 | Viewed by 935

Special Issue Editor


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Guest Editor
Department of Medical Laboratory Science and Biotechnology, Asia University, Taichung City, Taiwan
Interests: plant virology; plant pathology

Special Issue Information

Dear Colleagues,

Plant viruses seriously affect crop cultivation and agricultural production worldwide. They are not always a threat but can be friends. Infectious clones of many plant viruses have been constructed and modified through genetic engineering for the study of viral pathogenesis. Attenuated plant viruses can be used as vaccines for crop protection. Viral vectors engineered from infectious clones of plant viruses are effective tools for overexpressing genes of interest in plants for human/animal disease treatment and health. Plant viral vectors have also been exploited to knock down plant gene expression through virus-induced gene silencing.

In this Special Issue, we welcome a wide range of articles, including original research, short communications, and reviews, that focus on the application of genetically engineered plant viruses in agriculture, medicine, human/animal health, and other fields. Your research achievements will significantly contribute to enhancing our understanding of this field. We look forward to receiving your submissions for this Special Issue.

Prof. Dr. Tsung-Chi Chen
Guest Editor

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Keywords

  • plant virus
  • infectious clone
  • viral vector
  • virus-induced gene silencing (VIGS)
  • virus-like particles (VLPs)
  • cross-protection

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Published Papers (1 paper)

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Research

15 pages, 3799 KiB  
Article
The Replicase Protein of Potato Virus X Is Able to Recognize and Trans-Replicate Its RNA Component
by Pinky Dutta, Andres Lõhmus, Tero Ahola and Kristiina Mäkinen
Viruses 2024, 16(10), 1611; https://doi.org/10.3390/v16101611 - 15 Oct 2024
Viewed by 731
Abstract
The trans-replication system explores the concept of separating the viral RNA involved in the translation of the replicase protein from the replication of the viral genome and has been successfully used to study the replication mechanisms of alphaviruses. We tested the feasibility [...] Read more.
The trans-replication system explores the concept of separating the viral RNA involved in the translation of the replicase protein from the replication of the viral genome and has been successfully used to study the replication mechanisms of alphaviruses. We tested the feasibility of this system with potato virus X (PVX), an alpha-like virus, in planta. A viral RNA template was designed which does not produce the replicase and prevents virion formation but remains recognizable by the replicase. The replicase construct encodes for the replicase protein, while lacking other virus-specific recognition sequences. Both the constructs were delivered into Nicotiana benthamiana leaves via Agrobacterium-mediated infiltration. Templates of various lengths were tested, with the longer templates not replicating at 4 and 6 days post inoculation, when the replicase protein was provided in trans. Co-expression of helper component proteinase with the short template led to its trans-replication. The cells where replication had been initiated were observed to be scattered across the leaf lamina. This study established that PVX is capable of trans-replicating and can likely be further optimized, and that the experimental freedom offered by the system can be utilized to delve deeper into understanding the replication mechanism of the virus. Full article
(This article belongs to the Special Issue Application of Genetically Engineered Plant Viruses)
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