Rhabdomyolysis-Induced AKI Was Ameliorated in NLRP3 KO Mice via Alleviation of Mitochondrial Lipid Peroxidation in Renal Tubular Cells
Abstract
:1. Introduction
2. Results
2.1. NLRP3 KO Mice Were Protected from RIAKI
2.2. NLRP3 Depletion in Renal Tubular Cells Mitigated Myoglobin-Induced Ferroptosis and Apoptosis
2.3. NLRP3 Depletion in Renal Tubular Cells Attenuated Myoglobin-Induced Mitochondrial Injury
2.4. NLRP3 Depletion Contributed to Decreasing Myoglobin-Induced ROS Levels and Lipid Peroxidation
3. Discussion
4. Material and Methods
4.1. Animal Models
4.2. Blood Chemistry
4.3. Histopathology
4.4. TdT Mediated dUTP Nick End Labeling (TUNEL) Assay
4.5. Real-Time PCR
4.6. Western Blot
4.7. Cell Culture
4.8. Small Interfering RNA Knockdown Experiments
4.9. MTT Assay
4.10. Flow Cytometric Analysis
4.11. Immunocytochemistry
4.12. Lipid Peroxidation Product Assay
4.13. Statistical Analysis
Author Contributions
Funding
Conflicts of Interest
References
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Song, S.J.; Kim, S.-m.; Lee, S.-h.; Moon, J.-Y.; Hwang, H.S.; Kim, J.S.; Park, S.-H.; Jeong, K.H.; Kim, Y.G. Rhabdomyolysis-Induced AKI Was Ameliorated in NLRP3 KO Mice via Alleviation of Mitochondrial Lipid Peroxidation in Renal Tubular Cells. Int. J. Mol. Sci. 2020, 21, 8564. https://doi.org/10.3390/ijms21228564
Song SJ, Kim S-m, Lee S-h, Moon J-Y, Hwang HS, Kim JS, Park S-H, Jeong KH, Kim YG. Rhabdomyolysis-Induced AKI Was Ameliorated in NLRP3 KO Mice via Alleviation of Mitochondrial Lipid Peroxidation in Renal Tubular Cells. International Journal of Molecular Sciences. 2020; 21(22):8564. https://doi.org/10.3390/ijms21228564
Chicago/Turabian StyleSong, Seok Jong, Su-mi Kim, Sang-ho Lee, Ju-Young Moon, Hyeon Seok Hwang, Jin Sug Kim, Seon-Hwa Park, Kyung Hwan Jeong, and Yang Gyun Kim. 2020. "Rhabdomyolysis-Induced AKI Was Ameliorated in NLRP3 KO Mice via Alleviation of Mitochondrial Lipid Peroxidation in Renal Tubular Cells" International Journal of Molecular Sciences 21, no. 22: 8564. https://doi.org/10.3390/ijms21228564