Radiotherapy Side Effects: Comprehensive Proteomic Study Unraveled Neural Stem Cell Degenerative Differentiation upon Ionizing Radiation
Abstract
:1. Introduction
2. Results
2.1. NSC Proteomic Pattern Profiling Following Different Treatments
2.2. Functional Annotation of Differentially Expressed Proteins
2.3. Irradiation Influenced the Proliferation Capacity, Cell Cycle, and Stemness of NSCs
2.4. Irradiation Impeded NSC Differentiation and Altered Neurogenesis-Associated Protein Expression
2.5. Construction of the Redox-Protein Profile in Irradiated NSCs Via Iodoacetyl-Labelled Mass Spectrometry
3. Discussion
4. Materials and Methods
4.1. Cells and X-ray Irradiation
4.2. qRT-PCR Analysis
4.3. Cell Cycle Analysis
4.4. BrdU Assay
4.5. Immunofluorescence Staining
4.6. Protein and LC MS/MS and TMT Label
4.7. Bioinformatic Analysis
5. Conclusions
6. Patents
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Liang, D.; Ning, M.; Xie, H.; He, X.; Ren, P.; Lei, X.; Zhang, X. Radiotherapy Side Effects: Comprehensive Proteomic Study Unraveled Neural Stem Cell Degenerative Differentiation upon Ionizing Radiation. Biomolecules 2022, 12, 1759. https://doi.org/10.3390/biom12121759
Liang D, Ning M, Xie H, He X, Ren P, Lei X, Zhang X. Radiotherapy Side Effects: Comprehensive Proteomic Study Unraveled Neural Stem Cell Degenerative Differentiation upon Ionizing Radiation. Biomolecules. 2022; 12(12):1759. https://doi.org/10.3390/biom12121759
Chicago/Turabian StyleLiang, Dong, Meng Ning, Hang Xie, Xiaoyan He, Peigen Ren, Xiaohua Lei, and Xuepei Zhang. 2022. "Radiotherapy Side Effects: Comprehensive Proteomic Study Unraveled Neural Stem Cell Degenerative Differentiation upon Ionizing Radiation" Biomolecules 12, no. 12: 1759. https://doi.org/10.3390/biom12121759