Antibacterial Peptide NP-6 Affects Staphylococcus aureus by Multiple Modes of Action
Abstract
:1. Introduction
2. Results and Discussion
2.1. Physicochemical Properties of NP-6
2.1.1. Hemolysis Activity of NP-6
2.1.2. Cytotoxicity of NP-6
2.1.3. Serum Stability of NP-6
2.2. TEM Observations
2.3. PI Uptake Analysis
2.4. Binding Ability of NP-6 with Bacterial DNA and RNA
2.5. Effect of NP-6 on the Activities of Intracellular T-ATPase and β-Galactosidase
3. Material and Methods
3.1. Materials
3.2. Physicochemical Properties of NP-6
3.2.1. Hemolysis Assay
3.2.2. Cytotoxicity Assay
3.2.3. Serum Stability
3.3. Observation of Transmission Electron Microscopy (TEM)
3.4. Confocal Laser Scanning Microscopy (CLSM) Test
3.5. Flow Cytometric Analysis
3.6. Competitive Binding of NP-6 and EB with Bacterial DNA
3.7. DNA Gel Retardation
3.8. RNA Gel Retardation
3.9. Effect of NP-6 on the Activity of T-ATPase
3.10. Effect of NP-6 on the Activity of β-Galactosidase
3.11. Statistical Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Hou, X.; Li, J.; Tang, H.; Li, Q.; Shen, G.; Li, S.; Chen, A.; Peng, Z.; Zhang, Y.; Li, C.; et al. Antibacterial Peptide NP-6 Affects Staphylococcus aureus by Multiple Modes of Action. Int. J. Mol. Sci. 2022, 23, 7812. https://doi.org/10.3390/ijms23147812
Hou X, Li J, Tang H, Li Q, Shen G, Li S, Chen A, Peng Z, Zhang Y, Li C, et al. Antibacterial Peptide NP-6 Affects Staphylococcus aureus by Multiple Modes of Action. International Journal of Molecular Sciences. 2022; 23(14):7812. https://doi.org/10.3390/ijms23147812
Chicago/Turabian StyleHou, Xiaoyan, Jianlong Li, Huaqiao Tang, Qingye Li, Guanghui Shen, Shanshan Li, Anjun Chen, Zixin Peng, Yu Zhang, Chaowei Li, and et al. 2022. "Antibacterial Peptide NP-6 Affects Staphylococcus aureus by Multiple Modes of Action" International Journal of Molecular Sciences 23, no. 14: 7812. https://doi.org/10.3390/ijms23147812