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Viruses
Special Issues
Application of Genetically Engineered Plant Viruses
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Application of Genetically Engineered Plant Viruses

A special issue of Viruses (ISSN 1999-4915). This special issue belongs to the section "Viruses of Plants, Fungi and Protozoa".

Deadline for manuscript submissions: 28 February 2025 | Viewed by 1802

Special Issue Editor

Prof. Dr. Tsung-Chi Chen

E-Mail Website
Guest Editor
Department of Medical Laboratory Science and Biotechnology, Asia University, Taichung City, Taiwan
Interests: plant virology; plant pathology

Special Issue Information

Dear Colleagues,

Plant viruses seriously affect crop cultivation and agricultural production worldwide. They are not always a threat but can be friends. Infectious clones of many plant viruses have been constructed and modified through genetic engineering for the study of viral pathogenesis. Attenuated plant viruses can be used as vaccines for crop protection. Viral vectors engineered from infectious clones of plant viruses are effective tools for overexpressing genes of interest in plants for human/animal disease treatment and health. Plant viral vectors have also been exploited to knock down plant gene expression through virus-induced gene silencing.

In this Special Issue, we welcome a wide range of articles, including original research, short communications, and reviews, that focus on the application of genetically engineered plant viruses in agriculture, medicine, human/animal health, and other fields. Your research achievements will significantly contribute to enhancing our understanding of this field. We look forward to receiving your submissions for this Special Issue.

Prof. Dr. Tsung-Chi Chen
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Viruses is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • plant virus
  • infectious clone
  • viral vector
  • virus-induced gene silencing (VIGS)
  • virus-like particles (VLPs)
  • cross-protection

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Published Papers (2 papers)

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Research

21 pages, 6259 KiB  
Article
Rapid and Visual Screening of Virus Infection in Sugar Beets Through Polerovirus-Induced Gene Silencing
by Heemee Devi Bunwaree, Elodie Klein, Guillaume Saubeau, Bruno Desprez, Véronique Ziegler-Graff and David Gilmer
Viruses 2024, 16(12), 1823; https://doi.org/10.3390/v16121823 - 23 Nov 2024
Viewed by 595
Abstract
Since the ban of neonicotinoid insecticides in the European Union, sugar beet production is threatened by outbreaks of virus yellows (VY) disease, caused by several aphid-transmitted viruses, including the polerovirus beet mild yellowing virus (BMYV). As the symptoms induced may vary depending on [...] Read more.
Since the ban of neonicotinoid insecticides in the European Union, sugar beet production is threatened by outbreaks of virus yellows (VY) disease, caused by several aphid-transmitted viruses, including the polerovirus beet mild yellowing virus (BMYV). As the symptoms induced may vary depending on multiple infections and other stresses, there is an urgent need for fast screening tests to evaluate resistance/tolerance traits in sugar beet accessions. To address this issue, we exploited the virus-induced gene silencing (VIGS) system, by introducing a fragment of a Beta vulgaris gene involved in chlorophyll synthesis in the BMYV genome. This recombinant virus was able to generate early clear vein chlorosis symptoms in infected sugar beets, allowing easy and rapid visual discernment of infected plants across five sugar beet lines. The recombinant virus displayed similar infectivity as the wild-type, and the insert remained stable within the viral progeny. We demonstrated that the percentage of VIGS-symptomatic plants was representative of the infection rate of each evaluated line, and depending on the susceptibility of the line to BMYV infection, VIGS symptoms may last over months. Our work provides a polerovirus-based VIGS system adapted to sugar beet crop allowing visual and rapid large-scale screens for resistance or functional genomic studies. Full article
(This article belongs to the Special Issue Application of Genetically Engineered Plant Viruses)
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15 pages, 3799 KiB  
Article
The Replicase Protein of Potato Virus X Is Able to Recognize and Trans-Replicate Its RNA Component
by Pinky Dutta, Andres Lõhmus, Tero Ahola and Kristiina Mäkinen
Viruses 2024, 16(10), 1611; https://doi.org/10.3390/v16101611 - 15 Oct 2024
Viewed by 916
Abstract
The trans-replication system explores the concept of separating the viral RNA involved in the translation of the replicase protein from the replication of the viral genome and has been successfully used to study the replication mechanisms of alphaviruses. We tested the feasibility [...] Read more.
The trans-replication system explores the concept of separating the viral RNA involved in the translation of the replicase protein from the replication of the viral genome and has been successfully used to study the replication mechanisms of alphaviruses. We tested the feasibility of this system with potato virus X (PVX), an alpha-like virus, in planta. A viral RNA template was designed which does not produce the replicase and prevents virion formation but remains recognizable by the replicase. The replicase construct encodes for the replicase protein, while lacking other virus-specific recognition sequences. Both the constructs were delivered into Nicotiana benthamiana leaves via Agrobacterium-mediated infiltration. Templates of various lengths were tested, with the longer templates not replicating at 4 and 6 days post inoculation, when the replicase protein was provided in trans. Co-expression of helper component proteinase with the short template led to its trans-replication. The cells where replication had been initiated were observed to be scattered across the leaf lamina. This study established that PVX is capable of trans-replicating and can likely be further optimized, and that the experimental freedom offered by the system can be utilized to delve deeper into understanding the replication mechanism of the virus. Full article
(This article belongs to the Special Issue Application of Genetically Engineered Plant Viruses)
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