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Viruses
Special Issues
Organoid-Based Virus Research
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Organoid-Based Virus Research

A special issue of Viruses (ISSN 1999-4915). This special issue belongs to the section "Human Virology and Viral Diseases".

Deadline for manuscript submissions: closed (29 February 2024) | Viewed by 4896

Special Issue Editors

Dr. Jie Zhou

E-Mail Website
Guest Editor
Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China
Interests: adult stem cell-derived organoids; virus-host interaction of respiratory viruses; viral pathogenesis
Dr. Hin Chu

E-Mail Website
Guest Editor
State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, The University of Hong Kong, Hong Kong
Interests: viral pathogenesis; virus-host interactions
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

We are delighted to invite you to contribute to our new Special Issue, “Organoid-Based Virus Research”. Organoid technology represents a major breakthrough in human biology in the past decade. Organoids are 3D cellular clusters derived from primary tissues or stem cells, consisting of organ-specific cell types and retaining architectural and functional attributes of pertinent tissues and organs. Thus, they are known as mini-organs or organs in a dish. Organoids enable scientists to recapitulate and study in vivo biological processes in culture plates, opening a new arena for biomedical research. Similarly to their vast applications in basic, translational and clinical sciences, organoids have entered the toolbox of virologists and become robust and physiologically relevant tools for studying virus infections and virus–host interaction.

This Special Issue aims to create a forum for scientists to communicate insights and experiences of organoid-based investigations of virus infections and virus–host interaction. We hope that this Special Issue will enable more virologists to understand and realize the great potential of organoids for virus research.

In this Special Issue, original research articles, reviews, and commentaries related to our topics are welcome. Research areas may include (but are not limited to) the following: 1) establishment of organoids for modeling virus infections; 2) organoid-based investigation of viral infections, viral pathogenesis, and virus–host interaction; 3) organoid-based identification of antivirals and/or therapeutic interventions.

We look forward to receiving your contributions.

Dr. Jie Zhou
Dr. Hin Chu
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Viruses is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Published Papers (2 papers)

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Research

20 pages, 12331 KiB  
Article
Non-Polio Enterovirus C Replicate in Both Airway and Intestine Organotypic Cultures
by Giulia Moreni, Hetty van Eijk, Gerrit Koen, Nina Johannesson, Carlemi Calitz, Kimberley Benschop, Jeroen Cremer, Dasja Pajkrt, Adithya Sridhar and Katja Wolthers
Viruses 2023, 15(9), 1823; https://doi.org/10.3390/v15091823 - 27 Aug 2023
Cited by 4 | Viewed by 1664
Abstract
Non-polio enteroviruses (EV) belonging to species C, which are highly prevalent in Africa, mainly among children, are poorly characterized, and their pathogenesis is mostly unknown as they are difficult to culture. In this study, human airway and intestinal organotypic models were used to [...] Read more.
Non-polio enteroviruses (EV) belonging to species C, which are highly prevalent in Africa, mainly among children, are poorly characterized, and their pathogenesis is mostly unknown as they are difficult to culture. In this study, human airway and intestinal organotypic models were used to investigate tissue and cellular tropism of three EV-C genotypes, EV-C99, CVA-13, and CVA-20. Clinical isolates were obtained within the two passages of culture on Caco2 cells, and all three viruses were replicated in both the human airway and intestinal organotypic cultures. We did not observe differences in viral replication between fetal and adult tissue that could potentially explain the preferential infection of infants by EV-C genotypes. Infection of the airway and the intestinal cultures indicates that they both can serve as entry sites for non-polio EV-C. Ciliated airway cells and enterocytes are the target of infection for all three viruses, as well as enteroendocrine cells for EV-C99. Full article
(This article belongs to the Special Issue Organoid-Based Virus Research)
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12 pages, 3439 KiB  
Article
Apical-Out Human Airway Organoids Modeling SARS-CoV-2 Infection
by Man Chun Chiu, Shuxin Zhang, Cun Li, Xiaojuan Liu, Yifei Yu, Jingjing Huang, Zhixin Wan, Xiaoxin Zhu and Jie Zhou
Viruses 2023, 15(5), 1166; https://doi.org/10.3390/v15051166 - 14 May 2023
Cited by 7 | Viewed by 2636
Abstract
The respiratory epithelium, particularly the airway epithelium, is the primary infection site for respiratory pathogens. The apical surface of epithelial cells is constantly exposed to external stimuli including invading pathogens. Efforts have been made to establish organoid cultures to recapitulate the human respiratory [...] Read more.
The respiratory epithelium, particularly the airway epithelium, is the primary infection site for respiratory pathogens. The apical surface of epithelial cells is constantly exposed to external stimuli including invading pathogens. Efforts have been made to establish organoid cultures to recapitulate the human respiratory tract. However, a robust and simple model with an easily accessible apical surface would benefit respiratory research. Here, we report the generation and characterization of apical-out airway organoids from the long-term expandable lung organoids that we previously established. The apical-out airway organoids morphologically and functionally recapitulated the human airway epithelium at a comparable level to the apical-in airway organoids. Moreover, apical-out airway organoids sustained productive and multicycle replication of SARS-CoV-2, and accurately recapitulated the higher infectivity and replicative fitness of the Omicron variants BA.5 and B.1.1.529 and an ancestral virus. In conclusion, we established a physiologically relevant and convenient apical-out airway organoid model for studying respiratory biology and diseases. Full article
(This article belongs to the Special Issue Organoid-Based Virus Research)
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