Phosphoproteome Reveals Extracellular Regulated Protein Kinase Phosphorylation Mediated by Mitogen-Activated Protein Kinase Kinase-Regulating Granulosa Cell Apoptosis in Broody Geese
Abstract
:1. Introduction
2. Results
2.1. Morphological Detection and Antioxidant Activity of Ovaries in Broody Geese
2.2. MS-Based Quantitative Proteomic and Phosphoproteomic Characterizations of the Ovaries of Laying and Broody Geese
2.3. Identification of Signaling Pathways and Key Substrate of Differential Abundance Phosphoproteins between Laying and Broody Geese
2.4. Functional Validation of Specific Kinase (MEK)
2.5. Regulation of Granulosa Cell Apoptosis by MEK-Mediated Phosphorylation of ERK
3. Discussion
4. Materials and Methods
4.1. Animal and Sample Collection
4.2. Morphological Detection and Antioxidant Activity of Ovaries
4.3. Proteome and Phosphoproteome Analyses
4.3.1. Protein Extraction
4.3.2. Protein Digestion and TMT Labeling
4.3.3. LC-MS/MS Analysis and Database Search
4.3.4. Bioinformatics Analysis
4.4. Isolation and Identification of Goose Ovarian Granulosa Cells
4.5. Obtaining Overexpression Vectors and MEK siRNA, and Transfecting Them
4.6. RNA Extraction, cDNA Synthesis, and Quantitative Real-Time PCR
4.7. Capillary Western Blotting
4.8. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Zhao, S.; Gu, T.; Weng, K.; Zhang, Y.; Cao, Z.; Zhang, Y.; Zhao, W.; Chen, G.; Xu, Q. Phosphoproteome Reveals Extracellular Regulated Protein Kinase Phosphorylation Mediated by Mitogen-Activated Protein Kinase Kinase-Regulating Granulosa Cell Apoptosis in Broody Geese. Int. J. Mol. Sci. 2023, 24, 12278. https://doi.org/10.3390/ijms241512278
Zhao S, Gu T, Weng K, Zhang Y, Cao Z, Zhang Y, Zhao W, Chen G, Xu Q. Phosphoproteome Reveals Extracellular Regulated Protein Kinase Phosphorylation Mediated by Mitogen-Activated Protein Kinase Kinase-Regulating Granulosa Cell Apoptosis in Broody Geese. International Journal of Molecular Sciences. 2023; 24(15):12278. https://doi.org/10.3390/ijms241512278
Chicago/Turabian StyleZhao, Shuai, Tiantian Gu, Kaiqi Weng, Yu Zhang, Zhengfeng Cao, Yang Zhang, Wenming Zhao, Guohong Chen, and Qi Xu. 2023. "Phosphoproteome Reveals Extracellular Regulated Protein Kinase Phosphorylation Mediated by Mitogen-Activated Protein Kinase Kinase-Regulating Granulosa Cell Apoptosis in Broody Geese" International Journal of Molecular Sciences 24, no. 15: 12278. https://doi.org/10.3390/ijms241512278